Search Results - "Laughery, Marian F."

R-loop formation by dCas9 is mutagenic in Saccharomyces cerevisiae by Laughery, Marian F, Mayes, Hannah C, Pedroza, Ivan K, Wyrick, John J

“…Abstract Cas9 binds and cleaves specific DNA sequences by inducing the formation of an R-loop between the guide RNA and its genomic target. While targeting of…”
Get full text

Contributions of replicative and translesion DNA polymerases to mutagenic bypass of canonical and atypical UV photoproducts by Vandenberg, Brittany N., Laughery, Marian F., Cordero, Cameron, Plummer, Dalton, Mitchell, Debra, Kreyenhagen, Jordan, Albaqshi, Fatimah, Brown, Alexander J., Mieczkowski, Piotr A., Wyrick, John J., Roberts, Steven A.

“…UV exposure induces a mutation signature of C > T substitutions at dipyrimidines in skin cancers. We recently identified additional UV-induced AC > TT and A >…”
Get full text

Nucleosomes Selectively Inhibit Cas9 Off-target Activity at a Site Located at the Nucleosome Edge by Hinz, John M., Laughery, Marian F., Wyrick, John J.

“…Nucleosomes affect Cas9 binding and activity at on-target sites, but their impact at off-target sites is unknown. To investigate how nucleosomes affect Cas9…”
Get full text

Atypical UV Photoproducts Induce Non-canonical Mutation Classes Associated with Driver Mutations in Melanoma by Laughery, Marian F., Brown, Alexander J., Bohm, Kaitlynne A., Sivapragasam, Smitha, Morris, Haley S., Tchmola, Mila, Washington, Angelica D., Mitchell, Debra, Mather, Stephen, Malc, Ewa P., Mieczkowski, Piotr A., Roberts, Steven A., Wyrick, John J.

“…Somatic mutations in skin cancers and other ultraviolet (UV)-exposed cells are typified by C>T and CC>TT substitutions at dipyrimidine sequences; however, many…”
Get full text

The Surprising Diversity of UV‐Induced Mutations by Laughery, Marian F., Wilson, Hannah E., Sewell, Allysa, Stevison, Scott, Wyrick, John J.

“…Ultraviolet (UV) light is the most pervasive environmental mutagen and the primary cause of skin cancer. Genome sequencing of melanomas and other skin cancers…”
Get full text

Repair of DNA Double-Strand Breaks following UV Damage in Three Sulfolobus solfataricus Strains by ROLFSMEIER, Michael L, LAUGHERY, Marian F, HASELTINE, Cynthia A

“…Article Usage Stats Services JB Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
Get full text

New vectors for simple and streamlined CRISPR–Cas9 genome editing in Saccharomyces cerevisiae by Laughery, Marian F., Hunter, Tierra, Brown, Alexander, Hoopes, James, Ostbye, Travis, Shumaker, Taven, Wyrick, John J.

“…Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9 technology is an important tool for genome editing because the Cas9 endonuclease can…”
Get full text

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro by Hinz, John M, Laughery, Marian F, Wyrick, John J

“…During Cas9 genome editing in eukaryotic cells, the bacterial Cas9 enzyme cleaves DNA targets within chromatin. To understand how chromatin affects Cas9…”
Get full text

Genome-wide maps of UVA and UVB mutagenesis in yeast reveal distinct causative lesions and mutational strand asymmetries by Laughery, Marian F, Plummer, Dalton A, Wilson, Hannah E, Vandenberg, Brittany N, Mitchell, Debra, Mieczkowski, Piotr A, Roberts, Steven A, Wyrick, John J

“…Abstract Ultraviolet (UV) light primarily causes C > T substitutions in lesion-forming dipyrimidine sequences. However, many of the key driver mutations in…”
Get full text

Site-specific Acetylation of Histone H3 Decreases Polymerase β Activity on Nucleosome Core Particles in Vitro by Rodriguez, Yesenia, Hinz, John M., Laughery, Marian F., Wyrick, John J., Smerdon, Michael J.

“…Histone posttranslational modifications have been associated with changes in chromatin structure necessary for transcription, replication, and DNA repair…”
Get full text

Simple CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae by Laughery, Marian F, Wyrick, John J

“…CRISPR-Cas9 has emerged as a powerful method for editing the genome in a wide variety of species, since it can generate a specific DNA break when targeted by…”
Get more information

Histone Sprocket Arginine Residues Are Important for Gene Expression, DNA Repair, and Cell Viability in Saccharomyces cerevisiae by Hodges, Amelia J, Gallegos, Isaura J, Laughery, Marian F, Meas, Rithy, Tran, Linh, Wyrick, John J

“…A critical feature of the intermolecular contacts that bind DNA to the histone octamer is the series of histone arginine residues that insert into the DNA…”
Get full text

Repair of DNA Double-Strand Breaks Induced by Ionizing Radiation Damage Correlates with Upregulation of Homologous Recombination Genes in Sulfolobus solfataricus by Rolfsmeier, Michael L., Laughery, Marian F., Haseltine, Cynthia A.

“…The mechanisms used by members of the archaeal branch of life to repair DNA damage are not well understood. DNA damage responses have been of particular…”
Get full text

New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae: Vectors for simple CRISPR-Cas9 genome editing in yeast by Laughery, Marian F., Hunter, Tierra, Brown, Alexander, Hoopes, James, Ostbye, Travis, Shumaker, Taven, Wyrick, John J.

Get full text