Substantial benefits of an inert biphenyl column for the analysis of steroids and their phase II metabolites in biological samples

Substantial benefits of an inert biphenyl column for the analysis of steroids and their phase II metabolites in biological samples

Steroids can be used as biomarkers in clinical metabolomics and other fields related to human toxicology. This chemical group is known for its complexity, considering its number of isobaric compounds and the wide variety of phases I and II metabolic pathways that parent compounds can undergo. For a...

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Bibliographic Details
Published in:Journal of separation science Vol. 47; no. 16; pp. e2400436 - n/a
Main Authors: Galmiche, Mathieu, Monat, Marie‐Anaïs, Lopez, Diego A., Lamboley, Cyrille, Connolly, Paul, Girel, Sergey, Guillarme, Davy, Meister, Isabel, Rudaz, Serge
Format: Journal Article
Language:English
Published: Germany Wiley Subscription Services, Inc 01-08-2024
Subjects:
Adsorption
Biocompatibility
Biological properties
Biomarkers
Biphenyl Compounds
Chemical compounds
Chromatography, High Pressure Liquid
glucuronides
Hardware
Humans
Liquid chromatography
Mass spectrometry
Metabolites
non‐specific adsorption
Performance degradation
Plasma
Reagents
Separation
Solvent extraction processes
Steroids
Steroids - analysis
Steroids - blood
Steroids - metabolism
sulfates
Toxicology
liquid chromatography
steroids
non‐specific adsorption
sulfates
glucuronides
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Summary:Steroids can be used as biomarkers in clinical metabolomics and other fields related to human toxicology. This chemical group is known for its complexity, considering its number of isobaric compounds and the wide variety of phases I and II metabolic pathways that parent compounds can undergo. For a successful analysis of steroids in biological samples, liquid chromatography separation must be finely tuned. It is especially challenging for glucuronidated and sulfated steroids derivatives that bear polar heads and can be affected by non‐specific adsorption. The benefits of a biphenyl stationary phase chemistry for the selectivity of the separation of steroids and their phase II metabolites and the extent to which nonspecific adsorption phenomena could degrade chromatographic performance were investigated. Replacing a conventional hardware by a passivated hardware allowed to considerably reduce peaks width and asymmetry of sulfated species. The addition of weak ion pairing agents in the mobile phase could also help to reduce non‐specific adsorption but are detrimental to mass spectrometry detection. As confirmed by the successful detection of 52 steroids in plasma, the use of a biphenyl stationary phase complemented by a passivated column hardware is of great help for a successful biomedical analysis of steroids and their phase II metabolites.
Bibliography:This paper is included in the Special Collection ‘Stationary Phase and Column Technologies’ edited by Michael Laemmerhofer.
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ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.202400436