Recombinant immunosuppressive protein from Pimpla hypochondrica venom (rVPr1) increases the susceptibility of Mamestra brassicae larvae to the fungal biological control agent, Beauveria bassiana

Although fungi are used to control a variety of insect pests, it is accepted that their usage could be increased if their efficacy was greater. The outcome of the interaction of a fungus and a pest insect may be influenced by a number of criteria, including the ability of the insect to mount effecti...

Full description

Saved in:

Bibliographic Details
Published in:	Archives of insect biochemistry and physiology Vol. 78; no. 3; pp. 119 - 131
Main Authors:	Richards, Elaine H, Bradish, Hannah, Dani, M. Paulina, Pietravalle, Stéphane, Lawson, Ailsa
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 01-11-2011
Subjects:	Animals Beauveria - physiology Beauveria bassiana bioassays Biological control biological control agents Cell Aggregation - drug effects Conidia Fungi Hemocytes - drug effects Hymenoptera Immune response Immunosuppressive Agents - pharmacology immunosuppressive protein insect pests Insect Proteins - pharmacology integrated pest management Larva - drug effects Larva - microbiology Larvae Lepidoptera Mamestra brassicae Moths - drug effects Moths - microbiology Pathogens Pest Control Pests Pimpla Pimpla hypochondriaca Recombinant Proteins - pharmacology rVPr1 Survival Topical application Venom venoms Wasp Venoms - pharmacology
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Although fungi are used to control a variety of insect pests, it is accepted that their usage could be increased if their efficacy was greater. The outcome of the interaction of a fungus and a pest insect may be influenced by a number of criteria, including the ability of the insect to mount effective immune responses against the pathogen. In view of this, we aimed to determine if a recombinant immunosuppressive wasp venom protein (rVPr1) can increase the susceptibility of larvae of the lepidopteran pest, Mamestra brassicae, to the fungal biological control agent, Beauveria bassiana. Bioassays indicated that when larvae were injected with 3.5 µl of rVPr1 and 100 B. bassiana conidia (combined injection assays), a significant reduction in survival of larvae occurred compared with each treatment on its own (P = 0.006). Similar results were obtained when larvae were dipped in a solution containing 3 × 106 B. bassiana conidia per ml and then injected with 3.5 µl of rVPr1 2 days later (topical application assays), (P<0.001). These results indicate that rVPr1 can increase the efficacy of B. bassiana toward a lepidopteran pest, and are discussed within the context of insect immune responses and integrated pest management. © 2011 Wiley Periodicals, Inc.
Bibliography:	http://dx.doi.org/10.1002/arch.20447 ark:/67375/WNG-S4PT8TLB-7 istex:87FA568126DEEF7351A8C6827DF40015405CD9F5 ArticleID:ARCH20447 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0739-4462 1520-6327 1520-6327
DOI:	10.1002/arch.20447