Modulation of AMP Deaminase in Rat Hearts Subjected to Ischemia and Reperfusion by Purine Riboside

Modulation of AMP Deaminase in Rat Hearts Subjected to Ischemia and Reperfusion by Purine Riboside

Changes in AMP deaminase (AMPD) activity influence heart function and progression of heart disease, but the underlying mechanism is unknown. We evaluated the effect of purine riboside (Purr) on the activity of AMPD in perfused rat hearts and in isolated rat cardiomyocytes. Brief perfusion of the pre...

Full description

Saved in:
Bibliographic Details
Published in:Nucleosides, nucleotides & nucleic acids Vol. 27; no. 6-7; pp. 876 - 880
Main Authors: Borkowski, T., Lipinski, M., Kaminski, R., Krzyminska-Stasiuk, E., Langowska, M., Raczak, G., Slominska, E. M., Smolenski, R. T.
Format: Journal Article
Language:English
Published: United States Taylor & Francis Group 01-06-2008
Subjects:
AMP deaminase
AMP Deaminase - antagonists & inhibitors
AMP Deaminase - metabolism
Animals
cardiomyocytes
Enzyme Activation - drug effects
heart
Heart - drug effects
Ischemia - metabolism
ischemia-reperfusion
Myocardium - enzymology
Myocardium - pathology
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - metabolism
Nucleotides - metabolism
Purine Nucleosides - pharmacology
purine riboside
rat
Rats
Reperfusion
Ribonucleosides - pharmacology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Changes in AMP deaminase (AMPD) activity influence heart function and progression of heart disease, but the underlying mechanism is unknown. We evaluated the effect of purine riboside (Purr) on the activity of AMPD in perfused rat hearts and in isolated rat cardiomyocytes. Brief perfusion of the pre-ischemic heart with 200 μ M Purr resulted in activation of AMPD, more pronounced degradation of the adenine nucleotides, and reduced recovery of the adenine nucleotide pool during reperfusion. Brief incubation of rat cardiomyocytes with 200 μ M Purr also activated AMPD, while prolonged exposure resulted in enzyme inhibition. We conclude that Purr activates AMPD, whereas metabolites of this compound may inhibit the enzyme.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:1525-7770
1532-2335
DOI:10.1080/15257770802146551