CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis

CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis

Objective Peri-implantitis is characterized by peri-implant mucositis and alveolar bone resorption. This study investigated cholecystokinin (CCK) expression and the mechanism underlying its involvement in peri-implantitis. Methods mRNA sequencing was performed using the Gene Expression Omnibus datab...

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Bibliographic Details
Published in:Journal of international medical research Vol. 50; no. 12; p. 3000605221141312
Main Authors: Chou, LongHang, Chang, YaTing, Lan, KaiWen, Liu, Meng, Lu, YuKun, Li, XiaoLei, Li, PeiRu, Xu, Yue
Format: Journal Article
Language:English
Published: London, England SAGE Publications 01-12-2022
Sage Publications Ltd
SAGE Publishing
Subjects:
Cell Differentiation
Cells, Cultured
Cholecystokinin
Gene expression
Humans
NF-kappa B - genetics
NF-kappa B - metabolism
Osteogenesis - genetics
Prospective Clinical Research Report
Signal Transduction
NF-κB
TNFα
human bone marrow mesenchymal stem cells
Cholecystokinin
osteogenic differentiation
peri-implantitis
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Summary:Objective Peri-implantitis is characterized by peri-implant mucositis and alveolar bone resorption. This study investigated cholecystokinin (CCK) expression and the mechanism underlying its involvement in peri-implantitis. Methods mRNA sequencing was performed using the Gene Expression Omnibus database GSE106090. Human bone marrow mesenchymal stem cells (hBMSCs) were pretreated with various concentrations of CCK (0, 10, 30, or 100 nM) for 1 hour before induction in osteogenic differentiation medium for 2 weeks. Alkaline phosphatase (ALP) activity was determined, and the cells were stained with alizarin red. The expression levels of TNFα and the osteogenic markers ALP, RUNX2, and OCN were measured using quantitative real-time PCR. TNFα, phosphorylated P65, and total P65 levels were determined by western blot. Results Compared with healthy individuals, 262 and 215 genes were up- and down-regulated, respectively, in the periodontal tissues of patients with peri-implantitis. CCK expression was significantly upregulated in patients with peri-implantitis. CCK reduced ALP activity, osteogenic differentiation, and levels of the osteogenic markers ALP, RUNX2, and OCN. Moreover, CCK promoted levels of TNFα and phosphorylated P65, which is a marker of activation for the NF-κB inflammatory pathway. Conclusions CCK regulates osteogenic differentiation through the TNFα/NF-κB axis in peri-implantitis.
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These authors contributed equally to this work.
ISSN:0300-0605
1473-2300
DOI:10.1177/03000605221141312