Search Results - "Lauber, Matthew A"

Assessing the impact of nonspecific binding on oligonucleotide bioanalysis by Nguyen, Jennifer M, Gilar, Martin, Koshel, Brooke, Donegan, Michael, MacLean, Jason, Li, Zhimin, Lauber, Matthew A

“…Accurate and reliable quantification of oligonucleotides can be difficult, which has led to an increased focus on bioanalytical methods for more robust…”
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Quantitative N-Glycan Profiling of Therapeutic Monoclonal Antibodies Performed by Middle-Up Level HILIC-HRMS Analysis by Duivelshof, Bastiaan L., Denorme, Steffy, Sandra, Koen, Liu, Xiaoxiao, Beck, Alain, Lauber, Matthew A., Guillarme, Davy, D’Atri, Valentina

“…The identification and accurate quantitation of the various glycoforms contained in therapeutic monoclonal antibodies (mAbs) is one of the main analytical…”
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Pressure-Enhanced Liquid Chromatography, a Proof of Concept: Tuning Selectivity with Pressure Changes and Gradients by Fekete, Szabolcs, Fogwill, Michael, Lauber, Matthew A.

“…Many chromatographers have observed that the operating pressure can dramatically change the chromatographic retention of solutes. Small molecules show…”
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Theoretical study on solute migration and band broadening occurring in pressure-enhanced liquid chromatography by Fekete, Szabolcs, Lauber, Matthew A.

“…•Migration velocity gradients were studied in pressure enhanced LC (PE-LC).•Band broadening and sharpening in pressurized separations were illustrated.•Both…”
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High sensitivity acidic N-glycan profiling with MS-enhancing derivatization and mixed mode chromatography by Liu, Xiaoxiao, Wang, Qi, Lauber, Matthew A.

“…•A high sensitivity, LC-based profiling technique is established and applied to the analysis of acidic N-glycans of a lysosomal enzyme.•Hybrid silica…”
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Ratiometric Pulse–Chase Amidination Mass Spectrometry as a Probe of Biomolecular Complex Formation by Chang, Feng-Ming James, Lauber, Matthew A, Running, William E, Reilly, James P, Giedroc, David P

“…Selective chemical modification of protein side chains coupled with mass spectrometry is often most informative when used to compare residue-specific…”
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Negative gradient slope methods to improve the separation of closely eluting proteins by Fekete, Szabolcs, Murisier, Amarande, Nguyen, Jennifer M., Lauber, Matthew A., Guillarme, Davy

“…In the present work, we describe the fundamental and practical advantages of a new strategy to improve the resolution of very closely eluting peaks within…”
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The impact of low adsorption surfaces for the analysis of DNA and RNA oligonucleotides by Lardeux, Honorine, Goyon, Alexandre, Zhang, Kelly, Nguyen, Jennifer M, Lauber, Matthew A, Guillarme, Davy, D'Atri, Valentina

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Size Exclusion and Ion Exchange Chromatographic Hardware Modified with a Hydrophilic Hybrid Surface by Fekete, Szabolcs, DeLano, Mathew, Harrison, Andrew Bates, Shiner, Stephen J, Belanger, Jonathan Leon, Wyndham, Kevin D, Lauber, Matthew A

“…Certain biomolecules have proven to be difficult to analyze by liquid chromatography (LC), especially under certain chromatographic conditions. The separation…”
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Towards a simple on-line coupling of ion exchange chromatography and native mass spectrometry for the detailed characterization of monoclonal antibodies by Murisier, Amarande, Duivelshof, Bastiaan L., Fekete, Szabolcs, Bourquin, Julien, Schmudlach, Andrew, Lauber, Matthew A., Nguyen, Jennifer M., Beck, Alain, Guillarme, Davy, D'Atri, Valentina

“…•A simple, easy-to-use CEX-MS method is proposed for mAb analysis.•Standard and narrow bore columns are evaluated in terms of selectivity and…”
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Enhanced Detection of Low-Abundance Host Cell Protein Impurities in High-Purity Monoclonal Antibodies Down to 1 ppm Using Ion Mobility Mass Spectrometry Coupled with Multidimensional Liquid Chromatography by Doneanu, Catalin E, Anderson, Malcolm, Williams, Brad J, Lauber, Matthew A, Chakraborty, Asish, Chen, Weibin

“…The enormous dynamic range of proteinaceous species present in protein biotherapeutics poses a significant challenge for current mass spectrometry (MS)-based…”
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Rapid Preparation of Released N‑Glycans for HILIC Analysis Using a Labeling Reagent that Facilitates Sensitive Fluorescence and ESI-MS Detection by Lauber, Matthew A, Yu, Ying-Qing, Brousmiche, Darryl W, Hua, Zhengmao, Koza, Stephan M, Magnelli, Paula, Guthrie, Ellen, Taron, Christopher H, Fountain, Kenneth J

“…N-glycosylation of proteins is now routinely characterized and monitored because of its significance to the detection of disease states and the manufacturing…”
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Using Hybrid Organic–Inorganic Surface Technology to Mitigate Analyte Interactions with Metal Surfaces in UHPLC by DeLano, Mathew, Walter, Thomas H, Lauber, Matthew A, Gilar, Martin, Jung, Moon Chul, Nguyen, Jennifer M, Boissel, Cheryl, Patel, Amit V, Bates-Harrison, Andrew, Wyndham, Kevin D

“…Interactions of analytes with metal surfaces in high-performance liquid chromatography (HPLC) instruments and columns have been reported to cause deleterious…”
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Ultra-short ion-exchange columns for fast charge variants analysis of therapeutic proteins by Navarro-Huerta, Jose Antonio, Murisier, Amarande, Nguyen, Jennifer M., Lauber, Matthew A., Beck, Alain, Guillarme, Davy, Fekete, Szabolcs

“…•prototype ultra-short ion exchange columns were developed.•the ideal column length was experimentally determined.•elution mechanism of mAbs were studied in pH…”
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High sensitivity LC-MS profiling of antibody-drug conjugates with difluoroacetic acid ion pairing by Nguyen, Jennifer M, Smith, Jacquelynn, Rzewuski, Susan, Legido-Quigley, Cristina, Lauber, Matthew A

“…Reversed-phase liquid chromatography (RPLC) separations of proteins using optical detection generally use trifluoroacetic acid (TFA) because it is a strong,…”
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Use of Ultrashort Columns for Therapeutic Protein Separations. Part 1: Theoretical Considerations and Proof of Concept by Fekete, Szabolcs, Bobály, Balázs, Nguyen, Jennifer M, Beck, Alain, Veuthey, Jean-Luc, Wyndham, Kevin, Lauber, Matthew A, Guillarme, Davy

“…Due to the particular elution mechanism observed with large solutes (e.g., proteins) in liquid chromatography, column length has less impact in controlling…”
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Reducing metal-ion mediated adsorption of acidic peptides in RPLC-based assays using hybrid silica chromatographic surfaces by Birdsall, Robert E., Kellett, Jacob, Ippoliti, Samantha, Ranbaduge, Nilini, Lauber, Matthew A., Yu, Ying Qing, Chen, Weibin

“…•Acidic peptides exhibit metal-induced peak tailing values up to Tf=5.86.•Hybrid silica columns effectively reduce peak tailing (Tf = 5.86 vs. Tf = 1.13) for…”
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Capillary liquid chromatography coupled with mass spectrometry for analysis of nanogram protein quantities on a wide-pore superficially porous particle column in top-down proteomics by Lignieres, Laurent, Legros, Véronique, Khelil, Manel, Senecaut, Nicolas, Lauber, Matthew A, Camadro, Jean-Michel, Chevreux, Guillaume

“…•A wide-pore silica-based superficially porous media with phenyl bonding was evaluated.•DFA is a worth of interest ion pairing agent for…”
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Optimizing test approaches for the detection of exposed metal surfaces within a chromatographic flow path by Simeone, Jennifer, Patel, Amit V., DeLano, Mathew, Shiner, Stephen J., Hoyes, Emmy, Tracey, Francis, Lauber, Matthew A.

“…•Demonstration of analyte adsorption to various metals.•Correlation between analyte recovery and metal surface area within an LC flow path.•Robust test…”
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Use of Ultra-short Columns for Therapeutic Protein Separations, Part 2: Designing the Optimal Column Dimension for Reversed-Phase Liquid Chromatography by Fekete, Szabolcs, Murisier, Amarande, Nguyen, Jennifer M, Bolton, Michael J, Belanger, Jonathan, Beck, Alain, Veuthey, Jean-Luc, Wyndham, Kevin, Lauber, Matthew A, Guillarme, Davy

“…In the first part of the series, it was demonstrated that very fast (<30 s) separations of therapeutic protein species are feasible using ultra-short (5 × 2.1…”
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