Search Results - "Lanza, Daniel C. F."
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Design and in silico validation of polymerase chain reaction primers to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Published in Scientific reports (15-06-2021)“…Accurate designing of polymerase chain reaction (PCR) primers targeting conserved segments in viral genomes is desirable for preventing false-negative results…”
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2A and 2A-like Sequences: Distribution in Different Virus Species and Applications in Biotechnology
Published in Viruses (26-10-2021)“…2A is an oligopeptide sequence that mediates a ribosome "skipping" effect and can mediate a co-translation cleavage of polyproteins. These sequences are widely…”
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FEZ1 interacts with CLASP2 and NEK1 through coiled-coil regions and their cellular colocalization suggests centrosomal functions and regulation by PKC
Published in Molecular and cellular biochemistry (01-05-2010)“…FEZ1 was initially described as a neuronal protein that influences axonal development and cell polarization. CLASP2 and NEK1 proteins are present in a…”
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New insights about ORF1 coding regions support the proposition of a new genus comprising arthropod viruses in the family Totiviridae
Published in Virus research (04-01-2016)“…•Arthropod totiviruses have peculiar characteristics.•At least two additional coding regions are present in ORF1.•Two exclusive additional proteins can mediate…”
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Analysis of new isolates reveals new genome organization and a hypervariable region in infectious myonecrosis virus (IMNV)
Published in Virus research (04-05-2015)“…•Additional sequences revealed that IMNV genome has at least 8226bp and not 7561bp.•These additional sequences affect the sizes predicted for 5′ UTR, 3′ UTR…”
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Human FEZ1 has characteristics of a natively unfolded protein and dimerizes in solution
Published in Proteins, structure, function, and bioinformatics (01-01-2009)“…The fasciculation and elongation protein Zeta 1 (FEZ1) is the mammalian orthologue of the Caenorhabditis elegans protein UNC‐76, which is necessary for axon…”
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Infectious hypodermal and hematopoietic necrosis virus from Brazil: Sequencing, comparative analysis and PCR detection
Published in Virus research (30-08-2014)“…•A 3739 nucleotide fragment of a Brazilian IHHNV detected in Litopenaeus vannamei was sequenced.•Brazilian IHHNV is very similar to Hawaii isolate, and belongs…”
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"In-House" Production of Single Stranded Oligodeoxyribonucleotides
Published in Nucleic acid therapeutics (01-04-2017)“…The most widely used technique for the production of DNA aptamers/oligonucleotides is chemical synthesis. Despite its effectiveness, this technique cannot be…”
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In silico single strand melting curve: a new approach to identify nucleic acid polymorphisms in Totiviridae
Published in BMC bioinformatics (16-07-2014)“…The PCR technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution…”
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10
Pathogenicity and phenotypic sulfadiazine resistance of Toxoplasma gondii isolates obtained from livestock in northeastern Brazil
Published in Memórias do Instituto Oswaldo Cruz (01-06-2016)“…Toxoplasma gondii is the causative protozoan agent of toxoplasmosis, which is a common infection that is widely distributed worldwide. Studies revealed…”
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Evolutionary origin of 2A-like sequences in Totiviridae genomes
Published in Virus research (02-01-2019)“…•A new phylogenetic reconstruction of the Totiviridae family is presented.•The expanded Giardiavirus clade were characterized.•Sequences resembling 2A-like…”
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Over-expression of GFP-FEZ1 causes generation of multi-lobulated nuclei mediated by microtubules in HEK293 cells
Published in Experimental cell research (10-06-2008)“…FEZ1 (Fasciculation and elongation protein zeta 1) is an ortholog of the Caenorhabditis elegans protein UNC-76, involved in neuronal development and axon…”
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13
Alternative primers to identify a range of apicomplexan parasites
Published in Journal of microbiological methods (01-08-2020)“…Here we present an alternative polymerase chain reaction (PCR) approach using 18S rDNA to identify apicomplexan parasites. A new primer set was designed and…”
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14
A seminested PCR assay for detection and typing of human papillomavirus based on E1 gene sequences
Published in Diagnostic microbiology and infectious disease (01-05-2018)“…HPV infection is considered one of the leading causes of cervical cancer in the world. To date, more than 180 types of HPV have been described and viral typing…”
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Alternative PCR primers for genotyping of Brazilian WSSV isolates
Published in Journal of invertebrate pathology (01-03-2019)“…[Display omitted] •New primers for WSSV genotyping were designed.•New primers were more efficient than previous primers in a pilot epidemiological study.•The…”
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16
An alternative nested-PCR assay for the detection of Toxoplasma gondii strains based on GRA7 gene sequences
Published in Acta tropica (01-07-2016)“…[Display omitted] A nested-PCR assay designed to detect Toxoplasma gondii using the GRA7 gene as a target showed great sensitivity and specificity, and was…”
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Improved reverse transcription-polymerase chain reaction assay for the detection of flaviviruses with semi-nested primers for discrimination between dengue virus serotypes and Zika virus
Published in Memórias do Instituto Oswaldo Cruz (01-01-2018)“…BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most…”
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Biology and Natural History of Type 1 Diabetes Mellitus
Published in Current pediatric reviews (01-08-2023)“…Type 1 diabetes mellitus is a clinical condition characterized by insufficient insulin production due to progressive loss of pancreatic islet β-cells mediated…”
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Pathogenicity and phenotypic sulfadiazine resistance ofToxoplasma gondii isolates obtained from livestock in northeastern Brazil
Published in Memórias do Instituto Oswaldo Cruz (01-06-2016)“…Toxoplasma gondii is the causative protozoan agent of toxoplasmosis, which is a common infection that is widely distributed worldwide. Studies revealed…”
Get full text
Journal Article -
20
In silicosingle strand melting curve: a new approach to identify nucleic acid polymorphisms in Totiviridae
Published in BMC bioinformatics (16-07-2014)“…The PCR technique and its variations have been increasingly used in the clinical laboratory and recent advances in this field generated new higher resolution…”
Get full text
Journal Article