Search Results - "Laere, André Van"
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Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications
Published in Journal of experimental botany (01-03-2009)“…Glycoside hydrolases (GH) have been shown to play unique roles in various biological processes like the biosynthesis of glycans, cell wall metabolism, plant…”
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Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne
Published in Planta (01-09-2014)“…MAIN CONCLUSION : The first 6-fructan exohydrolase (6-FEH) cDNA from Lolium perenne was cloned and characterized. Following defoliation, Lp6 - FEHa transcript…”
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Donor and acceptor substrate selectivity among plant glycoside hydrolase family 32 enzymes
Published in The FEBS journal (01-10-2009)“…Plant family 32 glycoside hydrolase enzymes include hydrolases (cell wall invertases, fructan exohydrolases, vacuolar invertases) and fructosyltransferases…”
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Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.)
Published in Journal of experimental botany (01-01-2006)“…Fructans, {szligbeta}2-1 and/or {szligbeta}2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important…”
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X‐ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1‐exohydrolase IIa of Cichorium intybus
Published in The Plant journal : for cell and molecular biology (01-02-2005)“…Summary Fructan 1‐exohydrolase, an enzyme involved in fructan degradation, belongs to the glycosyl hydrolase family 32. The structure of isoenzyme 1‐FEH IIa…”
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Molecular and functional characterization of a cDNA encoding fructan:fructan 6G-fructosyltransferase (6G-FFT)/fructan:fructan 1-fructosyltransferase (1-FFT) from perennial ryegrass (Lolium perenne L.)
Published in Journal of experimental botany (2006)“…Fructans are the main storage compound in Lolium perenne. To account for the prevailing neokestose-based fructan synthesis in this species, a cDNA library of…”
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Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization
Published in Journal of experimental botany (01-01-2007)“…Fructans, which are β-(2,1) and/or β-(2,6) linked polymers of fructose, are important storage carbohydrates in many plants. They are mobilized via fructan…”
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Fructan 1-Exohydrolases. β-(2,1)-Trimmers during Graminan Biosynthesis in Stems of Wheat? Purification, Characterization, Mass Mapping, and Cloning of Two Fructan 1-Exohydrolase Isoforms
Published in Plant physiology (Bethesda) (01-02-2003)“…Graminan-type fructans are temporarily stored in wheat (Triticum aestivum) stems. Two phases can be distinguished: a phase of fructan biosynthesis (green…”
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Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase
Published in The New phytologist (01-05-2008)“…The hydrolytic plant enzymes of family 32 of glycoside hydrolases (GH32), including acid cell wall type invertases (EC 3.2.1.26), fructan 1-exohydrolases…”
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Creating S-type characteristics in the F-type enzyme fructan:fructan 1-fructosyltransferase of Triticum aestivum L
Published in Journal of experimental botany (01-09-2009)“…Invertases cleave sucrose in glucose and fructose, using water as an acceptor. Fructosyltransferases catalyse the transfer of a fructosyl residue between…”
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Properties of fructan:fructan 1-fructosyltransferases from chicory and globe thistle, two asteracean plants storing greatly different types of inulin
Published in Plant physiology (Bethesda) (01-09-2003)“…Remarkably, within the Asteraceae, a species-specific fructan pattern can be observed. Some species such as artichoke (Cynara scolymus) and globe thistle…”
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Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes
Published in Journal of experimental botany (01-03-2006)“…Inulin-type fructans are the simplest and most studied fructans and have become increasingly popular as prebiotic health-improving compounds. A natural…”
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Purification, cloning and functional differences of a third fructan 1-exohydrolase (1-FEHw3) from wheat (Triticum aestivum)
Published in Physiologia plantarum (01-06-2008)“…A third fructan exohydrolase isoform (1-FEHw3) was purified from wheat stems by a combination of ammonium sulfate precipitation, ConA affinity and ion-exchange…”
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Defoliation Induces Fructan 1-Exohydrolase II in Witloof Chicory Roots. Cloning and Purification of Two Isoforms, Fructan 1-Exohydrolase IIa and Fructan 1-Exohydrolase IIb. Mass Fingerprint of the Fructan 1-Exohydrolase II Enzymes
Published in Plant physiology (Bethesda) (01-07-2001)“…The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described…”
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The role of fructan in flowering of Campanula rapunculoides
Published in Journal of experimental botany (01-07-2000)“…Inulin type fructan was detected in all vegetative organs of Campanula rapunculoides L. plants. All flower parts contained fructan at some developmental stage…”
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Unexpected Presence of Graminan- and Levan-Type Fructans in the Evergreen Frost-Hardy Eudicot Pachysandra terminalis (Buxaceae): Purification, Cloning, and Functional Analysis of a 6-SST/6-SFT Enzyme
Published in Plant physiology (Bethesda) (01-01-2011)“…About 15% of flowering plants accumulate fructans. Inulin-type fructans with β(2,1) fructosyl linkages typically accumulate in the core eudicot families (e.g…”
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Transforming wheat vacuolar invertase into a high affinity sucrose:sucrose 1-fructosyltransferase
Published in The New phytologist (01-12-2008)“…Vacuolar invertases (VIs) degrade sucrose to glucose and fructose. Additionally, the fructan plant wheat (Triticum aestivum) contains different…”
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Unraveling the Difference between Invertases and Fructan Exohydrolases: A Single Amino Acid (Asp-239) Substitution Transforms Arabidopsis Cell Wall Invertase1 into a Fructan 1-Exohydrolase
Published in Plant physiology (Bethesda) (01-11-2007)“…Plant cell wall invertases and fructan exohydrolases (FEHs) are very closely related enzymes at the molecular and structural level (family 32 of glycoside…”
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Cloning, Developmental, and Tissue-Specific Expression of Sucrose:Sucrose 1-Fructosyl Transferase from Taraxacum officinale. Fructan Localization in Roots
Published in Plant physiology (Bethesda) (01-05-2000)“…Sucrose:sucrose 1-fructosyl transferase (1-SST) is the key enzyme initiating fructan synthesis in Asteraceae. Using reverse transcriptase-PCR, we isolated the…”
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Crystal Structures of Arabidopsis thaliana Cell-Wall Invertase Mutants in Complex with Sucrose
Published in Journal of molecular biology (21-03-2008)“…In plants, cell-wall invertases fulfil important roles in carbohydrate partitioning, growth, development and crop yield. In this study, we report on different…”
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