DNA immobilization on modified inorganic sorbents

Fragments of denaturated DNA having the length of about 500 nucleotides were immobilized, using silica carriers modified by organic polymers. It was shown that the organic coating allows to obtain sorbents, which practically exclude non-specific sorption with respect to DNA. The immobilization was c...

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Bibliographic Details
Published in:	Biokhimiia (Moscow, Russia) Vol. 45; no. 9; p. 1597
Main Authors:	Latich, V I, Varlamov, V P, Semenova, N N, Rogozhin, S V
Format:	Journal Article
Language:	Russian
Published:	Russia (Federation) 01-09-1980
Subjects:	Adsorption Animals Chemical Phenomena Chemistry Deoxyribonucleases DNA DNA Restriction Enzymes Endonucleases Isoenzymes Nucleic Acid Denaturation Pancreas - enzymology Phosphodiesterase I Phosphoric Diester Hydrolases Polymers Structure-Activity Relationship
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Summary:	Fragments of denaturated DNA having the length of about 500 nucleotides were immobilized, using silica carriers modified by organic polymers. It was shown that the organic coating allows to obtain sorbents, which practically exclude non-specific sorption with respect to DNA. The immobilization was carried out on hydroxyl-containing sorbents and on their phrosphorylated derivatives by means of water-soluble carbodiimide. The resulting preparations contained up to 60 units at A260 of DNA per 1 g of carrier. The effects of endonuclease A236, exonuclease A5 and pancreatic DNAse on the immobilized RNA were studied.
ISSN:	0320-9725