Search Results - "Lapujade, P"
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When transcriptome meets metabolome: fast cellular responses of yeast to sudden relief of glucose limitation
Published in Molecular systems biology (2006)“…Within the first 5 min after a sudden relief from glucose limitation, Saccharomyces cerevisiae exhibited fast changes of intracellular metabolite levels and a…”
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De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology
Published in Microbial cell factories (26-03-2012)“…Saccharomyces cerevisiae CEN.PK 113-7D is widely used for metabolic engineering and systems biology research in industry and academia. We sequenced, assembled,…”
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Energetic limits to metabolic flexibility: responses of Saccharomyces cerevisiae to glucose-galactose transitions
Published in Microbiology (Society for General Microbiology) (01-04-2009)“…1 Kluyver Centre for Genomics of Industrial Fermentation and Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The…”
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A proteome-integrated, carbon source dependent genetic regulatory network in Saccharomyces cerevisiae
Published in Molecular omics (17-02-2020)“…Integrated regulatory networks can be powerful tools to examine and test properties of cellular systems, such as modelling environmental effects on the…”
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Glutamate biosynthesis in Lactococcus lactis subsp. lactis NCDO 2118
Published in Applied and Environmental Microbiology (01-07-1998)“…Unlike other lactic acid bacteria, Lactococcus lactis subsp. lactis NCDO 2118 was able to grow in a medium lacking glutamate and the amino acids of the…”
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An improved temperature-triggered process for glutamate production with Corynebacterium glutamicum
Published in Enzyme and microbial technology (01-11-1999)“…An improved glutamate-producing fed-batch process, using a temperature-sensitive strain of Corynebacterium glutamicum, has been characterized. By a tight…”
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Glutamate as an inhibitor of phosphoenolpyruvate carboxylase activity in Corynebacterium glutamicum
Published in Journal of industrial microbiology & biotechnology (01-05-2004)“…The glutamate-producing bacterium, Corynebacterium glutamicum is known to possess two anaplerotic enzymes: pyruvate carboxylase (Pc) and phosphoenolpyruvate…”
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Flexibility of the metabolism of Corynebacterium glutamicum 2262, a glutamic acid-producing bacterium, in response to temperature upshocks
Published in Journal of industrial microbiology & biotechnology (01-06-2002)“…: In order to test the temperature sensitivity of glutamate production metabolism, several temperature shifts, from 33 to 37, 38, 39, 40 or 41°C, were applied…”
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Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies
Published in Nature communications (10-02-2022)“…When conditions change, unicellular organisms rewire their metabolism to sustain cell maintenance and cellular growth. Such rewiring may be understood as…”
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Objective quantification of plaque using digital image analysis
Published in Monographs in oral science (2000)“…Dental plaque is the precursor to many oral diseases (e.g. gingivitis, periodontitis, caries) and thus its removal and control are an important aspect of oral…”
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Transcriptome-Based Characterization of Interactions between Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus in Lactose-Grown Chemostat Cocultures
Published in Applied and Environmental Microbiology (01-10-2013)“…Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit…”
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Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains
Published in Nature communications (01-12-2010)“…The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook…”
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Measuring enzyme activities under standardized in vivo-like conditions for systems biology
Published in The FEBS journal (01-02-2010)“…Realistic quantitative models require data from many laboratories. Therefore, standardization of experimental systems and assay conditions is crucial…”
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Role of transcriptional regulation in controlling fluxes in central carbon metabolism of Saccharomyces cerevisiae. A chemostat culture study
Published in The Journal of biological chemistry (05-03-2004)“…In contrast to batch cultivation, chemostat cultivation allows the identification of carbon source responses without interference by carbon-catabolite…”
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Reproducibility of oligonucleotide microarray transcriptome analyses. An interlaboratory comparison using chemostat cultures of Saccharomyces cerevisiae
Published in The Journal of biological chemistry (04-10-2002)“…Assessment of reproducibility of DNA-microarray analysis from published data sets is complicated by the use of different microbial strains, cultivation…”
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Two-dimensional transcriptome analysis in chemostat cultures. Combinatorial effects of oxygen availability and macronutrient limitation in Saccharomyces cerevisiae
Published in The Journal of biological chemistry (07-01-2005)“…Genome-wide analysis of transcriptional regulation is generally studied by determining sets of "signature transcripts" that are up- or down-regulated relative…”
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Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity
Published in Microbiology (Society for General Microbiology) (01-05-2005)“…1 Kluyver Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands 2 DSM Life Sciences, Bakery Ingredients…”
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Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae
Published in Microbial cell (21-10-2015)“…Stationary-phase (SP) batch cultures of , in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan,…”
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Prolonged maltose-limited cultivation of Saccharomyces cerevisiae selects for cells with improved maltose affinity and hypersensitivity
Published in Applied and Environmental Microbiology (01-04-2004)“…Prolonged cultivation (>25 generations) of Saccharomyces cerevisiae in aerobic, maltose-limited chemostat cultures led to profound physiological changes…”
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Comparative genotyping of the Saccharomyces cerevisiae laboratory strains S288C and CEN.PK113-7D using oligonucleotide microarrays
Published in FEMS yeast research (01-12-2003)“…To analyse the reliability and accuracy of genotype analysis with high-density oligonucleotide microarrays, this method and other experimental approaches were…”
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