Search Results - "Lario, Paula I."

The bacterial virulence factor NleA inhibits cellular protein secretion by disrupting mammalian COPII function by Kim, Jinoh, Thanabalasuriar, Ajitha, Chaworth-Musters, Tessa, Fromme, J Chris, Frey, Elizabeth A, Lario, Paula I, Metalnikov, Pavel, Rizg, Keyrillos, Thomas, Nikhil A, Lee, Sau Fung, Hartland, Elizabeth L, Hardwidge, Philip R, Pawson, Tony, Strynadka, Natalie C, Finlay, B Brett, Schekman, Randy, Gruenheid, Samantha

“…Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) maintain an extracellular lifestyle and use a type III secretion system to translocate…”
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Sub-atomic Resolution Crystal Structure of Cholesterol Oxidase: What Atomic Resolution Crystallography Reveals about Enzyme Mechanism and the Role of the FAD Cofactor in Redox Activity by Lario, Paula I., Sampson, Nicole, Vrielink, Alice

“…The crystal structure of cholesterol oxidase, a 56kDa flavoenzyme was anisotropically refined to 0.95Å resolution. The final crystallographic R-factor and…”
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Structural Characterization of the Type-III Pilot-Secretin Complex from Shigella flexneri by Okon, Mark, Moraes, Trevor F., Lario, Paula I., Creagh, A. Louise, Haynes, Charles A., Strynadka, Natalie C.J., McIntosh, Lawrence P.

“…Assembly of the type-III secretion apparatus, which translocates proteins through both membranes of Gram-negative bacterial pathogens into host cells, requires…”
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Structure and biochemical analysis of a secretin pilot protein by Lario, Paula I, Pfuetzner, Richard A, Frey, Elizabeth A, Creagh, Louise, Haynes, Charles, Maurelli, Anthony T, Strynadka, Natalie C J

“…The ability to translocate virulence proteins into host cells through a type III secretion apparatus (TTSS) is a hallmark of several Gram‐negative pathogens…”
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Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment by Moustafa, Ibrahim, Vrielink, Alice, Lario, Paula I, Lyubimov, Artem Y

“…Hydrogen atoms are a vital component of enzyme structure and function. In recent years, atomic resolution crystallography (≥1.2 Å) has been successfully used…”
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Improving biophysical properties of a bispecific antibody scaffold to aid developability: quality by molecular design by Von Kreudenstein, Thomas Spreter, Escobar-Carbrera, Eric, Lario, Paula I, D'Angelo, Igor, Brault, Karine, Kelly, John, Durocher, Yves, Baardsnes, Jason, Woods, R Jeremy, Xie, Michael Hongwei, Girod, Pierre-Alain, Suits, Michael D L, Boulanger, Martin J, Poon, David K Y, Ng, Gordon Y K, Dixit, Surjit B

“…While the concept of Quality-by-Design is addressed at the upstream and downstream process development stages, we questioned whether there are advantages to…”
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Protein engineering and the use of molecular modeling and simulation: The case of heterodimeric Fc engineering by Spreter Von Kreudenstein, Thomas, Lario, Paula I., Dixit, Surjit B.

“…Computational and structure guided methods can make significant contributions to the development of solutions for difficult protein engineering problems,…”
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Atomic Resolution Density Maps Reveal Secondary Structure Dependent Differences in Electronic Distribution by Lario, Paula I, Vrielink, Alice

“…The X-ray crystal structure of the flavoenzyme cholesterol oxidase, SCOA (Streptomyces sp.SA-COO) has been determined to 0.95 Å resolution. The large size…”
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Erratum: Atomic resolution crystallography reveals how changes in pH shape the protein microenvironment by Lyubimov, Artem Y, Lario, Paula I, Moustafa, Ibrahim, Vrielink, Alice

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The Presence of a Hydrogen Bond between Asparagine 485 and the π System of FAD Modulates the Redox Potential in the Reaction Catalyzed by Cholesterol Oxidase by Yin, Ye, Sampson, Nicole S, Vrielink, Alice, Lario, Paula I

“…Cholesterol oxidase catalyzes the oxidation and isomerization of cholesterol to cholest-4-en-3-one. An asparagine residue (Asn485) at the active site is…”
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Purification and Characterization of the Human PDE4A Catalytic Domain (PDE4A330–723) Expressed in Sf9 Cells by Lario, Paula I., Bobechko, Brian, Bateman, Kevin, Kelly, John, Vrielink, Alice, Huang, Zheng

“…The human PDE4A catalytic domain (PDE4A330–723) expressed in Sf9 cells was found to be heavily phosphorylated on both serines of the conserved SPS motif by…”
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Towards understanding flavin reactivity: A structural study of cholesterol oxidase by Lario, Paula I

“…Flavoenzymes catalyze a wide variety of biochemical reactions and are commonly observed as electron transport proteins. The redox reactive portion of the…”
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Improving biophysical properties of a bispecific antibody scaffold to aid developability: Quality by molecular design by Von Kreudenstein, Thomas Spreter, Escobar-Carbrera, Eric, Lario, Paula I., D'Angelo, Igor, Brault, Karine, Kelly, John F, Durocher, Yves, Baardsnes, Jason, Woods, R. Jeremy, Xie, Michael Hongwei, Girod, Pierre-Alain, Suits, Michael D. L., Boulanger, Martin J., Poon, David K. Y., Ng, Gordon Y., Dixit, Surjit B.

“…While the concept of Quality-by-Design is addressed at the upstream and downstream process development stages, we questioned whether there are advantages to…”
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