Quinolone resistance and ornithine decarboxylation activity in lactose-negative Escherichia coli

Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and q...

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Bibliographic Details
Published in:	Brazilian journal of microbiology Vol. 46; no. 3; pp. 753 - 757
Main Authors:	Gomig, Franciane, Galvão, Carolina Weigert, Freitas, Denis Leandro de, Labas, Larissa, Etto, Rafael Mazer, Esmerino, Luiz Antonio, Lima, Marcelo Andrade de, Appel, Marcia Helena, Zanata, Silvio Marques, Steffens, Maria Berenice Reynaud, Nader, Helena Bonciani, Silveira, Rafael Bertoni da
Format:	Journal Article
Language:	English
Published:	Brazil Springer Nature B.V 01-07-2015 Sociedade Brasileira de Microbiologia
Subjects:	Anti-Bacterial Agents - therapeutic use Antibiotics Bacterial infections Biochemistry Brazil Decarboxylation - genetics Decarboxylation - physiology DNA Gyrase - genetics DNA Topoisomerase IV - genetics Drug resistance Drug Resistance, Multiple, Bacterial - genetics E coli Escherichia coli Infections - drug therapy Escherichia coli Infections - microbiology Fluoroquinolones - therapeutic use Humans Lactose - metabolism Medical Microbiology Microbial Sensitivity Tests MICROBIOLOGY Nalidixic Acid - therapeutic use Ornithine - metabolism Ornithine Decarboxylase - genetics Urinary Tract Infections - drug therapy Urinary Tract Infections - microbiology Uropathogenic Escherichia coli - drug effects Uropathogenic Escherichia coli - enzymology Uropathogenic Escherichia coli - genetics Uropathogenic Escherichia coli - isolation & purification Brazil gyrA parC uropathogenic ODC
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Summary:	Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic E. coli strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic E. coli strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region gyrA and parC mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative E. coli isolates.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Associate Editor: Nilton Erbet Lincopan Huenuman
ISSN:	1678-4405 1517-8382 1678-4405
DOI:	10.1590/S1517-838246320131291