Search Results - "Lazar, Dan F"

A live-cell assay for the real-time assessment of extracellular ATP levels by Niles, Andrew L., Cali, James J., Lazar, Dan F.

“…Extracellular ATP (eATP) is a potent damage associated molecular pattern (DAMP) molecule known to exert profound effects on the innate and adaptive immune…”
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Lipid phosphatases as drug discovery targets for type 2 diabetes by Lazar, Dan F, Saltiel, Alan R

“…The soaring incidence of type 2 diabetes has created pressure for new pharmaceutical strategies to treat this devastating disease. With much of the focus on…”
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Multimodal assessment of autophagy in mammalian cells with a novel, LC3-based tandem reporter by Lazar, Dan F, Gillette, Amani A, Lewis, Samantha R, Butler, Braeden L

“…Autophagy is an important intracellular pathway for the degradation of superfluous or harmful subcellular materials, thereby playing a critical role in the…”
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Mitogen-activated Protein Kinase Kinase Inhibition Does Not Block the Stimulation of Glucose Utilization by Insulin (∗) by Lazar, Dan F., Wiese, Russell J., Brady, Matthew J., Mastick, Cynthia Corley, Waters, Steven B., Yamauchi, Keishi, Pessin, Jeffrey E., Cuatrecasas, Pedro, Saltiel, Alan R.

“…Insulin stimulates the activity of mitogen-activated protein kinase (MAPK) via its upstream activator, MAPK kinase (MEK), a dual specificity kinase that…”
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Differential T Cell Responsiveness to Proliferative Stimuli Assessed with a Novel, Homogeneous Ki-67 Immunoassay by Lazar, Dan F, Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…Abstract Proliferative capacity is an important component of T cell fitness and a relevant parameter for characterization of CAR T cells and T cell activating…”
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Abstract 1933: Improved DAMP assays for the in vitro assessment of immunogenic cell death by Niles, Andrew L., Kupcho, Kevin R., Lazar, Dan F., Cali, James J.

“…Abstract The extracellular levels of damage-associated molecular patterns (DAMPs) released during immunogenic cell death (ICD) are positively correlated with…”
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Abstract 1832: A real-time extracellular ATP assay to identify modulators of the innate immune response by Niles, Andrew L., Kupcho, Kevin R., Lazar, Dan F., Cali, James J.

“…Abstract Damage-associated molecular patterns (DAMPs) liberated or exposed by dying cells after chemotherapeutic treatment augment innate immune responses and…”
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Lumit&[trade] Immunoassays for Simple and Rapid Determination of Cytokine Release from Cell Culture Models by Lazar, Dan F, Hsiao, Kevin, Sondgeroth, Casey, Gilden, Julia, Kupcho, Kevin, Thompson, David, O’Brien, Martha, Cali, James

“…Abstract Rapid (< 70 min), add-and-read assays for cytokine determination were developed with Lumit™ immunoassay technology. Separate antibodies labeled with…”
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Abstract 710: Real-time apoptosis and necrosis detection in 3D spheroid cellmodels by Niles, Andrew L., Kupcho, Kevin R., Riss, Terry L., Lazar, Dan F., Cali, James J.

“…Abstract The structural and biological complexity of cancer spheroids make them uniquely suited to address or approximate the pharmacodynamics of new chemical…”
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Abstract 1631: A homogeneous assay system for discernment of proliferative, antiproliferative, and cytotoxic effects in culture by Lazar, Dan F., Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…Abstract We describe a novel homogeneous cell-based assay developed for the quantitative detection of Ki-67 protein, a recognized marker of cell proliferation,…”
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Abstract 5595: Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F., Kupcho, Kevin R., Sondgeroth, Casey A., Thompson, David V., O'Brien, Martha A., Gilden, Julia K., Hsiao, Kevin, Cali, James J.

“…Abstract Cytokines play a major role in cancer biology as inflammatory and immunomodulatory agents and are frequently measured in cell culture models and other…”
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Rapid and sensitive determination of cytokine release from cells without the need for sample transfer by Lazar, Dan F, Kupcho, Kevin R, Sondgeroth, Casey A, Hsiao, Kevin, Thompson, David V, O’Brien, Martha A, Gilden, Julia K, Cali, James J

“…Abstract We have utilized NanoLuc® Binary Technology (NanoBiT®) to develop a homogeneous and rapid assay method (≤ 70 min completion time) to measure cytokines…”
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Abstract 1428: Luminescent immunoassays for homogeneous determination of cell proliferation and senescence by Lazar, Dan F., Kupcho, Kevin R., Niles, Andrew L., Cali, James J.

“…Abstract We have developed homogeneous, luminescent immunoassays for simple and rapid determination of Ki-67 and uPAR levels in 96-well format, thereby…”
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Abstract 1340: Assessing autophagic flux in cell culture models with a novel plate-based assay by Lazar, Dan F., Kessens, Ryan W., Gillette, Amani A., Butler, Braeden L., Eggers, Christopher T., Binkowski, Brock F., Vidugiris, Gediminas, Slater, Michael R., Ma, Dongping, Cali, James J.

“…Abstract The importance of autophagy in normal cell health and diseased states, including cancer, immunology and inflammation, has become quite clear. Given…”
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Stimulation of Glycogen Synthesis by Insulin in Human Erythroleukemia Cells Requires the Synthesis of Glycosyl-Phosphatidylinositol by Lazar, Dan F., Knez, Jansen J., Medof, M. Edward, Cuatrecasas, Pedro, Saltiel, Alan R.

“…Although the insulin-dependent hydrolysis of glycosyl-phosphatidylinositol (GPI) may play an important role in insulin action, an absolute requirement for this…”
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Abstract 3312: A novel plate-based assay for screening autophagic activity in 2D and 3D cell culture models by Lazar, Dan F., Gillette, Amani A., Butler, Braeden L., Eggers, Christopher T., Binkowski, Brock F., Vidugiris, Gediminas, Slater, Michael R., Ma, Dongping, Cali, James J.

“…Abstract The critical importance of autophagy in cell health and its proposed role in disease-relevant biology, including cancer, inflammation, and immunology,…”
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Abstract 4784: Selective bioluminogenic HDAC activity assays for profiling HDAC inhibitors by Kupcho, Kevin R., Evans, Nathan J., Niles, Andrew L., Kirkland, Thomas A., Lazar, Dan F.

“…Abstract Histone deacetylases (HDACs) play critical roles in the regulation of gene transcription and cell signaling events by deacetylating histones and other…”
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Lipid Bilayer in Genetic Hypertension by Dominiczak, Anna F, Lazar, Dan F, Das, Arun K, Bohr, David F

“…Membrane microviscosity, phospholipid composition, and turnover were measured in cultured vascular smooth muscle cells isolated from mesenteric arteries of…”
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Abstract 4237: Isoenzyme-selective HDAC activity assays by Kupcho, Kevin R., Evans, Nathan J., Niles, Andrew L., Lazar, Dan F., Kirkland, Thomas A.

“…Abstract Histone deacetylases (HDACs) are the family of enzymes which catalyze removal of acetyl groups from lysine residues on both histone and non-histone…”
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Abstract 5531: A bioluminescent cell viability assay optimized for 3D microtissues by Valley, Michael P., Zimprich, Chad, Cali, James J., Lazar, Dan F.

“…Abstract It has been well demonstrated that the morphology, gene expression, and overall biological response of cells assessed in 3-dimensional (3D) culture…”
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