Development of a highly sensitive magneto-enzyme lateral flow immunoassay for dengue NS1 detection

Development of a highly sensitive magneto-enzyme lateral flow immunoassay for dengue NS1 detection

Dengue infection represents a global health issue of growing importance. Dengue non-structural protein 1 (NS1) plays a central role in the early detection of the disease. The most common method for NS1 detection is testing by lateral flow immunoassays (LFIAs) with varying sensitivity. In this study,...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) Vol. 7; p. e7779
Main Authors: Tran, Tien V, Nguyen, Ba V, Nguyen, Thao T P, Tran, Tung T, Pham, Khanh G, Le, Quang B, Do, Binh N, Pham, Hung N, Nguyen, Chuyen V, Dinh, Duong P H, Ha, Van T, Doan, Trang H T, Le, Hoa Q
Format: Journal Article
Language:English
Published: United States PeerJ. Ltd 25-09-2019
PeerJ, Inc
PeerJ Inc
Subjects:
Antibodies
Antigens
Biotechnology
Biotin
Cellulose esters
Cross-reactivity
Dengue fever
Dengue virus
Diseases
Encephalitis
Enzymes
Explosives
Health
Health aspects
Hepatitis
Hepatitis B
Hepatitis B virus
Hepatitis C
Hepatitis C virus
Immunoassay
Immunoglobulins
Infection
Infectious Diseases
Japanese encephalitis
Magnetic lateral flow immunoassay
Medical research
Nanoparticles
Novels
NS1 protein
Rapid method
Serotypes
Signal amplification
Streptavidin
World health
Israel
California
France
Japan
Vietnam
St Louis Missouri
United States > US
Rapid method
Magnetic lateral flow immunoassay
Dengue virus
NS1 protein
Signal amplification
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Summary:Dengue infection represents a global health issue of growing importance. Dengue non-structural protein 1 (NS1) plays a central role in the early detection of the disease. The most common method for NS1 detection is testing by lateral flow immunoassays (LFIAs) with varying sensitivity. In this study, we present a highly sensitive magneto-enzyme LFIA for prompt diagnosis of dengue. We have demonstrated the development of a magneto-enzyme LFIA combining super-paramagnetic nanoparticles as labels and Biotin-Streptavidin signal amplification strategy to detect dengue NS1. Factors affecting the test performance including antibody pair, super-paramagnetic nanoparticle size, nitrocellulose membrane type, amounts of detection and capture antibodies, and amounts of Streptavidin-polyHRP were optimized. Analytical sensitivity and cross-reactivity were determined. Clinical performance of the novel assay was evaluated using a panel of 120 clinical sera. This newly developed assay could detect NS1 of all four serotypes of dengue virus (DENV). The limit of detection (LOD) was found to be as low as 0.25 ng ml for DENV-1 and DENV-3, 0.1 ng ml for DENV-2, and 1.0 ng ml for DENV-4. The LOD for DENV-2 was a 50-fold improvement over the best values previously reported. There was an absence of cross-reactivity with Zika NS1, Hepatitis B virus, Hepatitis C virus, and Japanese encephalitis virus. The sensitivity and specificity of the novel assay were 100% when tested on clinical samples. We have successfully developed a magneto-enzyme LFIA, allowing rapid and highly sensitive detection of dengue NS1, which is essential for proper management of patients infected with DENV.
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ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.7779