Raman cell imaging with boron cluster molecules conjugated with biomolecules

Raman cell imaging with boron cluster molecules conjugated with biomolecules

Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B-H stretching vibrations of two types of simple icosahedral boron clusters, ortho -carborane 3 and closo -dodecaborate 4 appeared at approximately 2450-2700 cm −1 , and did not overlap wi...

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Bibliographic Details
Published in:RSC advances Vol. 9; no. 41; pp. 23973 - 23978
Main Authors: Mochizuki, Masahito, Sato, Shinichi, Asatyas, Syifa, Le nikowski, Zbigniew J, Hayashi, Tomohiro, Nakamura, Hiroyuki
Format: Journal Article
Language:English
Published: England Royal Society of Chemistry 02-08-2019
The Royal Society of Chemistry
Subjects:
Alkynes
Band theory
Biomolecules
Boron
Carborane
Chemistry
Cholesterol
Clusters
Icosahedral phase
Imaging
Spectrum analysis
Stretching
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Summary:Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B-H stretching vibrations of two types of simple icosahedral boron clusters, ortho -carborane 3 and closo -dodecaborate 4 appeared at approximately 2450-2700 cm −1 , and did not overlap with those of cellular components. Although ortho -carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho -carborane derivative 6 , which internally has an alkyne moiety, exhibited very weak Raman signals of the C&z.tbd;C stretching and the B-H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In contrast, closo -dodecaborate derivatives such as BSH ( 5 ) were found to be a potential Raman imaging probe cluster for target molecules in the cell. BSH-conjugated cholesterol 7 (BSH-Chol) was synthesized and used in Raman imaging in cells. Raman imaging and spectral analysis revealed that BSH-based Raman tags provide a versatile platform for quantitative Raman imaging. We performed Raman cell imaging using boron clusters.
Bibliography:10.1039/c9ra04228h
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:2046-2069
2046-2069
DOI:10.1039/c9ra04228h