Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver

Stigmasterol isolated from the chloroform fraction of Adenopus breviflorus Benth fruit induces mitochondrial-dependent apoptosis in rat liver

Decoction of fruit is used in folkloric medicine for treating dysmenorrhea and gonorrhea. Phytochemicals from may be potent in inducing mitochondrial-dependent apoptosis via the opening of the mitochondrial permeability transition (MPT) pore. Therefore, this study investigated the effects of stigmas...

Full description

Saved in:
Bibliographic Details
Published in:Journal of complementary & integrative medicine Vol. 18; no. 4; p. 737
Main Authors: Oyedeji, Tolulope A, Onireti, Daniel O, Lasisi, Olaitan S, Akobi, Chibuzor I, Olorunsogo, Olufunso O
Format: Journal Article
Language:English
Published: Germany 10-05-2021
Subjects:
Animals
Apoptosis
Calcium
Chloroform
Fruit
Liver
Rats
Rats, Wistar
Stigmasterol - pharmacology
Adenopus breviflorus
apoptosis
cytochrome c release
stigmasterol
mitochondrial permeability transition pore
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Decoction of fruit is used in folkloric medicine for treating dysmenorrhea and gonorrhea. Phytochemicals from may be potent in inducing mitochondrial-dependent apoptosis via the opening of the mitochondrial permeability transition (MPT) pore. Therefore, this study investigated the effects of stigmasterol isolated from the chloroform fraction of (CFAB) and also the increasing concentration of CFAB on the opening of rat liver mitochondrial permeability transition (MPT) pore. Fractionation of CFAB on column chromatography yielded a needle-like crystal which structure was elucidated by standard spectroscopic techniques. The effects of stigmasterol and CFAB on MPT pore opening were assayed spectrophotometrically. Also, the effect of CFAB on mitochondrial ATPase (mATPase) activity and cytochrome c (Cyt c) release were determined. Stigmasterol isolated from CFAB induced MPT pore opening significantly (p<0.05) when compared with the control. Similarly, CFAB significantly (p<0.05) induced MPT pore opening in rat liver mitochondria in a concentration-dependent manner in the presence and absence of the triggering agent - calcium ion. Furthermore, the increasing concentration of CFAB significantly (p<0.05) stimulated mitochondrial ATPase (mATPase) activity and Cyt c release in a concentration-dependent manner. The study showed that stigmasterol isolated from the chloroform fraction of is a potent inducer of mitochondrial-dependent apoptosis. Also, the study further revealed that CFAB possesses potent bioactive compounds which can induce the mitochondrial-dependent apoptosis through the opening of the mitochondrial permeability transition pore, activation of mitochondrial ATPase (mATPase) activity and cytochrome c release.
ISSN:1553-3840
DOI:10.1515/jcim-2020-0323