A large colonial choanoflagellate from Mono Lake harbors live bacteria

As the closest living relatives of animals, choanoflagellates offer insights into the ancestry of animal cell physiology. Here, we report the isolation and characterization of a colonial choanoflagellate from Mono Lake, California. The choanoflagellate forms large spherical colonies that are an orde...

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Published in:	mBio Vol. 15; no. 9; p. e0162324
Main Authors:	Hake, K H, West, P T, McDonald, K, Laundon, D, Reyes-Rivera, J, Garcia De Las Bayonas, A, Feng, C, Burkhardt, P, Richter, D J, Banfield, J F, King, N
Format:	Journal Article
Language:	English
Published:	United States American Society for Microbiology 11-09-2024
Subjects:	Alphaproteobacteria - classification Alphaproteobacteria - genetics Alphaproteobacteria - isolation & purification bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification California Choanoflagellata - classification Choanoflagellata - physiology choanoflagellates Evolution fluorescence in situ hybridization Gammaproteobacteria - classification Gammaproteobacteria - genetics Gammaproteobacteria - isolation & purification Gammaproteobacteria - physiology Lakes - microbiology Mono Lake multicellularity Observation Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA California fluorescence in situ hybridization multicellularity Mono Lake evolution bacteria choanoflagellates
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Summary:	As the closest living relatives of animals, choanoflagellates offer insights into the ancestry of animal cell physiology. Here, we report the isolation and characterization of a colonial choanoflagellate from Mono Lake, California. The choanoflagellate forms large spherical colonies that are an order of magnitude larger than those formed by the closely related choanoflagellate . In cultures maintained in the laboratory, the lumen of the spherical colony is filled with a branched network of extracellular matrix and colonized by bacteria, including diverse Gammaproteobacteria and Alphaproteobacteria. We propose to erect gen. nov., sp. nov. Hake, Burkhardt, Richter, and King to accommodate this extremophile choanoflagellate. The physical association between bacteria and in culture presents a new experimental model for investigating interactions among bacteria and eukaryotes. Future work will investigate the nature of these interactions in wild populations and the mechanisms underpinning the colonization of spheres by bacteria. The diversity of organisms that live in the extreme environment of Mono Lake (California, USA) is limited. We sought to investigate whether the closest living relatives of animals, the choanoflagellates, exist in Mono Lake, a hypersaline, alkaline, arsenic-rich environment. We repeatedly isolated members of a new species of choanoflagellate, which we have named . Characterization of revealed that it forms large spherical colonies containing diverse co-isolated bacteria, providing an opportunity to investigate mechanisms underlying physical associations between eukaryotes and bacteria.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: University of Southampton, Southampton, United Kingdom The authors declare no conflict of interest. Present address: Calico Life Sciences, South San Francisco, California, USA
ISSN:	2150-7511 2150-7511
DOI:	10.1128/mbio.01623-24