Polyhydroxybutyrate and its Copolymer with Polyhydroxyvalerate as Biomaterials: Influence on Progression of Stem Cell Cycle

Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are biopolyesters reported to provide favorable microenvironments for cell culture and possess potential for tissue engineering applications. Both biopolymers have been investigated for applications in a variety of...

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Published in:	Biomacromolecules Vol. 11; no. 10; pp. 2707 - 2715
Main Authors:	Ahmed, Tania, Marçal, Helder, Lawless, Melissa, Wanandy, Nico S, Chiu, Alex, Foster, L. John R
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 11-10-2010
Subjects:	Animals Apoptosis - drug effects Applied sciences Biocompatible Materials - chemical synthesis Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biological and medical sciences Biological properties Cell Adhesion - drug effects Cell Cycle - drug effects Cell Lineage - drug effects Cells, Cultured DNA - metabolism Exact sciences and technology Hydroxybutyrates - chemistry Mechanical Phenomena Medical sciences Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Microscopy, Confocal Microscopy, Electron, Scanning Neural Stem Cells - cytology Neural Stem Cells - metabolism Olfactory Mucosa - cytology Olfactory Mucosa - metabolism Organic polymers Physicochemistry of polymers Polyesters - chemistry Properties and characterization Surface Properties Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Tissue Engineering - methods Biological properties Cell proliferation Valerate(hydroxy) copolymer Film Stem cell Mechanical properties Butyrate(hydroxy) copolymer Butyrate(hydroxy)polymer Mesenchymal cell Experimental study Morphology Cell adhesion Cell cycle Biomaterial Comparative study Apoptosis
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Summary:	Poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) are biopolyesters reported to provide favorable microenvironments for cell culture and possess potential for tissue engineering applications. Both biopolymers have been investigated for applications in a variety of medical scenarios, including nerve and bone repair. This study investigated the influence these biomaterials exerted on cell cycle progression of olfactory ensheathing cells (OECs) and mesenchymal stem cells (MSCs) commonly used in the engineering of nerve and bone tissues. Cell cycle regulation is important for cell survival; analysis revealed that the biomaterials induced significant cell cycle progression in both MSCs and OECs. Significantly higher percentages of cells were cycled at synthesis (S) phase of the cycle on PHBV films compared to PHB, with MSCs more susceptible than OECs. Furthermore, detection of early stages of apoptotic activation showed significant differences in the two cell populations exhibiting necrosis and apoptosis when cultivated on the biomaterials. OECs compromised on PHB (5.6%) and PHBV (2.5%) compared to MSCs with 12.6% on PHB and 17% on PHBV. Significant differences in crystallinity and surface rugosity were determined between films of the two biomaterials, 88% and 1.12 μm for PHB and 76% and 0.72 μm for PHBV. While changes in surface properties may have influenced cell adhesion, the work presented here suggests that application of these biomaterials in tissue engineering are specific to cell type and requires a detailed investigation at the cell−material interface.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1525-7797 1526-4602
DOI:	10.1021/bm1007579