Search Results - "Lanzetti, AJ"

Spontaneous alpha-N-6-phosphogluconoylation of a "His tag" in Escherichia coli: the cause of extra mass of 258 or 178 Da in fusion proteins by Geoghegan, K F, Dixon, H B, Rosner, P J, Hoth, L R, Lanzetti, A J, Borzilleri, K A, Marr, E S, Pezzullo, L H, Martin, L B, LeMotte, P K, McColl, A S, Kamath, A V, Stroh, J G

“…Several proteins expressed in Escherichia coli with the N-terminus Gly-Ser-Ser-[His]6- consisted partly (up to 20%) of material with 178 Da of excess mass,…”
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LC-mass spectrometry analysis of N- and C-terminal boundary sequences of polypeptide fragments by limited proteolysis by Stroh, Justin G., Loulakis, Pat, Lanzetti, Anthony J., Xie, Julie

“…Limited proteolysis is an important and widely used method for analyzing the tertiary structure and determining the domain boundaries of proteins. Here we…”
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Purification and characterisation of a serine peptidase from the marine psychrophile strain PA-43 by Irwin, Jane A, Alfredsson, Gudni A, Lanzetti, Anthony J, Gudmundsson, Haflidi M, Engel, Paul C

“…An extracellular serine peptidase, purified from the culture supernatant of the sub-Arctic psychrophilic bacterium strain PA-43, is monomeric, with a relative…”
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IL-1 beta maturation: evidence that mature cytokine formation can be induced specifically by nigericin by Perregaux, D, Barberia, J, Lanzetti, AJ, Geoghegan, KF, Carty, TJ, Gabel, CA

“…Mouse peritoneal macrophages stimulated with LPS produce large amounts of pro-IL-1 beta. When these cells were pulse-labeled with [35S]methionine, however,…”
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Mutation of a Protease-sensitive Region in Firefly Luciferase Alters Light Emission Properties by Thompson, John F., Geoghegan, Kieran F., Lloyd, David B., Lanzetti, Anthony J., Magyar, Rachelle A., Anderson, Shannon M., Branchini, Bruce R.

“…Luciferase (EC 1.13.12.7) from the North American firefly, Photinus pyralis, is widely used as a reporter enzyme in cell biology. One of its distinctive…”
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Characterization of alanine and malate dehydrogenases from a marine psychrophile strain PA-43 by IRWIN, Jane A, GUDMUNDSSON, Haflidi M, MARTEINSSON, Viggo T, HREGGVIDSSON, Gudmundur O, LANZETTI, Anthony J, ALFREDSSON, Gudni A, ENGEL, Paul C

“…Alanine dehydrogenase (AlaDH: EC 1.4.1.1), malate dehydrogenase (MDH: EC 1.1.1.37), and glutamate dehydrogenase (EC 1.4.1.2), all NAD+ dependent, were detected…”
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Comparison of the N-terminal sequence of NADH oxidase from Thermus aquaticus with those of other flavoenzymes by Toomey, D, Mayhew, S G, Lanzetti, A J, Geoghegan, K F

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Identification of the 14 kDa bile acid transport protein of rat ileal cytosol as gastrotropin by Vodenlich, Jr, A D, Gong, Y Z, Geoghegan, K F, Lin, M C, Lanzetti, A J, Wilson, F A

“…The 14 kDa bile acid binding protein of rat ileal cytosol (I-BABP), previously shown to be the major intracellular transporter of bile acids in enterocytes,…”
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Functional consequences of posttranslational isomerization of Ser46 in a calcium channel toxin by HECK, S. D, SIOK, C. J, GRAY, W. R, PHILLIPS, D, PARKS, T. N, JACKSON, H, AHLIJANIAN, M. K, SACCOMANO, N. A, VOLKMANN, R. A, KRAPCHO, K. J, KELBAUGH, P. R, THADEIO, P. F, WELCH, M. J, WILLIAMS, R. D, GANONG, A. H, KELLY, M. E, LANZETTI, A. J

“…The venom of the funnel-web spider Agelenopsis aperta contains several peptides that paralyze prey by blocking voltage-sensitive calcium channels. Two…”
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Identification and characterization of a C-terminally extended form of recombinant murine IL-6 by Danley, Dennis E., Strick, Christine A., James, Larry C., Lanzetti, Anthony J., Otterness, Ivan G., Grenett, Hernan E., Fuller, Gerald M.

“…Murine interleukin-6 (mIL-6) was expressed in Escherichia coli in the insoluble fraction of cell lysates. Approximately equal amounts of two polypeptide…”
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Crystallization and preliminary X-ray analysis of complexes of peptide inhibitors with human recombinant and mouse submandibular renins by Badasso, M, Frazao, C, Sibanda, B L, Dhanaraj, V, DeAlwis, C, Cooper, J B, Wood, S P, Blundell, T L, Murakami, K, Miyazaki, H

“…Inhibitor-complexed crystals of mouse and human renins suitable for X-ray analysis have been prepared. The mouse renin is complexed with a non-hydrolysable…”
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