Search Results - "Lam, Stéphanie"

Directed evolution of APEX2 for electron microscopy and proximity labeling by Lam, Stephanie S, Martell, Jeffrey D, Kamer, Kimberli J, Deerinck, Thomas J, Ellisman, Mark H, Mootha, Vamsi K, Ting, Alice Y

“…A genetically encoded peroxidase with improved sensitivity, APEX2, is reported for electron microscopy and proximity labeling at low expression levels. APEX is…”
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Spatially resolved proteomic mapping in living cells with the engineered peroxidase APEX2 by Hung, Victoria, Udeshi, Namrata D, Lam, Stephanie S, Loh, Ken H, Cox, Kurt J, Pedram, Kayvon, Carr, Steven A, Ting, Alice Y

“…In this protocol, Hung et al . describe a method for performing cell compartment–specific proteomics for regions of interest using the engineered ascorbate…”
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Proteomic mapping of cytosol-facing outer mitochondrial and ER membranes in living human cells by proximity biotinylation by Hung, Victoria, Lam, Stephanie S, Udeshi, Namrata D, Svinkina, Tanya, Guzman, Gaelen, Mootha, Vamsi K, Carr, Steven A, Ting, Alice Y

“…The cytosol-facing membranes of cellular organelles contain proteins that enable signal transduction, regulation of morphology and trafficking, protein import…”
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Electron microscopy using the genetically encoded APEX2 tag in cultured mammalian cells by Martell, Jeffrey D, Deerinck, Thomas J, Lam, Stephanie S, Ellisman, Mark H, Ting, Alice Y

“…This protocol describes procedures for using the genetic tag APEX2 to generate contrast for electron microscopy in cultured cells. Electron microscopy (EM) is…”
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MitoCarta3.0: an updated mitochondrial proteome now with sub-organelle localization and pathway annotations by Rath, Sneha, Sharma, Rohit, Gupta, Rahul, Ast, Tslil, Chan, Connie, Durham, Timothy J, Goodman, Russell P, Grabarek, Zenon, Haas, Mary E, Hung, Wendy H W, Joshi, Pallavi R, Jourdain, Alexis A, Kim, Sharon H, Kotrys, Anna V, Lam, Stephanie S, McCoy, Jason G, Meisel, Joshua D, Miranda, Maria, Panda, Apekshya, Patgiri, Anupam, Rogers, Robert, Sadre, Shayan, Shah, Hardik, Skinner, Owen S, To, Tsz-Leung, Walker, Melissa A, Wang, Hong, Ward, Patrick S, Wengrod, Jordan, Yuan, Chen-Ching, Calvo, Sarah E, Mootha, Vamsi K

“…Abstract The mammalian mitochondrial proteome is under dual genomic control, with 99% of proteins encoded by the nuclear genome and 13 originating from the…”
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Crystal growth and scintillation performance of Cs2HfCl6 and Cs2HfCl4Br2 by Lam, Stephanie, Guguschev, Christo, Burger, Arnold, Hackett, Maria, Motakef, Shariar

“…•Single crystals of Cs2HfCl6 and Cs2HfCl4Br2 were grown by the Bridgman technique.•Cs2HfCl6 has a light yield of 30,000 ph/MeV, and an energy resolution of…”
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Systematic Selection of Reference Genes for the Normalization of Circulating RNA Transcripts in Pregnant Women Based on RNA-Seq Data by Chim, Stephen S C, Wong, Karen K W, Chung, Claire Y L, Lam, Stephanie K W, Kwok, Jamie S L, Lai, Chit-Ying, Cheng, Yvonne K Y, Hui, Annie S Y, Meng, Meng, Chan, Oi-Ka, Tsui, Stephen K W, Lee, Keun-Young, Chan, Ting-Fung, Leung, Tak-Yeung

“…RNA transcripts circulating in peripheral blood represent an important source of non-invasive biomarkers. To accurately quantify the levels of circulating…”
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Isolation of >1 nm Diameter Single-Wall Carbon Nanotube Species Using Aqueous Two-Phase Extraction by Fagan, Jeffrey A, Hároz, Erik H, Ihly, Rachelle, Gui, Hui, Blackburn, Jeffrey L, Simpson, Jeffrey R, Lam, Stephanie, Hight Walker, Angela R, Doorn, Stephen K, Zheng, Ming

“…In this contribution we demonstrate the effective separation of single-wall carbon nanotube (SWCNT) species with diameters larger than 1 nm through multistage…”
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High-Throughput Combinatorial Database of Electronic Band Structures for Inorganic Scintillator Materials by Setyawan, Wahyu, Gaume, Romain M, Lam, Stephanie, Feigelson, Robert S, Curtarolo, Stefano

“…For the purpose of creating a database of electronic structures of all the known inorganic compounds, we have developed a computational framework based on…”
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People With Acute Low Back Pain Have Concerns That May Not Be Addressed by Guideline-Recommended Advice: A Mixed-Methods Study by Lam, Stephanie A, Zadro, Joshua R, Maher, Christopher G, San Juan, Charlene, Wang, Haiyi, Ferreira, Giovanni E

“…To investigate what concerns people with acute low back pain (LBP) and explore whether demographic and clinical factors were associated with having concerns…”
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Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation by Liu, Daniel S, Loh, Ken H, Lam, Stephanie S, White, Katharine A, Ting, Alice Y

“…Neurexin and neuroligin are transmembrane adhesion proteins that play an important role in organizing the neuronal synaptic cleft. Our lab previously reported…”
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A High-Performance CLYC(Ce)-PVT Composite for Neutron and Gamma Detection by Lam, Stephanie, Fiala, John, Hackett, Maria, Motakef, Shariar

“…In this paper, we fabricated and characterized a 50 mm diameter <inline-formula> <tex-math notation="LaTeX">\times30 </tex-math></inline-formula> mm composite…”
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Understanding phase equilibria and segregation in Bridgman growth of Cs2LiYCl6 scintillator by Ruta, Francesco L., Swider, Stacy, Lam, Stephanie, Feigelson, Robert S.

“…Cs2LiYCl6 (CLYC) is a commercial scintillator material having good energy resolution and dual gamma/neutron detection capabilities. CLYC crystals currently…”
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AutoComet: A fully automated algorithm to quickly and accurately analyze comet assays by Barbé, Lise, Lam, Stephanie, Holub, Austin, Faghihmonzavi, Zohreh, Deng, Minnie, Iyer, Rajshri, Finkbeiner, Steven

“…DNA damage is a common cellular feature seen in cancer and neurodegenerative disease, but fast and accurate methods for quantifying DNA damage are lacking…”
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Imaging flow cytometry shows monosomy 17 in circulating plasma cells in myeloma by Lam, Stephanie J, Mincherton, Thomas I, Hui, Henry Y L, Sidiqi, M Hasib, Fuller, Kathryn A, Erber, Wendy N

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Evaluation of RNA quality and functional transcriptome of beef longissimus thoracis over time post-mortem by Lam, Stephanie, Kommadath, Arun, López-Campos, Óscar, Prieto, Nuria, Aalhus, Jennifer, Juárez, Manuel, Dugan, Michael E. R, Vahmani, Payam

“…Evaluating RNA quality and transcriptomic profile of beef muscle over time post-mortem may provide insight into RNA degradation and underlying biological and…”
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From biobank and data silos into a data commons: convergence to support translational medicine by Asiimwe, Rebecca, Lam, Stephanie, Leung, Samuel, Wang, Shanzhao, Wan, Rachel, Tinker, Anna, McAlpine, Jessica N, Woo, Michelle M. M, Huntsman, David G, Talhouk, Aline

“…Abstract Background To drive translational medicine, modern day biobanks need to integrate with other sources of data (clinical, genomics) to support novel…”
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Stability and Viscoelasticity of Magneto-Pickering Foams by Blanco, Elena, Lam, Stephanie, Smoukov, Stoyan K, Velikov, Krassimir P, Khan, Saad A, Velev, Orlin D

“…We have developed a new class of bistable Pickering foams, which can remain intact for weeks at room temperature but can be destroyed rapidly and on-demand…”
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Imaging flow cytometric detection of del(17p) in bone marrow and circulating plasma cells in multiple myeloma by Mincherton, Thomas I., Lam, Stephanie J., Clarke, Sarah E., Hui, Henry Y. L., Malherbe, Jacques A. J., Chuah, Hun S., Sidiqi, M. Hasib, Fuller, Kathy A., Erber, Wendy N.

“…Background Detection of del(17p) in myeloma is generally performed by fluorescence in situ hybridization (FISH) on a slide with analysis of up to 200 nuclei…”
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Students as teachers by Silbert, Benjamin I, Lam, Stephanie JP, Henderson, Robert D, Lake, Fiona R

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