Search Results - "LeMaster, D M"

Reduced Temperature Dependence of Collective Conformational Opening in a Hyperthermophile Rubredoxin by Hernández, Griselda, LeMaster, David M

“…Spatially localized differences in the conformational dynamics of the rubredoxins from the hyperthermophile Pyrococcus furiosus (Pf) and the mesophile…”
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Unusual NMR Multiplet Structures of Spin-1/2 Nuclei Coupled to Spin-1 Nuclei by London, R. E, LeMaster, D. M, Werbelow, L. G

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Deuteration in protein proton magnetic resonance by LeMaster, D M

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Millisecond Time Scale Conformational Flexibility in a Hyperthermophile Protein at Ambient Temperature by Hernandez, Griselda, Jenney, Francis E., Michael W. W. Adams, LeMaster, David M.

“…Rubredoxin from the hyperthermophile Pyrococcus furiosus is the most thermostable protein characterized to date with an estimated global unfolding rate of…”
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Nucleotide sequence and protein overproduction of bacteriophage T4 thioredoxin by LEMASTER, D. M

“…Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Protein dynamics and distance determination by NOE measurements by LeMaster, David M., Kay, Lewis E., Brünger, Axel T., Prestegard, J.H.

“…Present analysis procedures for NMR structure determination of macromolecules presuppose fixed internuclear distances. Improvement of the precision of the…”
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The Use of Multispectral Imaging in DIEP Free Flap Perforator Selection: A Case Study by Rammos, Charalambos K., Jones, Glyn E., Taege, Sara M., Lemaster, Chelsey M.

“…SUMMARYPerforator selection is of paramount importance when performing a Deep Inferior Epigastric Perforator flap. Technological advancements within imaging…”
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Protein NMR resonance assignment by isotropic mixing experiments on random fractionally deuterated samples by LeMaster, David M.

“…The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e…”
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Biosynthetic production of 13C-labeled amino acids with site-specific enrichment by D M LeMaster, J E Cronan, Jr

“…We have developed two Escherichia coli strains for the production of specifically labeled amino acids suitable for high resolution nuclear magnetic resonance…”
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Uniform and selective deuteration in two-dimensional NMR of proteins by LEMASTER, D. M

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Structural Determinants of the Catalytic Reactivity of the Buried Cysteine of Escherichia coli Thioredoxin by LeMaster, David M

“…The structurally homologous thioredoxins and thioltransferases/glutaredoxins possess a solvent-exposed cysteine sulfur which carries out a nucleophilic attack…”
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The role of the buried aspartate of Escherichia coli thioredoxin in the activation of the mixed disulfide intermediate by LeMaster, D M, Springer, P A, Unkefer, C J

“…The structurally homologous protein disulfide isomerases and thioredoxins exhibit a 10(5) variation of redox equilibria. It is demonstrated that the kinetic…”
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NMR sequential assignment of Escherichia coli thioredoxin utilizing random fractional deuteriation by LeMaster, David M, Richards, Frederic M

“…All non-proline residues except for the N-terminal dipeptide have been assigned in the 108-residue protein Escherichia coli thioredoxin. Central to these…”
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Deuterium labelling in NMR structural analysis of larger proteins by LeMaster, D M

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Chiral β and random fractional deuteration for the determination of protein sidechain conformation by NMR by LeMaster, David M.

“…Stereospecific assignments of the aspartic acid and asparagine, β-protons of the 108 residue protein E. coli thioredoxin have been obtained by the use of…”
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The 3$^{\prime}$ Splice Site of Pre-Messenger RNA is Recognized by a Small Nuclear Ribonucleoprotein by Chabot, Benoit, Black, Douglas L., LeMaster, David M., Steitz, Joan A.

“…A component present in splicing extracts selectively binds the 3′ splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene…”
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Larmor frequency selective model free analysis of protein NMR relaxation by Lemaster, D M

“…The Lipari-Szabo dynamical formalism is extended by setting the time constants of the Lorentzian terms to [Formula: see text] and [Formula: see text]. This…”
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The use of cystathionine gamma-synthase in the production of alpha and chiral beta deuterated amino acids by Homer, R J, Kim, M S, LeMaster, D M

“…Chiral exchange at the alpha hydrogen position can be carried out on gram scale in 2H2O for all of the standard amino acids utilizing Escherichia coli…”
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Assessment of protein solution versus crystal structure determination using spin-diffusion-suppressed NOE and heteronuclear relaxation data by LeMaster, D M

“…A spin-diffusion-suppressed NOE buildup series has been measured for E. coli thioredoxin. The extensive 13C and 15N relaxation data previously reported for…”
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Synthesis of alpha, beta-deuterated 15N amino acids using a coupled glutamate dehydrogenase-branched-chain amino acid aminotransferase system by Chanatry, J A, Schafer, P H, Kim, M S, LeMaster, D M

“…Gram amounts of various 15N-enriched L-amino acids have been synthesized using a coupled enzymatic system. Catalytic amounts of 15N-labeled L-glutamate are…”
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