'normalization' of germ-free guineapigs with host-specific caecal microflora

'normalization' of germ-free guineapigs with host-specific caecal microflora

Hysterectomy-derived germ-free guineapigs were given colonization-resistant caecal flora from mice (mCRF) or microflora obtained from the caecum of an antibiotic-decontaminated conventional guineapig (gpCRF) and compared with guineapigs raised conventionally with the sow. Body weight and the followi...

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Bibliographic Details
Published in:Laboratory animals (London) Vol. 23; no. 1; pp. 48 - 52
Main Authors: Boot, R, Koopman, J.P, Kruijt, B.C, Lammers, R.M, Kennis, H.M, Lankhorst, A, Welling, G.W, Hectors, M.P.C
Format: Journal Article
Language:English
Published: London, England SAGE Publications 1989
Subjects:
Animals
Bacterial Infections - immunology
Bacterial Infections - veterinary
Body Weight - veterinary
cecum
Cecum - microbiology
Enterobacteriaceae
Escherichia coli - growth & development
Fatty Acids - metabolism
Germ-Free Life
germfree state
guinea pigs
Guinea Pigs - microbiology
Mice
Organ Size - veterinary
resistance
GERM-FREE
COLONIZATION RESISTANCE
GUINEAPIGS
ENTEROBACTERIACEAE
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Summary:Hysterectomy-derived germ-free guineapigs were given colonization-resistant caecal flora from mice (mCRF) or microflora obtained from the caecum of an antibiotic-decontaminated conventional guineapig (gpCRF) and compared with guineapigs raised conventionally with the sow. Body weight and the following intestinal parameters were determined for the groups: colonization resistance (CR) to Escherichia coli, relative caecal weight (RCW), beta-aspartylglycine (faeces), volatile fatty acids (caecum) and bile acids (faeces). mCRF guineapigs showed values quite different from control animals for CR and RCW, indicating the unsuitability of mouse CRF for normalizing guineapigs. In gpCRF guineapigs CR and RCW values were comparable with controls, indicating the suitability of the guineapig flora for normalizing guineapigs. mCRF guineapigs housed with gpCRF guineapigs, showed an improvement in CR and RCW, yielding values found in control animals.
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content type line 23
ISSN:0023-6772
1758-1117
DOI:10.1258/002367789780886867