Search Results - "La Mar, Gerd N"

The Orbital Ground State of the Azide−Substrate Complex of Human Heme Oxygenase Is an Indicator of Distal H-Bonding: Implications for the Enzyme Mechanism by Ogura, Hiroshi, Evans, John P, Peng, Dungeng, Satterlee, James D, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…The active site electronic structure of the azide complex of substrate-bound human heme oxygenase 1 (hHO) has been investigated by 1H NMR spectroscopy to shed…”
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Solution 1H NMR characterization of substrate-free C. diphtheriae heme oxygenase: Pertinence for determining magnetic axes in paramagnetic substrate complexes by Du, Zhenming, Unno, Masaki, Matsui, Toshitaka, Ikeda-Saito, Masao, La Mar, Gerd N.

“…Proton 2D NMR was used to confirm in solution a highly conserved portion of the molecular structure upon substrate loss for the heme oxygenase from the…”
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Solution NMR Characterization of an Unusual Distal H-bond Network in the Active Site of the Cyanide-inhibited, Human Heme Oxygenase Complex of the Symmetric Substrate, 2,4-Dimethyldeuterohemin by Li, Yiming, Syvitski, Ray T, Auclair, Karine, Wilks, Angela, Ortiz De Montellano, Paul R, La Mar, Gerd N

“…The presence of variable static hemin orientational disorder about the α-γ-meso axis in the substrate complexes of mammalian heme oxygenase, together with…”
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Implication for Using Heme Methyl Hyperfine Shifts as Indicators of Heme Seating as Related to Stereoselectivity in the Catabolism of Heme by Heme Oxygenase:  In-Plane Heme versus Axial His Rotation by Ogura, Hiroshi, Evans, John P, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…The triple mutant of the solubilized, 265-residue construct of human heme oxygenase, K18E/E29K/R183E-hHO, has been shown to redirect the exclusive…”
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H NMR Detection of Immobilized Water Molecules within a Strong Distal Hydrogen-Bonding Network of Substrate-Bound Human Heme Oxygenase-1 by Syvitski, Ray T, Li, Yiming, Auclair, Karine, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…Solution 1H NMR is used to probe the environments of the donor protons of eight strong hydrogen bonds on the distal side of the heme substrate in the…”
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A Double Mutant of Sperm Whale Myoglobin Mimics the Structure and Function of Elephant Myoglobin (∗) by Zhao, Xuefeng, Vyas, K., Nguyen, Bao D., Rajarathnam, Krishnakumar, Mar, Gerd N. La, Li, Tiansheng, Phillips, George N., Eich, Raymund F., Olson, John S., Ling, Jinshu, Bocian, David F.

“…The functional, spectral, and structural properties of elephant myoglobin and the L29F/H64Q mutant of sperm whale myoglobin have been compared in detail by…”
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Role of Propionates in Substrate Binding to Heme Oxygenase from Neisseria meningitidis: A Nuclear Magnetic Resonance Study by Peng, Dungeng, Ma, Li-Hua, Smith, Kevin M, Zhang, Xuhong, Sato, Michihiko, La Mar, Gerd N

“…Heme oxygenase (HO) cleaves hemin into biliverdin, iron, and CO. For mammalian HOs, both native hemin propionates are required for substrate binding and…”
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Solution NMR characterization of magnetic/electronic properties of azide and cyanide-inhibited substrate complexes of human heme oxygenase: Implications for steric ligand tilt by Peng, Dungeng, Ogura, Hiroshi, Ma, Li-Hua, Evans, John P., de Montellano, Paul R. Ortiz, La Mar, Gerd N.

“…Solution 2D 1H NMR was carried out on the azide-ligated substrate complex of human heme oxygenase, hHO, to provide information on the active site molecular…”
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Influence of Substrate Modification and C-Terminal Truncation on the Active Site Structure of Substrate-Bound Heme Oxygenase from Neisseriae meningitidis. A 1H NMR Study by Peng, Dungeng, Satterlee, James D, Ma, Li-Hua, Dallas, Jerry L, Smith, Kevin M, Zhang, Xuhong, Sato, Michihiko, La Mar, Gerd N

“…Heme oxygenase (HO), from the pathogenic bacterium N. meningitidis (NmHO), which secures host iron, shares many properties with mammalian HOs but also exhibits…”
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Application of the paramagnetic dipole field for solution NMR active site structure determination in low‐spin, cyanide‐inhibited ferric hemoproteins by La Mar, Gerd N.

“…The principles for the application of the paramagnetic dipolar field of low‐spin, cyanide‐inhibited ferrihemoproteins for determining active site structure are…”
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Solution NMR Characterization of the Electronic Structure and Magnetic Properties of High-Spin Ferrous Heme in Deoxy Myoglobin from Aplysia limacina by Ma, Dejian, Musto, Raffaella, Smith, Kevin M, La Mar, Gerd N

“…Solution 1H NMR has been used to elucidate the magnetic properties and electronic structure of the prosthetic group in high-spin, ferrous deoxy myoglobin from…”
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Coupling of the Distal Hydrogen Bond Network to the Exogenous Ligand in Substrate-Bound, Resting State Human Heme Oxygenase by Peng, Dungeng, Ogura, Hiroshi, Zhu, Wenfeng, Ma, Li-Hua, Evans, John P, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…Mammalian heme oxygenase (HO) possesses catalytically implicated distal ordered water molecules within an extended H-bond network, with one of the ordered…”
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Solution NMR Study of the Electronic and Molecular Structure of the Heme Cavity in High-Spin, Resting State Horseradish Peroxidase by de Ropp, Jeffrey S, Mandal, Pravat, Brauer, Samuel L, La Mar, Gerd N

“…Three resting state horseradish peroxidase isozymes (HRPC, HRPA1, and HRPA2) have been investigated by solution 1D and 2D NMR to determine the scope and…”
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NMR determination of the orientation of the magnetic susceptibility tensor in cyanometmyoglobin: a new probe of steric tilt of bound ligand by Emerson, S. Donald, La Mar, Gerd N

“…The experimentally determined paramagnetic dipolar shifts for noncoordinated amino acid side-chain protons in the heme pocket of sperm whale cyanometmyoglobin…”
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Solution 1H, 15N NMR Spectroscopic Characterization of Substrate-Bound, Cyanide-Inhibited Human Heme Oxygenase:  Water Occupation of the Distal Cavity by Li, Yiming, Syvitski, Ray T, Auclair, Karine, Ortiz de Montellano, Paul, La Mar, Gerd N

“…A solution NMR spectroscopic study of the cyanide-inhibited, substrate-bound complex of uniformly 15N-labeled human heme oxygenase, hHO, has led to…”
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1H NMR study of the effect of variable ligand on heme oxygenase electronic and molecular structure by Ma, Li-Hua, Liu, Yangzhong, Zhang, Xuhong, Yoshida, Tadashi, La Mar, Gerd N.

“…Heme oxygenase carries out stereospecific catabolism of protohemin to yield iron, CO and biliverdin. Instability of the physiological oxy complex has…”
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Solution structural characterization of cyanometmyoglobin: resonance assignment of heme cavity residues by two-dimensional NMR by Emerson, S. Donald, La Mar, Gerd N

“…Steady-state nuclear Overhauser effects (NOE), two-dimensional (2D) nuclear Overhauser effect spectroscopy (NOESY), and 2D spin correlation spectroscopy (COSY)…”
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Influence of the Distal His in Imparting Imidazolate Character to the Proximal His in Heme Peroxidase:  1H NMR Spectroscopic Study of Cyanide-Inhibited His42→Ala Horseradish Peroxidase by de Ropp, Jeffrey S, Sham, Simon, Asokan, Anbanandan, Newmyer, Sherri, Ortiz de Montellano, Paul R, La Mar, Gerd N

“…The functional higher oxidation states of heme peroxidases have been proposed to be stabilized by the significant imidazolate character of the proximal His…”
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Identification of localized redox states in plant-type two-iron ferredoxins using the nuclear Overhauser effect by Dugad, L. B, La Mar, Gerd N, Banci, Lucia, Bertini, Ivano

“…The homonuclear Overhauser effect (NOE), in conjunction with nonselective spin-lattice relaxation measurements, has been employed to assign the contact-shifted…”
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Proton NMR investigation of the oxidized three-iron clusters in the ferredoxins from the hyperthermophilic archae Pyrococcus furiosus and Thermococcus litoralis by Busse, Scott C, La Mar, Gerd N, Yu, Liping P, Howard, James B, Smith, Eugene T, Zhou, Zhi Hai, Adams, Michael W. W

“…The 3Fe forms of ferredoxins (Fd) from the hyperthermophilic archaebacteria Pyrococcus furiosus (Pf) and Thermococcus litoralis (Tl) have been investigated by…”
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