Search Results - "Lao, Nga T"

Continuous translation of circularized mRNA improves recombinant protein titer by Costello, Alan, Lao, Nga T., Barron, Niall, Clynes, Martin

“…Recent success in demonstrating the translation of circular RNA open reading frames or circular mRNA, may offer a new avenue for improving recombinant protein…”
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A proteomic profiling dataset of recombinant Chinese hamster ovary cells showing enhanced cellular growth following miR-378 depletion by Coleman, Orla, Costello, Alan, Henry, Michael, Lao, Nga T., Barron, Niall, Clynes, Martin, Meleady, Paula

“…The proteomic data presented in this article provide supporting information to the related research article "Depletion of endogenous miRNA-378-3p increases…”
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Reinventing the Wheel: Synthetic Circular RNAs for Mammalian Cell Engineering by Costello, Alan, Lao, Nga T., Barron, Niall, Clynes, Martin

“…The circular RNA renaissance is upon us. Recent reports demonstrate applications of synthetic circular RNA molecules as gene therapies and in the production of…”
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CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells by Kellner, Kevin, Lao, Nga T, Barron, Niall

“…MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of potentially…”
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Investigation and circumvention of transfection inhibition by ferric ammonium citrate in serum‐free media for Chinese hamster ovary cells by Capella Roca, Berta, Lao, Nga T., Clynes, Martin, Doolan, Padraig

“…While reliable transfection methods are essential for Chinese hamster ovary (CHO) cell line engineering, reduced transfection efficiencies have been observed…”
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Depletion of endogenous miRNA-378-3p increases peak cell density of CHO DP12 cells and is correlated with elevated levels of ubiquitin carboxyl-terminal hydrolase 14 by Costello, Alan, Coleman, Orla, Lao, Nga T., Henry, Michael, Meleady, Paula, Barron, Niall, Clynes, Martin

“…•Stable depletion of six miRNAs previously associated with cell specific growth rate were functionally validated in CHO DP12 cells.•Depletion of endogenous…”
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Regulation of multiple aquaporin genes in Arabidopsis by a pair of recently duplicated DREB transcription factors by Rae, Liina, Lao, Nga T., Kavanagh, Tony A.

“…Identifying the transcription factors that mediate responses to abiotic stress is of fundamental importance in plant biology, not least because of their…”
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Versatile dual reporter gene systems for investigating stop codon readthrough in plants by Lao, Nga T, Maloney, Alan P, Atkins, John F, Kavanagh, Tony A

“…Translation is most often terminated when a ribosome encounters the first in-frame stop codon (UAA, UAG or UGA) in an mRNA. However, many viruses (and some…”
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Improved yield of rhEPO in CHO cells with synthetic 5′ UTR by Costello, Alan, Lao, Nga T., Barron, Niall, Clynes, Martin

“…The impact of local structure on mRNA translation is not well-defined pertaining to the 5′ UTR. Reports suggest structural remodelling of the 5′ UTR can…”
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Leaky Expression of the TET‐On System Hinders Control of Endogenous miRNA Abundance by Costello, Alan, Lao, Nga T., Gallagher, Clair, Capella Roca, Berta, Julius, Lourdes A.N., Suda, Srinivas, Ducrée, Jens, King, Damien, Wagner, Roland, Barron, Niall, Clynes, Martin

“…With the ability to affect multiple genes and fundamental pathways simultaneously, miRNA engineering of Chinese Hamster Ovary (CHO) cells has significant…”
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CHO cell culture longevity and recombinant protein yield are enhanced by depletion of miR-7 activity via sponge decoy vectors by Sanchez, Noelia, Kelly, Paul, Gallagher, Clair, Lao, Nga T., Clarke, Colin, Clynes, Martin, Barron, Niall

“…Improving the efficiency of recombinant protein production by CHO cells is highly desirable as more complex proteins (MAbs, fusion proteins, blood/clotting…”
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Re-programming CHO cell metabolism using miR-23 tips the balance towards a highly productive phenotype by Kelly, Paul S., Breen, Laura, Gallagher, Clair, Kelly, Shane, Henry, Michael, Lao, Nga T., Meleady, Paula, O'Gorman, Donal, Clynes, Martin, Barron, Niall

“…microRNA engineering of CHO cells has already proved successful in enhancing various industrially relevant phenotypes and producing various recombinant…”
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Manipulating MiRNA Expression to Uncover Hidden Functions by Aherne, Sinéad T, Lao, Nga T

“…There are a numerous target prediction algorithms that allow users to identify putative targets of their microRNAs (miRNAs) of interest. Although these tools…”
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miR‐CATCH Identifies Biologically Active miRNA Regulators of the Pro‐Survival Gene XIAP, in Chinese Hamster Ovary Cells by Griffith, Alan, Kelly, Paul S., Vencken, Sebastian, Lao, Nga T., Greene, Catherine M., Clynes, Martin, Barron, Niall

“…Genetic engineering of mammalian cells is of interest as a means to boost bio‐therapeutic protein yield. X‐linked inhibitor of apoptosis (XIAP) overexpression…”
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nonlethal sampling method to obtain, generate and assemble whole blood transcriptomes from small, wild mammals by Huang, Zixia, Gallot, Aurore, Lao, Nga T., Puechmaille, Sébastien J., Foley, Nicole M., Jebb, David, Bekaert, Michaël, Teeling, Emma C.

“…The acquisition of tissue samples from wild populations is a constant challenge in conservation biology, especially for endangered species and protected…”
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Many Parallel Losses of infA from Chloroplast DNA during Angiosperm Evolution with Multiple Independent Transfers to the Nucleus by Millen, Ronny S., Olmstead, Richard G., Adams, Keith L., Palmer, Jeffrey D., Lao, Nga T., Heggie, Laura, Kavanagh, Tony A., Hibberd, Julian M., Gray, John C., Morden, Clifford W., Calie, Patrick J., Jermiin, Lars S., Wolfe, Kenneth H.

“…We used DNA sequencing and gel blot surveys to assess the integrity of the chloroplast gene infA, which codes for translation initiation factor 1, in >300…”
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Gene duplication, exon gain and neofunctionalization of OEP16‐related genes in land plants by Drea, Sinéad C., Lao, Nga T., Wolfe, Kenneth H., Kavanagh, Tony A.

“…Summary OEP16, a channel protein of the outer membrane of chloroplasts, has been implicated in amino acid transport and in the substrate‐dependent import of…”
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Intron-regulated expression of SUVH3, an Arabidopsis Su(var)3-9 homologue by Casas-Mollano, Juan A., Lao, Nga T., Kavanagh, Tony A.

“…SU(VAR)3-9 proteins are key regulators of heterochromatin structure and function in plants, mammals, Drosophila, and yeast. In contrast to animals and fungi,…”
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Mutation of a family 8 glycosyltransferase gene alters cell wall carbohydrate composition and causes a humidity-sensitive semi-sterile dwarf phenotype in Arabidopsis by Lao, Nga T, Long, Debbie, Kiang, Sophie, Coupland, George, Shoue, Douglas A, Carpita, Nicholas C, Kavanagh, Tony A

“…The genome of Arabidopsis thaliana contains about 400 genes coding for glycosyltransferases, many of which are predicted to be involved in the synthesis and…”
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Identification of imprinted genes subject to parent-of-origin specific expression in Arabidopsis thaliana seeds by McKeown, Peter C, Laouielle-Duprat, Sylvia, Prins, Pjotr, Wolff, Philip, Schmid, Marc W, Donoghue, Mark T A, Fort, Antoine, Duszynska, Dorota, Comte, Aurélie, Lao, Nga Thi, Wennblom, Trevor J, Smant, Geert, Köhler, Claudia, Grossniklaus, Ueli, Spillane, Charles

“…Epigenetic regulation of gene dosage by genomic imprinting of some autosomal genes facilitates normal reproductive development in both mammals and flowering…”
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