Chlorine-based DUWL disinfectant leads to a different microbial composition of water derived biofilms compared to H2O2-based chemical disinfectants in vitro

Chlorine-based DUWL disinfectant leads to a different microbial composition of water derived biofilms compared to H2O2-based chemical disinfectants in vitro

Background Biofilm formation in dental unit waterlines (DUWL) may lead to health risks for dental staff and patients. Therefore, dental unit waterlines need to be disinfected, for instance by using chemical disinfectants. However, the application of chemical disinfectants may lead to the selection o...

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Published in:PeerJ (San Francisco, CA) Vol. 8; p. e9503
Main Authors: Zemouri, Charifa, Laheij, Alexa MGA, Catherine MC Volgenant, Brandt, Bernd W, Crielaard, Wim, Buijs, Mark J, Egija Zaura, de Soet, Johannes J
Format: Journal Article
Language:English
Published: San Diego PeerJ, Inc 15-07-2020
PeerJ Inc
Subjects:
Antimicrobial agents
Bacteria
Biofilm
Biofilms
Chlorine
Dental unit waterlines
Dentistry
Deoxyribonucleic acid
Disinfectants
Disinfection
Disinfection & disinfectants
DNA
Drinking water
Effluents
Flow velocity
Hydrogen peroxide
Infections
Load
Microbiology
Microbiome
Microorganisms
Patients
Principal components analysis
Public Health
rRNA 16S
Sequencing
Water quality
Netherlands
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Summary:Background Biofilm formation in dental unit waterlines (DUWL) may lead to health risks for dental staff and patients. Therefore, dental unit waterlines need to be disinfected, for instance by using chemical disinfectants. However, the application of chemical disinfectants may lead to the selection of specific microorganisms. Therefore, the aim of our study was to assess the microbial composition of water-derived biofilms, after a continuous exposure to maintenance doses of commercially available chemical disinfectants, in vitro. Methods The AAA-model was used to grow water derived biofilms. The biofilms were subjected to the maintenance dose of each disinfectant. To determine the microbial composition, the V4 hypervariable region of the 16S rRNA gene was sequenced. The sequences were clustered in operational taxonomic units (OTUs). Results The bacterial composition of biofilms in all treatment groups differed significantly (PERMANOVA F = 4.441, p = 0.001). Pairwise comparisons revealed Anoxyl treated biofilms were significantly different from all groups (p = 0.0001). In the Anoxyl-treated biofilms, the relative abundance of Comamonadaceae and Sphingopyxis was high compared to the Dentosept, Green and Clean and Oxygenal groups. Conclusion We concluded that exposure to low doses of the chlorine-based chemical disinfectant Anoxyl led to a substantially different composition of water derived biofilms compared to biofilms exposed to H2O2-based chemical disinfectants.
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ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.9503