Search Results - "Lahr, Walker"

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays by Kurata, Morito, Wolf, Natalie K, Lahr, Walker S, Weg, Madison T, Kluesner, Mitchell G, Lee, Samantha, Hui, Kai, Shiraiwa, Masano, Webber, Beau R, Moriarity, Branden S

“…The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immunity in bacteria. This system has been adopted for numerous…”
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Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors by Webber, Beau R., Lonetree, Cara-lin, Kluesner, Mitchell G., Johnson, Matthew J., Pomeroy, Emily J., Diers, Miechaleen D., Lahr, Walker S., Draper, Garrett M., Slipek, Nicholas J., Smeester, Branden A., Lovendahl, Klaus N., McElroy, Amber N., Gordon, Wendy R., Osborn, Mark J., Moriarity, Branden S.

“…The fusion of genome engineering and adoptive cellular therapy holds immense promise for the treatment of genetic disease and cancer. Multiplex genome…”
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A Genetically Engineered Primary Human Natural Killer Cell Platform for Cancer Immunotherapy by Pomeroy, Emily J., Hunzeker, John T., Kluesner, Mitchell G., Lahr, Walker S., Smeester, Branden A., Crosby, Margaret R., Lonetree, Cara-lin, Yamamoto, Kenta, Bendzick, Laura, Miller, Jeffrey S., Geller, Melissa A., Walcheck, Bruce, Felices, Martin, Webber, Beau R., Starr, Timothy K., Moriarity, Branden S.

“…Enhancing natural killer (NK) cell cytotoxicity by blocking inhibitory signaling could lead to improved NK-based cancer immunotherapy. Thus, we have developed…”
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Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease by Johnson, Matthew J., Laoharawee, Kanut, Lahr, Walker S., Webber, Beau R., Moriarity, Branden S.

“…B cells offer unique opportunities for gene therapy because of their ability to secrete large amounts of protein in the form of antibody and persist for the…”
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Engineering T cells to enhance 3D migration through structurally and mechanically complex tumor microenvironments by Tabdanov, Erdem D., Rodríguez-Merced, Nelson J., Cartagena-Rivera, Alexander X., Puram, Vikram V., Callaway, Mackenzie K., Ensminger, Ethan A., Pomeroy, Emily J., Yamamoto, Kenta, Lahr, Walker S., Webber, Beau R., Moriarity, Branden S., Zhovmer, Alexander S., Provenzano, Paolo P.

“…Defining the principles of T cell migration in structurally and mechanically complex tumor microenvironments is critical to understanding escape from antitumor…”
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CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells by Kluesner, Mitchell G., Lahr, Walker S., Lonetree, Cara-lin, Smeester, Branden A., Qiu, Xiaohong, Slipek, Nicholas J., Claudio Vázquez, Patricia N., Pitzen, Samuel P., Pomeroy, Emily J., Vignes, Madison J., Lee, Samantha C., Bingea, Samuel P., Andrew, Aneesha A., Webber, Beau R., Moriarity, Branden S.

“…CRISPR-Cas9 cytidine and adenosine base editors (CBEs and ABEs) can disrupt genes without introducing double-stranded breaks by inactivating splice sites…”
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A BAFF ligand-based CAR-T cell targeting three receptors and multiple B cell cancers by Wong, Derek P., Roy, Nand K., Zhang, Keman, Anukanth, Anusha, Asthana, Abhishek, Shirkey-Son, Nicole J., Dunmire, Samantha, Jones, Bryan J., Lahr, Walker S., Webber, Beau R., Moriarity, Branden S., Caimi, Paolo, Parameswaran, Reshmi

“…B cell-activating factor (BAFF) binds the three receptors BAFF-R, BCMA, and TACI, predominantly expressed on mature B cells. Almost all B cell cancers are…”
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CRISPR-Cas9 base editors and their current role in human therapeutics by Lahr, Walker S., Sipe, Christopher J., Skeate, Joseph G., Webber, Beau R., Moriarity, Branden S.

“…Consistent progress has been made to create more efficient and useful CRISPR-Cas9-based molecular toolsfor genomic modification. This review focuses on recent…”
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Germline T cell receptor exchange results in physiological T cell development and function by Rollins, Meagan R., Raynor, Jackson F., Miller, Ebony A., Butler, Jonah Z., Spartz, Ellen J., Lahr, Walker S., You, Yun, Burrack, Adam L., Moriarity, Branden S., Webber, Beau R., Stromnes, Ingunn M.

“…T cell receptor (TCR) transgenic mice represent an invaluable tool to study antigen-specific immune responses. In the pre-existing models, a monoclonal TCR is…”
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A Pan-RNase Inhibitor Enabling CRISPR-mRNA Platforms for Engineering of Primary Human Monocytes by Laoharawee, Kanut, Johnson, Matthew J., Lahr, Walker S., Sipe, Christopher J., Kleinboehl, Evan, Peterson, Joseph J., Lonetree, Cara-lin, Bell, Jason B., Slipek, Nicholas J., Crane, Andrew T., Webber, Beau R., Moriarity, Branden S.

“…Monocytes and their downstream effectors are critical components of the innate immune system. Monocytes are equipped with chemokine receptors, allowing them to…”
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Evolution of the clinical-stage hyperactive TcBuster transposase as a platform for robust non-viral production of adoptive cellular therapies by Skeate, Joseph G, Pomeroy, Emily J, Slipek, Nicholas J, Jones, Bryan J, Wick, Bryce J, Chang, Jae-Woong, Lahr, Walker S, Stelljes, Erin M, Patrinostro, Xiaobai, Barnes, Blake, Zarecki, Trevor, Krueger, Joshua B, Bridge, Jacob E, Robbins, Gabrielle M, McCormick, Madeline D, Leerar, John R, Wenzel, Kari T, Hornberger, Kathlyn M, Walker, Kirsti, Smedley, Dalton, Largaespada, David A, Otto, Neil, Webber, Beau R, Moriarity, Branden S

“…Cellular therapies for the treatment of human diseases, such as chimeric antigen receptor (CAR) T and natural killer (NK) cells have shown remarkable clinical…”
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Correction of Fanconi Anemia Mutations Using Digital Genome Engineering by Sipe, Christopher J, Kluesner, Mitchell G, Bingea, Samuel P, Lahr, Walker S, Andrew, Aneesha A, Wang, Minjing, DeFeo, Anthony P, Hinkel, Timothy L, Laoharawee, Kanut, Wagner, John E, MacMillan, Margaret L, Vercellotti, Gregory M, Tolar, Jakub, Osborn, Mark J, McIvor, R Scott, Webber, Beau R, Moriarity, Branden S

“…Fanconi anemia (FA) is a rare genetic disease in which genes essential for DNA repair are mutated. Both the interstrand crosslink (ICL) and double-strand break…”
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Development and testing of a versatile genome editing application reporter (V-GEAR) system by Kleinboehl, Evan W., Laoharawee, Kanut, Lahr, Walker S., Jensen, Jacob D., Peterson, Joseph J., Bell, Jason B., Webber, Beau R., Moriarity, Branden S.

“…CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability combined with deaminases or reverse transcriptases have revolutionized…”
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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9 by Laoharawee, Kanut, Johnson, Matthew J, Lahr, Walker S, Peterson, Joseph J, Webber, Beau R, Moriarity, Branden S

“…B cells are lymphocytes derived from hematopoietic stem cells and are a key component of the humoral arm of the adaptive immune system. They make attractive…”
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Author Correction: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors by Webber, Beau R., Lonetree, Cara-lin, Kluesner, Mitchell G., Johnson, Matthew J., Pomeroy, Emily J., Diers, Miechaleen D., Lahr, Walker S., Draper, Garrett M., Slipek, Nicholas J., Smeester, Branden A., Lovendahl, Klaus N., McElroy, Amber N., Gordon, Wendy R., Osborn, Mark J., Moriarity, Branden S.

“…An amendment to this paper has been published and can be accessed via a link at the top of the paper…”
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Internal checkpoint regulates T cell neoantigen reactivity and susceptibility to PD1 blockade by Palmer, Douglas C, Webber, Beau R, Patel, Yogin, Johnson, Matthew J, Kariya, Christine M, Lahr, Walker S, Parkhurst, Maria R, Gartner, Jared J, Prickett, Todd D, Lowery, Frank J, Kishton, Rigel J, Gurusamy, Devikala, Franco, Zulmarie, Vodnala, Suman K, Diers, Miechaleen D, Wolf, Natalie K, Slipek, Nicholas J, McKenna, David H, Sumstad, Darin, Viney, Lydia, Henley, Tom, Bürckstümmer, Tilmann, Baker, Oliver, Hu, Ying, Yan, Chunhua, Meerzaman, Daoud, Padhan, Kartik, Lo, Winnie, Malekzadeh, Parisa, Jia, Li, Deniger, Drew C, Patel, Shashank J, Robbins, Paul F, McIvor, R Scott, Choudhry, Modassir, Rosenberg, Steven A, Moriarity, Branden S, Restifo, Nicholas P

“…Adoptive transfer of tumor-infiltrating lymphocytes (TIL) fails to consistently elicit tumor rejection. Manipulation of intrinsic factors that inhibit T cell…”
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Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair by Webber, Beau R, Johnson, Matthew J, Skeate, Joseph G, Slipek, Nicholas J, Lahr, Walker S, DeFeo, Anthony P, Mills, Lauren J, Qiu, Xiaohong, Rathmann, Blaine, Diers, Miechaleen D, Wick, Bryce, Henley, Tom, Choudhry, Modassir, Starr, Timothy K, McIvor, R Scott, Moriarity, Branden S

“…The reliance on viral vectors for the production of genetically engineered immune cells for adoptive cellular therapies remains a translational bottleneck…”
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333 Targeting the apical intracellular checkpoint CISH unleashes T cell neoantigen reactivity and effector program by Palmer, Douglas, Webber, Beau, Patel, Yogin, Johnson, Matthew, Kariya, Christine, Lahr, Walker, Parkhurst, Maria, Gartner, Jared, Prickett, Todd, Lowery, Frank, Kishton, Rigel, Gurusamy, Devikala, Franco, Zulmarie, Vodnala, Suman, Diers, Miechaleen, Wolf, Natalie, Slipek, Nicholas, McKenna, David, Sumstad, Darin, Viney, Lydia, Henley, Tom, Bürckstümmer, Tilmann, Baker, Oliver, Hu, Ying, Yan, Chunhua, Meerzaman, Daoud, Padhan, Kartik, Lo, Winnie, Malekzadeh, Parisa, Jia, Li, Deniger, Drew, Patel, Shashank, Robbins, Paul, Scott McIvor, R, Choudhry, Modassir, Rosenberg, Steven, Moriarity, Branden, Restifo, Nicholas

“…BackgroundNeoantigen-specific T cells isolated from tumors have shown promise clinically but fail to consistently elicit durable tumor regression. Expression…”
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3145 – TRANSIENT EXPRESSION OF HEMATOPOIETIC TRANSCRIPTION FACTORS ENHANCES T CELL POTENTIAL OF HUMAN INDUCED PLURIPOTENT STEM CELL-DERIVED HEMATOPOIETIC PROGENITORS by Napiwocki, Brett, Lahr, Walker, Moriarity, Branden, von Dissen, Lage, Webber, Beau

“…T cells derived from human pluripotent stem cells (PSCs) hold tremendous therapeutic potential, yet differentiation to definitive hematopoietic progenitors…”
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Abstract A021: Chimeric antigen receptor armored natural killer cell immunotherapy for osteosarcoma by Robbins, Gabrielle, Yamomoto, Kenta, Lahr, Walker, Skeate, Joseph

“…Abstract Over the last decade, Chimeric Antigen Receptor based T cell (CAR-T) therapy has developed into an effective immunotherapy for several cancers,…”
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