Search Results - "Lajoie, Marc"

Biocontainment of genetically modified organisms by synthetic protein design by Mandell, Daniel J., Lajoie, Marc J., Mee, Michael T., Takeuchi, Ryo, Kuznetsov, Gleb, Norville, Julie E., Gregg, Christopher J., Stoddard, Barry L., Church, George M.

“…Genetically modified organisms (GMOs) are increasingly deployed at large scales and in open environments. Genetic biocontainment strategies are needed to…”
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Rewriting the Genetic Code by Mukai, Takahito, Lajoie, Marc J, Englert, Markus, Söll, Dieter

“…The genetic code-the language used by cells to translate their genomes into proteins that perform many cellular functions-is highly conserved throughout…”
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Codon usage of highly expressed genes affects proteome-wide translation efficiency by Frumkin, Idan, Lajoie, Marc J., Gregg, Christopher J., Hornung, Gil, Church, George M., Pilpel, Yitzhak

“…Although the genetic code is redundant, synonymous codons for the same amino acid are not used with equal frequencies in genomes, a phenomenon termed “codon…”
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De novo design of modular and tunable protein biosensors by Quijano-Rubio, Alfredo, Yeh, Hsien-Wei, Park, Jooyoung, Lee, Hansol, Langan, Robert A., Boyken, Scott E., Lajoie, Marc J., Cao, Longxing, Chow, Cameron M., Miranda, Marcos C., Wi, Jimin, Hong, Hyo Jeong, Stewart, Lance, Oh, Byung-Ha, Baker, David

“…Naturally occurring protein switches have been repurposed for the development of biosensors and reporters for cellular and clinical applications 1 . However,…”
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Genomically Recoded Organisms Expand Biological Functions by Lajoie, Marc J., Rovner, Alexis J., Goodman, Daniel B., Aerni, Hans-Rudolf, Haimovich, Adrian D., Kuznetsov, Gleb, Mercer, Jaron A., Wang, Harris H., Carr, Peter A., Mosberg, Joshua A., Rohland, Nadin, Schultz, Peter G., Jacobson, Joseph M., Rinehart, Jesse, Church, George M., Isaacs, Farren J.

“…We describe the construction and characterization of a genomically recoded organism (GRO). We replaced all known UAG stop codons in Escherichia coli MG1655…”
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De novo design of bioactive protein switches by Langan, Robert A., Boyken, Scott E., Ng, Andrew H., Samson, Jennifer A., Dods, Galen, Westbrook, Alexandra M., Nguyen, Taylor H., Lajoie, Marc J., Chen, Zibo, Berger, Stephanie, Mulligan, Vikram Khipple, Dueber, John E., Novak, Walter R. P., El-Samad, Hana, Baker, David

“…Allosteric regulation of protein function is widespread in biology, but is challenging for de novo protein design as it requires the explicit design of…”
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Designed protein logic to target cells with precise combinations of surface antigens by Lajoie, Marc J, Boyken, Scott E, Salter, Alexander I, Bruffey, Jilliane, Rajan, Anusha, Langan, Robert A, Olshefsky, Audrey, Muhunthan, Vishaka, Bick, Matthew J, Gewe, Mesfin, Quijano-Rubio, Alfredo, Johnson, JayLee, Lenz, Garreck, Nguyen, Alisha, Pun, Suzie, Correnti, Colin E, Riddell, Stanley R, Baker, David

“…Precise cell targeting is challenging because most mammalian cell types lack a single surface marker that distinguishes them from other cells. A solution would…”
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Evolution of a designed protein assembly encapsulating its own RNA genome by Butterfield, Gabriel L., Lajoie, Marc J., Gustafson, Heather H., Sellers, Drew L., Nattermann, Una, Ellis, Daniel, Bale, Jacob B., Ke, Sharon, Lenz, Garreck H., Yehdego, Angelica, Ravichandran, Rashmi, Pun, Suzie H., King, Neil P., Baker, David

“…Computationally designed icosahedral protein-based assemblies can protect their genetic material and evolve in biochemical environments, suggesting a route to…”
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Design, synthesis, and testing toward a 57-codon genome by Ostrov, Nili, Landon, Matthieu, Guell, Marc, Kuznetsov, Gleb, Teramoto, Jun, Cervantes, Natalie, Zhou, Minerva, Singh, Kerry, Napolitano, Michael G., Moosburner, Mark, Shrock, Ellen, Pruitt, Benjamin W., Conway, Nicholas, Goodman, Daniel B., Gardner, Cameron L., Tyree, Gary, Gonzales, Alexandra, Wanner, Barry L., Norville, Julie E., Lajoie, Marc J., Church, George M.

“…Recoding–the repurposing of genetic codons–is a powerful strategy for enhancing genomes with functions not commonly found in nature. Here, we report…”
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An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes by Italia, James S, Addy, Partha Sarathi, Wrobel, Chester J J, Crawford, Lisa A, Lajoie, Marc J, Zheng, Yunan, Chatterjee, Abhishek

“…In Escherichia coli , replacement of the endogenous tryptophanyl–tRNA synthetase–tRNA pair with its counterpart from Saccharomyces cerevisiae liberates the…”
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Improved bacterial recombineering by parallelized protein discovery by Wannier, Timothy M., Nyerges, Akos, Kuchwara, Helene M., Czikkely, Márton, Balogh, Dávid, Filsinger, Gabriel T., Borders, Nathaniel C., Gregg, Christopher J., Lajoie, Marc J., Rios, Xavier, Pál, Csaba, Church, George M.

“…Exploiting bacteriophage-derived homologous recombination processes has enabled precise, multiplex editing of microbial genomes and the construction of…”
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Precise Manipulation of Chromosomes in Vivo Enables Genome-Wide Codon Replacement by Isaacs, Farren J., Carr, Peter A., Wang, Harris H., Lajoie, Marc J., Sterling, Bram, Kraal, Laurens, Tolonen, Andrew C., Gianoulis, Tara A., Goodman, Daniel B., Reppas, Nikos B., Emig, Christopher J., Bang, Duhee, Hwang, Samuel J., Jewett, Michael C., Jacobson, Joseph M., Church, George M.

“…We present genome engineering technologies that are capable of fundamentally reengineering genomes from the nucleotide to the megabase scale. We used multiplex…”
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A split, conditionally active mimetic of IL-2 reduces the toxicity of systemic cytokine therapy by Quijano-Rubio, Alfredo, Bhuiyan, Aladdin M., Yang, Huilin, Leung, Isabel, Bello, Elisa, Ali, Lestat R., Zhangxu, Kevin, Perkins, Jilliane, Chun, Jung-Ho, Wang, Wentao, Lajoie, Marc J., Ravichandran, Rashmi, Kuo, Yun-Huai, Dougan, Stephanie K., Riddell, Stanley R., Spangler, Jamie B., Dougan, Michael, Silva, Daniel-Adriano, Baker, David

“…The therapeutic potential of recombinant cytokines has been limited by the severe side effects of systemic administration. We describe a strategy to reduce the…”
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Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases by Mosberg, Joshua A, Gregg, Christopher J, Lajoie, Marc J, Wang, Harris H, Church, George M

“…Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of…”
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Building smart CAR T cell therapies: The path to overcome current challenges by Cai, Qi, Warren, Sarah, Pietrobon, Violena, Maeurer, Markus, Qi, Lei S., Lu, Timothy K., Lajoie, Marc J., Barrett, David, Stroncek, David F., Marincola, Francesco M.

“…Successful implementation of adoptive cell therapy (ACT) of cancer requires comprehensively addressing biological and practical challenges. This approach has…”
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The Genome Project–Write by Boeke, Jef D., Church, George, Hessel, Andrew, Kelley, Nancy J., Arkin, Adam, Cai, Yizhi, Carlson, Rob, Chakravarti, Aravinda, Cornish, Virginia W., Holt, Liam, Isaacs, Farren J., Kuiken, Todd, Lajoie, Marc, Lessor, Tracy, Lunshof, Jeantine, Maurano, Matthew T., Mitchell, Leslie A., Rine, Jasper, Rosser, Susan, Sanjana, Neville E., Silver, Pamela A., Valle, David, Wang, Harris, Way, Jeffrey C., Yang, Luhan

“…We need technology and an ethical framework for genome-scale engineering The Human Genome Project (“HGP-read”), nominally completed in 2004, aimed to sequence…”
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Characterizing the portability of phage-encoded homologous recombination proteins by Filsinger, Gabriel T., Wannier, Timothy M., Pedersen, Felix B., Lutz, Isaac D., Zhang, Julie, Stork, Devon A., Debnath, Anik, Gozzi, Kevin, Kuchwara, Helene, Volf, Verena, Wang, Stan, Rios, Xavier, Gregg, Christopher J., Lajoie, Marc J., Shipman, Seth L., Aach, John, Laub, Michael T., Church, George M.

“…Efficient genome editing methods are essential for biotechnology and fundamental research. Homologous recombination (HR) is the most versatile method of genome…”
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Multiplex pairwise assembly of array-derived DNA oligonucleotides by Klein, Jason C, Lajoie, Marc J, Schwartz, Jerrod J, Strauch, Eva-Maria, Nelson, Jorgen, Baker, David, Shendure, Jay

“…While the cost of DNA sequencing has dropped by five orders of magnitude in the past decade, DNA synthesis remains expensive for many applications. Although…”
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In vivo selection of synthetic nucleocapsids for tissue targeting by Olshefsky, Audrey, Benasutti, Halli, Sylvestre, Meilyn, Butterfield, Gabriel L, Rocklin, Gabriel J, Richardson, Christian, Hicks, Derrick R, Lajoie, Marc J, Song, Kefan, Leaf, Elizabeth, Treichel, Catherine, Decarreau, Justin, Ke, Sharon, Kher, Gargi, Carter, Lauren, Chamberlain, Jeffrey S, Baker, David, King, Neil P, Pun, Suzie H

“…Controlling the biodistribution of protein- and nanoparticle-based therapeutic formulations remains challenging. In vivo library selection is an effective…”
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Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection by Carr, Peter A, Wang, Harris H, Sterling, Bram, Isaacs, Farren J, Lajoie, Marc J, Xu, George, Church, George M, Jacobson, Joseph M

“…Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is…”
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