Immunological detection of spectrin during differentiation and in mature ciliated cells from quail oviduct

Immunological detection of spectrin during differentiation and in mature ciliated cells from quail oviduct

A protein that was immunologically related to the erythrocyte and brain alpha-240-subunit and to the brain beta-235-subunit of spectrin was characterized by immunoblotting and was detected by immunofluorescence in the apical part of ciliated cells from quail oviduct. After immunogold-labeling electr...

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Bibliographic Details
Published in:Journal of cell science Vol. 93; no. 4; pp. 683 - 690
Main Authors: Chailley, B, Frappier, T, Regnouf, F, Laine, M.C
Format: Journal Article
Language:English
Published: Cambridge Company of Biologists 01-08-1989
Subjects:
Animals
binding proteins
Biological and medical sciences
Cell Differentiation
Cell differentiation, maturation, development, hematopoiesis
Cell Membrane - analysis
Cell physiology
Cilia - analysis
Coturnix
Coturnix coturnix
detection
electron microscopy
Female
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Immunoblotting
Immunohistochemistry
immunology
Microscopy, Electron
Microvilli - analysis
Molecular and cellular biology
oviducts
Oviducts - analysis
Oviducts - cytology
Oviducts - ultrastructure
spectrin
Spectrin - analysis
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Summary:A protein that was immunologically related to the erythrocyte and brain alpha-240-subunit and to the brain beta-235-subunit of spectrin was characterized by immunoblotting and was detected by immunofluorescence in the apical part of ciliated cells from quail oviduct. After immunogold-labeling electron immunocytochemistry, spectrin was detected mainly in a fibrillar meshwork located between the proximal parts of the basal bodies. It was also observed to be in contact with the basal foot of basal bodies, but was not found to be associated with the apical plasma membrane. Cilia and microvilli were unlabeled. In contrast, spectrin was detected in close contact with the lateral plasma membrane of mature ciliated cells as well as in stem epithelial cells in unstimulated oviduct. During ciliogenesis induced by estrogen, spectrin gradually appeared at the apex of the cells as the apical cytoskeleton differentiated.
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ISSN:0021-9533
1477-9137
DOI:10.1242/jcs.93.4.683