Conditioned Aversion Study of Sucrose and SC45647 Taste in TRPM5 Knockout Mice

Previously, published studies have reported mixed results regarding the role of the TRPM5 cation channel in signaling sweet taste by taste sensory cells. Some studies have reported a complete loss of sweet taste preference in TRPM5 knockout (KO) mice, whereas others have reported only a partial loss...

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Bibliographic Details
Published in:	Chemical senses Vol. 37; no. 5; pp. 391 - 401
Main Authors:	Eddy, Meghan C, Eschle, Benjamin K, Peterson, Darlene, Lauras, Nathan, Margolskee, Robert F, Delay, Eugene R
Format:	Journal Article
Language:	English
Published:	Oxford Oxford University Press 01-06-2012
Subjects:	acetates Acetic acid Animals Aversion learning Biological and medical sciences Bitter taste cation channels Chemical stimuli Choice Behavior - drug effects Choice Behavior - physiology Conditioning (Psychology) - drug effects Dysgeusia - genetics Dysgeusia - physiopathology Flavor food choices Fundamental and applied biological sciences. Psychology Guanidines - administration & dosage learning Lithium Chloride - administration & dosage Mice Mice, Knockout Odor Odorants odors Olfaction. Taste Original Pentanols - administration & dosage Perception Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology Sensory evaluation Signal transduction Signal Transduction - drug effects Signal Transduction - physiology Smell - drug effects Smell - physiology Sucrose Sucrose - administration & dosage Sweet taste Sweetening Agents - administration & dosage taste Taste - drug effects Taste - physiology Taste aversion Taste Perception - drug effects Taste Perception - physiology transient receptor potential proteins TRPM Cation Channels - deficiency TRPM Cation Channels - genetics Sucrose Rodentia conditioned taste aversion Gustative system Psychophysics Flavor conditioned flavor aversion animal psychophysics Vertebrata Mammalia Acquisition process Mouse Gustation Animal Olfaction Perception Taste Mutation Conditioned aversion
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Summary:	Previously, published studies have reported mixed results regarding the role of the TRPM5 cation channel in signaling sweet taste by taste sensory cells. Some studies have reported a complete loss of sweet taste preference in TRPM5 knockout (KO) mice, whereas others have reported only a partial loss of sweet taste preference. This study reports the results of conditioned aversion studies designed to motivate wild-type (WT) and KO mice to respond to sweet substances. In conditioned taste aversion experiments, WT mice showed nearly complete LiCl-induced response suppression to sucrose and SC45647. In contrast, TRPM5 KO mice showed a much smaller conditioned aversion to either sweet substance, suggesting a compromised, but not absent, ability to detect sweet taste. A subsequent conditioned flavor aversion experiment was conducted to determine if TRPM5 KO mice were impaired in their ability to learn a conditioned aversion. In this experiment, KO and WT mice were conditioned to a mixture of SC45647 and amyl acetate (an odor cue). Although WT mice avoided both components of the stimulus mixture, they avoided SC45647 more than the odor cue. The KO mice also avoided both stimuli, but they avoided the odor component more than SC45647, suggesting that while the KO mice are capable of learning an aversion, to them the odor cue was more salient than the taste cue. Collectively, these findings suggest the TRPM5 KO mice have some residual ability to detect SC45647 and sucrose, and, like bitter, there may be a TRPM5-independent transduction pathway for detecting these substances.
Bibliography:	http://dx.doi.org/10.1093/chemse/bjr093 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1
ISSN:	0379-864X 1464-3553
DOI:	10.1093/chemse/bjr093