Search Results - "Lakshmipathy, U"

Identification and quantification of immune cell types using PCR-based PureQuant methylation assay by Lakshmipathy, U, Pradhan, S, Dargitz, C, Guzman, J

“…Background & AimDevelopment and manufacturing of cell based immunotherapies rely on methods that can accurately identify and quantify cell purity. Current…”
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Qualification of pluripotent stem cells and its derivatives using comprehensive molecular characterization tools by Lakshmipathy, U, Pradhan, S, Zarrabi, A, Dargitz, C

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A Sendai virus reprogramming system designed for clinical and translational research by MacArthur, C.C, Sridharan, M, Coggan, A, Lakshmipathy, U

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Establishing iPSC cell banks derived using reagents and workflows optimized for cell therapy manufacturing by MacArthur, C.C, Pradhan, S, Switalski, S, Landon, M, Lakshmipathy, U

“…Background & AimInduced pluripotent stem cell (iPSC) research is rapidly moving towards translational and clinical applications.These applications require…”
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Double strand break rejoining by mammalian mitochondrial extracts by Lakshmipathy, Uma, Campbell, Colin

“…DNA end-joining was measured by incubating linearized plasmid DNA with mitochondrial protein extracts. A spectrum of end-joined molecules ranging from…”
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Mitochondrial DNA ligase III function is independent of Xrcc1 by Lakshmipathy, Uma, Campbell, Colin

“…Hamster EM9 cells, which lack Xrcc1 protein, have reduced levels of DNA ligase III and are defective in nuclear base excision repair. The Xrcc1 protein…”
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Antisense-mediated decrease in DNA ligase III expression results in reduced mitochondrial DNA integrity by Lakshmipathy, U, Campbell, C

“…The human DNA ligase III gene encodes both nuclear and mitochondrial proteins. Abundant evidence supports the conclusion that the nuclear DNA ligase III…”
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QUALIFIABLE POTENCY ASSAY FOR CAR-T CELLS USING TWO SCALABLE PLATFORMS by Kandell, J., Sylakowski, K., Lakshmipathy, U.

“…Assessment of Chimeric Antigen Receptor (CAR) T-Cell function can be achieved using direct methods via cytotoxicity assay or via indirect surrogate method by…”
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1047 - Immunotherapy: ESTABLISHMENT OF LENTIVIRAL INFECTIOUS TITER AND VECTOR COPY NUMBER ASSAYS FOR TRANSLATIONAL APPLICATIONS by Kandell, J., Sylakowski, K., Lakshmipathy, U.

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Immunotherapy: ESTABLISHMENT OF LENTIVIRAL INFECTIOUS TITER AND VECTOR COPY NUMBER ASSAYS FOR TRANSLATIONAL APPLICATIONS by Kandell, J., Sylakowski, K., Lakshmipathy, U.

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Mammalian mitochondrial extracts possess DNA end-binding activity by Coffey, Gregory, Lakshmipathy, Uma, Campbell, Colin

“…Mammalian mitochondrial protein extracts possess DNA end-binding (DEB) activity. Protein binding to a 394 bp double-stranded DNA molecule was measured using an…”
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SURROGATE CELL LINE FOR DETERMINATION OF LENTIVIRUS INFECTIOUS TITER INTENDED FOR USE IN T CELL MODIFICATION by Beecher, B., Kandell, J., Sylakowski, K., MacArthur, C.C., Lakshmipathy, U.

“…Chimeric Antigen Receptor (CAR) T cells are leading modern cancer immunotherapies and next generational methods are focused on reducing the cost by optimizing…”
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EXAMINATION OF ALLOGENEIC DONOR SOURCES FOR THE DEVELOPMENT OF IMMUNOTHERAPIES by Villegas, V., Verbarendse, M., Sylakowski, K., MacArthur, C.C., Lakshmipathy, U.

“…Starting material is an important consideration when developing and manufacturing immunotherapies such as dendritic cell (DC) vaccines. Historically, DC…”
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A sensitive epigenetic immune cell counting assay for identity and purity testing of cellular therapies by Lakshmipathy, U., Guzman, J., Landon, M.

“…Manufacturing cell therapy products often rely on laborious cell isolation techniques that result in contaminating byproducts. In order to ensure product…”
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Process Development and Manufacturing: PRACTICAL APPROACHES TO ACCURATE MEASUREMENT OF CELL HEALTH DURING DIFFERENT STAGES OF CELL PROCESSING by Richards, C., Kandell, J., Snyder, R., Lakshmipathy, U.

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Immunotherapy: EXAMINATION OF ORTHOGONAL METHODS FOR THE DETERMINATION OF VECTOR COPY NUMBER by Kandell, J., Milian, S., Lakshmipathy, U., Snyder, R.

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A universal QPCR standard for determination of identity, safety and potency of lenti virus by Kandell, J., C. MacArthur, C., Sharp, J., Lakshmipathy, U.

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Universal cytotoxicity assay for potency testing of t cell therapies by Lakshmipathy, U., MacArthur, C., Revankar, C., Coggan, A.

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Process Development and Manufacturing: EFFECT OF DONOR AND METHOD VARIABILITY ON BIOMANUFACTURING PROCESS ESTABLISHMENT by Verbarendse, M., Sylakowski, K., MacArthur, C.C., Clarke, D., Maves, Y. Kato, Lakshmipathy, U.

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889 - Process Development and Manufacturing: EFFECT OF DONOR AND METHOD VARIABILITY ON BIOMANUFACTURING PROCESS ESTABLISHMENT by Verbarendse, M., Sylakowski, K., MacArthur, C.C., Clarke, D., Maves, Y. Kato, Lakshmipathy, U.

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