Search Results - "Laub, Michael T."

Shutoff of host transcription triggers a toxin-antitoxin system to cleave phage RNA and abort infection by Guegler, Chantal K., Laub, Michael T.

“…Toxin-antitoxin (TA) systems are widespread in bacteria, but their activation mechanisms and bona fide targets remain largely unknown. Here, we characterize a…”
Get full text

Proteotoxic Stress Induces a Cell-Cycle Arrest by Stimulating Lon to Degrade the Replication Initiator DnaA by Jonas, Kristina, Liu, Jing, Chien, Peter, Laub, Michael T.

“…The decision to initiate DNA replication is a critical step in the cell cycle of all organisms. Cells often delay replication in the face of stressful…”
Get full text

Global Analysis of the E. coli Toxin MazF Reveals Widespread Cleavage of mRNA and the Inhibition of rRNA Maturation and Ribosome Biogenesis by Culviner, Peter H., Laub, Michael T.

“…Toxin-antitoxin systems are widely distributed genetic modules that regulate growth and persistence in bacteria. Many systems, including E. coli MazEF, include…”
Get full text

Pervasive degeneracy and epistasis in a protein-protein interface by Podgornaia, Anna I., Laub, Michael T.

“…Mapping protein sequence space is a difficult problem that necessitates the analysis of 20N combinations for sequences of length N. We systematically mapped…”
Get full text

A Bacterial Toxin Inhibits DNA Replication Elongation through a Direct Interaction with the β Sliding Clamp by Aakre, Christopher D., Phung, Tuyen N., Huang, David, Laub, Michael T.

“…Toxin-antitoxin (TA) systems are ubiquitous on bacterial chromosomes, yet the mechanisms regulating their activity and the molecular targets of toxins remain…”
Get full text

ppGpp Coordinates Nucleotide and Amino-Acid Synthesis in E. coli During Starvation by Wang, Boyuan, Grant, Robert A., Laub, Michael T.

“…(p)ppGpp is a nucleotide messenger universally produced in bacteria following nutrient starvation. In E. coli, ppGpp inhibits purine nucleotide synthesis by…”
Get full text

Specificity in two-component signal transduction pathways by Laub, Michael T, Goulian, Mark

“…Two-component signal transduction systems enable bacteria to sense, respond, and adapt to a wide range of environments, stressors, and growth conditions. In…”
Get full text

Bacterial Chromosome Organization and Segregation by Badrinarayanan, Anjana, Le, Tung B.K, Laub, Michael T

“…If fully stretched out, a typical bacterial chromosome would be nearly 1 mm long, approximately 1,000 times the length of a cell. Not only must cells massively…”
Get full text

Transcription rate and transcript length drive formation of chromosomal interaction domain boundaries by Le, Tung BK, Laub, Michael T

“…Chromosomes in all organisms are highly organized and divided into multiple chromosomal interaction domains, or topological domains. Regions of active, high…”
Get full text

High-Resolution Mapping of the Spatial Organization of a Bacterial Chromosome by Le, Tung B. K., Imakaev, Maxim V., Mirny, Leonid A., Laub, Michael T.

“…Chromosomes must be highly compacted and organized within cells, but how this is achieved in vivo remains poorly understood. We report the use of chromosome…”
Get full text

Affinity-based capture and identification of protein effectors of the growth regulator ppGpp by Wang, Boyuan, Dai, Peng, Ding, David, Del Rosario, Amanda, Grant, Robert A., Pentelute, Bradley L., Laub, Michael T.

“…The nucleotide ppGpp is a highly conserved regulatory molecule in bacteria that helps tune growth rate to nutrient availability. Despite decades of study, how…”
Get full text

A Simple, Cost-Effective, and Robust Method for rRNA Depletion in RNA-Sequencing Studies by Culviner, Peter H, Guegler, Chantal K, Laub, Michael T

“…The profiling of gene expression by RNA sequencing (RNA-seq) has enabled powerful studies of global transcriptional patterns in all organisms, including…”
Get full text

Ancestral reconstruction of duplicated signaling proteins reveals the evolution of signaling specificity by Nocedal, Isabel, Laub, Michael T

“…Gene duplication is crucial to generating novel signaling pathways during evolution. However, it remains unclear how the redundant proteins produced by gene…”
Get full text

An interbacterial toxin inhibits target cell growth by synthesizing (p)ppApp by Ahmad, Shehryar, Wang, Boyuan, Walker, Matthew D., Tran, Hiu-Ki R., Stogios, Peter J., Savchenko, Alexei, Grant, Robert A., McArthur, Andrew G., Laub, Michael T., Whitney, John C.

“…Bacteria have evolved sophisticated mechanisms to inhibit the growth of competitors 1 . One such mechanism involves type VI secretion systems, which bacteria…”
Get full text

Condensin promotes the juxtaposition of DNA flanking its loading site in Bacillus subtilis by Wang, Xindan, Le, Tung B K, Lajoie, Bryan R, Dekker, Job, Laub, Michael T, Rudner, David Z

“…SMC condensin complexes play a central role in compacting and resolving replicated chromosomes in virtually all organisms, yet how they accomplish this remains…”
Get full text

SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription by Tran, Ngat T., Laub, Michael T., Le, Tung B.K.

“…The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In…”
Get full text

Regulatory Cohesion of Cell Cycle and Cell Differentiation through Interlinked Phosphorylation and Second Messenger Networks by Abel, Sören, Chien, Peter, Wassmann, Paul, Schirmer, Tilman, Kaever, Volkhard, Laub, Michael T., Baker, Tania A., Jenal, Urs

“…In Caulobacter crescentus, phosphorylation of key regulators is coordinated with the second messenger cyclic di-GMP to drive cell-cycle progression and…”
Get full text

The evolution of a counter-defense mechanism in a virus constrains its host range by Srikant, Sriram, Guegler, Chantal K, Laub, Michael T

“…Bacteria use diverse immunity mechanisms to defend themselves against their viral predators, bacteriophages. In turn, phages can acquire counter-defense…”
Get full text

Temporal and evolutionary dynamics of two-component signaling pathways by Salazar, Michael E, Laub, Michael T

“…Graphical abstract…”
Get full text

Polarity and cell fate asymmetry in Caulobacter crescentus by Tsokos, Christos G, Laub, Michael T

“…Highlights ► Replicative asymmetry of Caulobacter daughter cells is dictated by CtrA. ► Two phosphorelays drive the phosphorylation and proteolytic…”
Get full text