Search Results - "Le Roy, Katrien"
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X‐ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1‐exohydrolase IIa of Cichorium intybus
Published in The Plant journal : for cell and molecular biology (01-02-2005)“…Summary Fructan 1‐exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1‐FEH IIa…”
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Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase
Published in The New phytologist (01-05-2008)“…The hydrolytic plant enzymes of family 32 of glycoside hydrolases (GH32), including acid cell wall type invertases (EC 3.2.1.26), fructan 1-exohydrolases…”
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Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications
Published in Journal of experimental botany (01-03-2009)“…Glycoside hydrolases (GH) have been shown to play unique roles in various biological processes like the biosynthesis of glycans, cell wall metabolism, plant…”
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A Single Active Trehalose-6-P Synthase (TPS) and a Family of Putative Regulatory TPS-Like Proteins in Arabidopsis
Published in Molecular plant (01-03-2010)“…Higher plants typically do not produce trehalose in large amounts, but their genome sequences reveal large families of putative trehalose metabolism enzymes…”
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Fructan metabolism in developing wheat (Triticum aestivum L.) kernels
Published in Plant and cell physiology (01-12-2013)“…Although fructans play a crucial role in wheat kernel development, their metabolism during kernel maturation is far from being understood. In this study, all…”
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Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes
Published in The FEBS journal (01-10-2009)“…Plant family 32 glycoside hydrolase enzymes include hydrolases (cell wall invertases, fructan exohydrolases, vacuolar invertases) and fructosyltransferases…”
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pKa modulation of the acid/base catalyst within GH32 and GH68: a role in substrate/inhibitor specificity?
Published in PloS one (25-05-2012)“…Glycoside hydrolases of families 32 (GH32) and 68 (GH68) belong to clan GH-J, containing hydrolytic enzymes (sucrose/fructans as donor substrates) and…”
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1-FFT amino acids involved in high DP inulin accumulation in Viguiera discolor
Published in Frontiers in plant science (11-08-2015)“…Fructans are important vacuolar reserve carbohydrates with drought, cold, ROS and general abiotic stress mediating properties. They occur in 15% of all…”
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Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor
Published in The New phytologist (01-01-2007)“…Invertases and fructan exohydrolases (FEHs) fulfil important physiological functions in plants. Sucrose is the typical substrate for invertases and bacterial…”
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Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
Published in Journal of experimental botany (01-01-2007)“…Fructans, which are β-(2,1) and/or β-(2,6) linked polymers of fructose, are important storage carbohydrates in many plants. They are mobilized via fructan…”
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N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleft
Published in The New phytologist (01-01-2007)“…Recently, the three-dimensional structure of chicory (Cichorium intybus) fructan 1-exohydrolase (1-FEH IIa) in complex with its preferential substrate,…”
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Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes
Published in Journal of experimental botany (01-03-2006)“…Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural…”
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Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities
Published in Plant, cell and environment (01-04-2005)“…ABSTRACT The genome of Arabidopsis thaliana contains six putative cell‐wall type invertase genes (AtcwINV1‐6). Heterologous expression of AtcwINV1, 3 and 6…”
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Unraveling the Difference between Invertases and Fructan Exohydrolases: A Single Amino Acid (Asp-239) Substitution Transforms Arabidopsis Cell Wall Invertase1 into a Fructan 1-Exohydrolase1[C]
Published in Plant physiology (Bethesda) (01-11-2007)“…Plant cell wall invertases and fructan exohydrolases (FEHs) are very closely related enzymes at the molecular and structural level (family 32 of glycoside…”
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Sugar signalling and antioxidant network connections in plant cells
Published in The FEBS journal (01-05-2010)“…Sugars play important roles as both nutrients and regulatory molecules throughout plant life. Sugar metabolism and signalling function in an intricate network…”
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Exploring the neutral invertase–oxidative stress defence connection in Arabidopsis thaliana
Published in Journal of experimental botany (01-07-2011)“…Over the past decades, considerable advances have been made in understanding the crucial role and the regulation of sucrose metabolism in plants. Among the…”
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Understanding the Role of Defective Invertases in Plants: Tobacco Nin88 Fails to Degrade Sucrose
Published in Plant physiology (Bethesda) (01-04-2013)“…Cell wall invertases (cwINVs), with a high affinity for the cell wall, are fundamental enzymes in the control of plant growth, development, and carbon…”
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Unexpected Presence of Graminan- and Levan-Type Fructans in the Evergreen Frost-Hardy Eudicot Pachysandra terminalis (Buxaceae): Purification, Cloning, and Functional Analysis of a 6-SST/6-SFT Enzyme
Published in Plant physiology (Bethesda) (01-01-2011)“…About 15% of flowering plants accumulate fructans. Inulin-type fructans with β(2,1) fructosyl linkages typically accumulate in the core eudicot families (e.g…”
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Unraveling the Difference between Invertases and Fructan Exohydrolases: A Single Amino Acid (Asp-239) Substitution Transforms Arabidopsis Cell Wall Invertase1 into a Fructan 1-Exohydrolase
Published in Plant physiology (Bethesda) (01-11-2007)“…Plant cell wall invertases and fructan exohydrolases (FEHs) are very closely related enzymes at the molecular and structural level (family 32 of glycoside…”
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Crystal Structures of Arabidopsis thaliana Cell-Wall Invertase Mutants in Complex with Sucrose
Published in Journal of molecular biology (21-03-2008)“…In plants, cell-wall invertases fulfil important roles in carbohydrate partitioning, growth, development and crop yield. In this study, we report on different…”
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