Search Results - "Lafer, EM"

Structural Basis of Interdomain Communication in the Hsc70 Chaperone by Jiang, Jianwen, Prasad, Kondury, Lafer, Eileen M., Sousa, Rui

“…Hsp70 family proteins are highly conserved chaperones involved in protein folding, degradation, targeting and translocation, and protein complex remodeling…”
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Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity by Farsi, Zohreh, Gowrisankaran, Sindhuja, Krunic, Matija, Rammner, Burkhard, Woehler, Andrew, Lafer, Eileen M, Mim, Carsten, Jahn, Reinhard, Milosevic, Ira

“…Newly-formed synaptic vesicles (SVs) are rapidly acidified by vacuolar adenosine triphosphatases (vATPases), generating a proton electrochemical gradient that…”
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Clathrin–Protein Interactions by Lafer, Eileen M.

“…There is a complex network of protein–protein and protein–lipid interactions that underlie clathrin‐mediated vesicular traffic in all compartmentalized cells…”
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Mitogen-Activated Protein Kinase Regulates Dopamine Transporter Surface Expression and Dopamine Transport Capacity by Moron, Jose A, Zakharova, Irina, Ferrer, Jasmine V, Merrill, Gerald A, Hope, Bruce, Lafer, Eileen M, Lin, Zhi Cheng, Wang, Jia Bei, Javitch, Jonathan A, Galli, Aurelio, Shippenberg, Toni S

“…The dopamine transporter (DAT) regulates the clearance of dopamine (DA) released into the extracellular space and is an important site on which…”
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Surface plasmon resonance (SPR) confirms that MEPE binds to PHEX via the MEPE–ASARM motif: a model for impaired mineralization in X-linked rickets (HYP) by Rowe, Peter S.N., Garrett, Ian R., Schwarz, Patricia M., Carnes, David L., Lafer, Eileen M., Mundy, Gregory R., Gutierrez, Gloria E.

“…Matrix Extracellular Phospho-glycoprotEin (MEPE) and proteases are elevated and PHEX is defective in HYP. PHEX prevents proteolysis of MEPE and release of a…”
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Measuring the Elasticity of Clathrin-Coated Vesicles via Atomic Force Microscopy by Jin, Albert J., Prasad, Kondury, Smith, Paul D., Lafer, Eileen M., Nossal, Ralph

“…Using a new scheme based on atomic force microscopy (AFM), we investigate mechanical properties of clathrin-coated vesicles (CCVs). CCVs are multicomponent…”
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PI 3‐kinase regulation of dopamine uptake by Carvelli, Lucia, Morón, José A., Kahlig, Kristopher M., Ferrer, Jasmine V., Sen, Namita, Lechleiter, James D., Leeb‐Lundberg, L. M. Fredrik, Merrill, Gerald, Lafer, Eileen M., Ballou, Lisa M., Shippenberg, Toni S., Javitch, Jonathan A., Lin, Richard Z., Galli, Aurelio

“…The magnitude and duration of dopamine (DA) signaling is defined by the amount of vesicular release, DA receptor sensitivity, and the efficiency of DA…”
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Uncoating of Clathrin-Coated Vesicles in Presynaptic Terminals: Roles for Hsc70 and Auxilin by Morgan, Jennifer R., Prasad, Kondury, Jin, Suping, Augustine, George J., Lafer, Eileen M.

“…We have examined the roles of Hsc70 and auxilin in the uncoating of clathrin-coated vesicles (CCVs) during neuronal endocytosis. We identified two peptides…”
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A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis by Morgan, Jennifer R, Prasad, Kondury, Hao, Weihua, Augustine, George J, Lafer, Eileen M

“…Although clathrin assembly by adaptor proteins (APs) plays a major role in the recycling of synaptic vesicles, the molecular mechanism that allows APs to…”
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Eps15 Homology Domain-NPF Motif Interactions Regulate Clathrin Coat Assembly during Synaptic Vesicle Recycling by Morgan, Jennifer R., Prasad, Kondury, Jin, Suping, Augustine, George J., Lafer, Eileen M.

“…Although genetic and biochemical studies suggest a role for Eps15 homology domain containing proteins in clathrin-mediated endocytosis, the specific functions…”
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AP180 and AP-2 Interact Directly in a Complex That Cooperatively Assembles Clathrin by Hao, W, Luo, Z, Zheng, L, Prasad, K, Lafer, E M

“…Clathrin-coated vesicles are involved in protein and lipid trafficking between intracellular compartments in eukaryotic cells. AP-2 and AP180 are the resident…”
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Clathrin and synaptic vesicle endocytosis: studies at the squid giant synapse by Augustine, G J, Morgan, J R, Villalba-Galea, C A, Jin, S, Prasad, K, Lafer, E M

“…The role of clathrin-mediated endocytosis in SV (synaptic vesicle) recycling has been studied by combining molecular biology, physiology and electron…”
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A Role for the Clathrin Assembly Domain of AP180 in Synaptic Vesicle Endocytosis by Morgan, Jennifer R, Zhao, Xiaojun, Womack, Mary, Prasad, Kondury, Augustine, George J, Lafer, Eileen M

“…We have used the squid giant synapse to determine whether clathrin assembly by AP180 is important for synaptic vesicle endocytosis. The squid homolog of AP180…”
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Structure−Function Analysis of the Auxilin J-Domain Reveals an Extended Hsc70 Interaction Interface by Jiang, Jianwen, Taylor, Alexander B, Prasad, Kondury, Ishikawa-Brush, Yumiko, Hart, P. John, Lafer, Eileen M, Sousa, Rui

“…J-domains are widespread protein interaction modules involved in recruiting and stimulating the activity of Hsp70 family chaperones. We have determined the…”
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Inhibition of Clathrin Assembly by High Affinity Binding of Specific Inositol Polyphosphates to the Synapse-specific Clathrin Assembly Protein AP-3 (∗) by Ye, Weilan, Ali, Nawab, Bembenek, Michael E., Shears, Stephen B., Lafer, Eileen M.

“…Bacterially expressed synapse-specific clathrin assembly protein, AP-3 (F1-20/AP180/NP185/pp155), bound with high affinity both inositol hexakisphosphate…”
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Regulation of AP-3 Function by Inositides by Hao, Weihua, Tan, Zheng, Prasad, Kondury, Reddy, K. Kishta, Chen, Jian, Prestwich, Glenn D., Falck, John R., Shears, Stephen B., Lafer, Eileen M.

“…As part of the growing effort to understand the role inositol phosphates and inositol lipids play in the regulation of vesicle traffic within nerve terminals,…”
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Bacterially expressed F1-20/AP-3 assembles clathrin into cages with a narrow size distribution: implications for the regulation of quantal size during neurotransmission by Ye, W, Lafer, E M

“…F1-20/AP-3 is a synapse-specific phosphoprotein. In this study we characterize the ability of bacterially expressed F1-20/AP-3 to bind and assemble clathrin…”
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Synaptic vesicle endocytosis: the races, places, and molecular faces by Morgan, Jennifer R, Augustine, George J, Lafer, Eileen M

“…The classical experiments on synaptic vesicle recycling in the 1970s by Heuser and Reese, Ceccarelli, and their colleagues raised opposing theories regarding…”
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Crystallization of a functionally intact Hsc70 chaperone by Jiang, Jianwen, Lafer, Eileen M., Sousa, Rui

“…Hsp70s are essential chaperones with roles in a variety of cellular processes and representatives in all kingdoms of life. They are comprised of a…”
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Clathrin Binding and Assembly Activities of Expressed Domains of the Synapse-specific Clathrin Assembly Protein AP-3 by Ye, Weilan, Lafer, Eileen M.

“…We separately expressed the 58-kDa C-terminal, 42-kDa middle, 16-kDa C-terminal, and 33-kDa N-terminal regions of AP-3 (also called F1-20, AP180, NP185, and…”
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