Search Results - "Lang, Dietmar"

Aggregates in monoclonal antibody manufacturing processes by Vázquez-Rey, María, Lang, Dietmar A.

“…Monoclonal antibodies have proved to be a highly successful class of therapeutic products. Large‐scale manufacturing of pharmaceutical antibodies is a complex…”
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Degumming raw silk by a halotolerant metalloprotease isolated from metabolites of Vibrio sp. LA-05 by Zhang, Hong, Li, Hui, Liu, Haoran, Lang, Dietmar A., Xu, Hai, Zhu, Hu

“…The crude sample of a halotolerant metalloprotease produced by Vibrio sp. LA-05 was systematically investigated as a silk degumming agent. The experimental…”
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Purification, characterization and potential applications of a novel halotolerant metalloprotease from marine bacterium Vibrio sp. LA‐05 by Zhang, Hong, Li, Hui, Lang, Dietmar A, Xu, Hai, Zhu, Hu

“…BACKGROUND Many reported proteases showed low activity and stability under harsh operating conditions, e.g. high pH, salinity and surfactants, which hinders…”
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Development of a Clonal and High-Yield Mammalian Cell Line for the Manufacturing of a Hyperactive Human DNase I with Extended Plasma Half-Life Using PASylation ® Technology by Stamm, Serge M, Wagner, Roland, Lang, Dietmar A, Skerra, Arne, Gebauer, Michaela

“…Cumulative evidence from several pre-clinical studies suggests that restoration of plasma DNase activity in a thrombo-inflammatory state may improve clinical…”
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The application of a halotolerant metalloprotease from marine bacterium Vibrio sp. LA-05 in liquid detergent formulations by Zhang, Hong, Li, Hui, Liu, Haoran, Lang, Dietmar A., Xu, Hai, Zhu, Hu

“…The application of a halotolerant metalloprotease from marine bacterium Vibrio sp. LA-05 in liquid detergent formulations was investigated in detail. The…”
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Generic Chemometric Models for Metabolite Concentration Prediction Based on Raman Spectra by Yousefi-Darani, Abdolrahim, Paquet-Durand, Olivier, Von Wrochem, Almut, Classen, Jens, Tränkle, Jens, Mertens, Mario, Snelders, Jeroen, Chotteau, Veronique, Mäkinen, Meeri, Handl, Alina, Kadisch, Marvin, Lang, Dietmar, Dumas, Patrick, Hitzmann, Bernd

“…Chemometric models for on-line process monitoring have become well established in pharmaceutical bioprocesses. The main drawback is the required calibration…”
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Rapid high-throughput characterisation, classification and selection of recombinant mammalian cell line phenotypes using intact cell MALDI-ToF mass spectrometry fingerprinting and PLS-DA modelling by Povey, Jane F., O’Malley, Christopher J., Root, Tracy, Martin, Elaine B., Montague, Gary A., Feary, Marc, Trim, Carol, Lang, Dietmar A., Alldread, Richard, Racher, Andrew J., Smales, C. Mark

“…•Methodology for prediction of CHO cell productivity early in cell line development.•Development of a CHO cell MALDI-ToF fingerprint database.•Evaluation of…”
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Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks by Ihling, Nina, Munkler, Lara Pauline, Berg, Christoph, Reichenbächer, Britta, Wirth, Johannes, Lang, Dietmar, Wagner, Roland, Büchs, Jochen

“…Cultivations of mammalian cells are routinely conducted in shake flasks. In contrast to instrumented bioreactors, reliable options for non-invasive,…”
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Plasminogen activator inhibitor 1. Structure of the native serpin, comparison to its other conformers and implications for serpin inactivation by Nar, Herbert, Bauer, Margit, Stassen, Jean-Marie, Lang, Dietmar, Gils, Ann, Declerck, Paul J

“…The crystal structure of a constitutively active multiple site mutant of plasminogen activator inhibitor 1 (PAI-1) was determined and refined at a resolution…”
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The open conformation of a Pseudomonas lipase by Schrag, Joseph D, Li, Yunge, Cygler, Miroslaw, Lang, Dietmar, Burgdorf, Tanja, Hecht, Hans-Juergen, Schmid, Rolf, Schomburg, Dietmar, Rydel, Timothy J, Oliver, Joel D, Strickland, Larry C, Dunaway, C Michelle, Larson, Steven B, Day, John, McPherson, Alexander

“…Background: The interfacial activation of lipases results primarily from conformational changes in the enzymes which expose the active site and provide a…”
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CE-MS/MS and CE-timsTOF to separate and characterize intramolecular disulfide bridges of monoclonal antibody subunits and their application for the assessment of subunit reduction protocols by Schairer, Jasmin, Römer, Jennifer, Lang, Dietmar, Neusüß, Christian

“…Characterization at the subunit level enables detailed mass spectrometric characterization of posttranslational modifications (PTMs) of monoclonal antibodies…”
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Structural investigations of the regio- and enantioselectivity of lipases by Lang, Dietmar A, Dijkstra, Bauke W

“…Although lipases are widely applied for the stereospecific resolution of racemic mixtures of esters, the atomic details of the factors that are responsible for…”
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Non‐invasive and time‐resolved measurement of the respiration activity of Chinese hamster ovary cells enables prediction of key culture parameters in shake flasks by Ihling, Nina, Munkler, Lara Pauline, Paul, Richard, Berg, Christoph, Reichenbächer, Britta, Kadisch, Marvin, Lang, Dietmar, Büchs, Jochen

“…Background Shake flasks are frequently used for mammalian cell suspension cultures. For process development and routine culture monitoring, information on…”
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Introducing oxygen transfer rate measurements as a novel method for time-resolved cytotoxicity assessment in shake flasks by Ihling, Nina, Munkler, Lara Pauline, Paul, Richard, Lang, Dietmar, Büchs, Jochen

“…Background Determining the cytotoxicity of test substances is essential for the safety assessment of chemicals. To quantify the cytotoxicity, dose–response…”
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The crystal structure of a hyper-thermophilic carboxylesterase from the archaeon Archaeoglobus fulgidus 1 1Edited by R. Huber by De Simone, Giuseppina, Menchise, Valeria, Manco, Giuseppe, Mandrich, Luigi, Sorrentino, Nicola, Lang, Dietmar, Rossi, Mosè, Pedone, Carlo

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Structural basis of the chiral selectivity of Pseudomonas cepacia lipase by Lang, Dietmar A., Mannesse, Maurice L. M., De Haas, Gerard H., Verheij, Hubertus M., Dijkstra, Bauke W.

“…To investigate the enantioselectivity of Pseudomonas cepacia lipase, inhibition studies were performed with SC‐ and RC‐(RP…”
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Structural Evidence for Evolution of the β/α Barrel Scaffold by Gene Duplication and Fusion by Lang, Dietmar, Thoma, Ralf, Henn-Sax, Martina, Sterner, Reinhard, Wilmanns, Matthias

“…The atomic structures of two proteins in the histidine biosynthesis pathway consist of β/α barrels with a twofold repeat pattern. It is likely that these…”
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Crystal structure of pseudomonas aeruginosa lipase in the open conformation. The prototype for family I.1 of bacterial lipases by Nardini, M, Lang, D A, Liebeton, K, Jaeger, K E, Dijkstra, B W

“…The x-ray structure of the lipase from Pseudomonas aeruginosa PAO1 has been determined at 2.54 A resolution. It is the first structure of a member of homology…”
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Crystal Structure of Pseudomonas aeruginosa Lipase in the Open Conformation by Nardini, Marco, Lang, Dietmar A., Liebeton, Klaus, Jaeger, Karl-Erich, Dijkstra, Bauke W.

“…The x-ray structure of the lipase fromPseudomonas aeruginosa PAO1 has been determined at 2.54 Å resolution. It is the first structure of a member of homology…”
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Crystal Structure of a Bacterial Lipase fromChromobacterium viscosumATCC 6918 Refined at 1.6 Å Resolution by Lang, Dietmar, Hofmann, Birgit, Haalck, Lutz, Hecht, Hans-Jürgen, Spener, Friedrich, Schmid, Rolf D., Schomburg, Dietmar

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