Search Results - "LYZNIK, A"

Site-specific recombination for genetic engineering in plants by LYZNIK, L. A, GORDON-KAMM, W. J, TAO, Y

“…Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible…”
Get full text

A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley ( Hordeum vulgare L.) by Gasparis, Sebastian, Kała, Maciej, Przyborowski, Mateusz, Łyżnik, Leszek A, Orczyk, Wacław, Nadolska-Orczyk, Anna

“…Genome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic…”
Get full text

Microinjection of the CRISPR/Cas9 editing system through the germ pore of a wheat microspore induces mutations in the target Ms2 gene by Szabała, Bartosz M., Święcicka, Magdalena, Łyżnik, Leszek A.

“…Background Microinjection is a direct procedure for delivering various compounds via micropipette into individual cells. Combined with the CRISPR/Cas9 editing…”
Get full text

FLP‐mediated recombination for use in hybrid plant production by Luo, Hong, Lyznik, L. Alexander, Gidoni, David, Hodges, Thomas K.

“…Summary We have studied the feasibility in Arabidopsis of using a site‐specific recombination system FLP/FRT, from the 2 μm plasmid of yeast, for making plant…”
Get full text

Pollen fertility restoration by nuclear gene Fr in CMS bean: nuclear-directed alteration of a mitochondrial population by He, S. (Purdue University, West Lafayette, IN.), Lyznik, A, Mackenzie, S

“…Two nuclear genes, Fr and Fr2, have been identified that restore pollen fertility to cytoplasmic male sterile (CMS) common bean (Phaseolus vulgaris L.) by…”
Get full text

Activity of yeast FLP recombinase in maize and rice protoplasts by Lyznik, Leszek A., Mitchell, Jon C., Hirayama, Lynne, Hodges, Thomas K.

“…We have demonstrated that a yeast FLP/FRT sitespecific recombination system functions In maize and rice protopiasts. FLP recombinase activity was monitored by…”
Get full text

The Elusive Plant Mitochondrion as a Genetic System by Mackenzie, Sally, He, Shichuan, Lyznik, Anna

Get full text

Evidence for a prokaryotic insertion-sequence contamination in eukaryotic séquences registered in différent databases by ASTUA-MONGE, G, LYZNIK, A, JONES, V, MACKENZIE, S. A, VALLEJOS, C. E

“…An insertion-sequence of prokaryotic origin was detected in a genomic clone obtained from a Phaseolus vulgaris bacterial artificial chromosome (BAC) library…”
Get full text

Heat‐inducible expression of FLP gene in maize cells by Lyznik, Leszek A., Hirayama, Lynne, Rao, K.V., Abad, Andre, Hodges, Thomas K.

“…The soybean heat‐shock gene promoter (Gmhsp 17.5‐E) has been used to direct expression of gusA and FLP genes in maize cells. At inducible temperatures, in…”
Get full text

Two nuclear-directed means of suppressing a dominant mitochondrial mutation in common bean by Sarria, R, Janska, H, Arrieta-Montiel, M, Lyznik, A, Mackenzie, S.A

“…The mitochondrial genome of the common bean is examined, and recent work demonstrating two means by which the pvsorf239 mitochondrial mutation in CMS common…”
Get full text

Dual-Domain, Dual-Targeting Organellar Protein Presequences in Arabidopsis Can Use Non-AUG Start Codons by Christensen, Alan C, Lyznik, Anna, Mohammed, Saleem, Elowsky, Christian G, Elo, Annakaisa, Yule, Ryan, Mackenzie, Sally A

“…The processes accompanying endosymbiosis have led to a complex network of interorganellar protein traffic that originates from nuclear genes encoding…”
Get full text

Stable co-transformation of maize protoplasts with gusA and neo genes by Lyznik, L.A. (Purdue Univ., West Lafayette, IN (USA). Dept. of Botany and Plant Pathology), Ryan, R.D, Ritchie, S.W, Hodges, T.K

“…An efficient co-transformation protocol using polyethylene glycol was developed for Zea mays L. (cv. A188 x BMS) protoplasts isolated from suspension culture…”
Get full text

Co-transformation of indica rice protoplasts with gusA and neo genes by Peng, J, Lyznik, L.A, Lee, L, Hodges, T.K

“…Protoplasts of the indica rice (Oryza sativa L.) variety, IR54, were transiently transformed with the gusA gene and stably transformed with both the neo and…”
Get full text

Analysis of promoter activity from an alpha-zein gene 5' flanking sequence in transient expression assays by Thompson, G.A, Boston, R.S, Lyznik, L.A, Hodges, T.K, Larkins, B.A

“…Three DNA regions required for high levels of transcription were identified by transient gene expression analysis of the 5' flanking region of a 19 kDa…”
Get full text

Tracing evolutionary and developmental implications of mitochondrial stoichiometric shifting in the common bean by Arrieta-Montiel, M, Lyznik, A, Woloszynska, M, Janska, H, Tohme, J, Mackenzie, S

“…The recombination and copy number shifting activities of the plant mitochondrial genome are widely documented across plant genera, but these genome processes…”
Get full text

Simplified procedure for transient transformation of plant protoplasts using polyethylene glycol treatment by Lyznik, L A, Peng, J Y, Hodges, T K

“…A modification of the polyethylene glycol-mediated transformation procedure which eliminates the manual polyethylene glycol dilution step is presented. A…”
Get more information

Nuclear Genes That Encode Mitochondrial Proteins for DNA and RNA Metabolism Are Clustered in the Arabidopsis Genome by Elo, Annakaisa, Lyznik, Anna, Gonzalez, Delkin O., Kachman, Stephen D., MacKenzie, Sally A.

“…The plant mitochondrial genome is complex in structure, owing to a high degree of recombination activity that subdivides the genome and increases genetic…”
Get full text

Gene targeting for chromosome engineering applications in eukaryotic cells by Lyznik, Leszek A, Dress, Virginia

“…As biotechnology advances, there is an increasing need to develop new technologies that may assist in more precise genetic engineering manipulations. Whether a…”
Get more information

FLP-Mediated Recombination of FRT Sites in the Maize Genome by Lyznik, L. Alexander, Rao, K. V., Hodges, Thomas K.

“…Molecular evidence is provided for genomic recombinations in maize cells induced by the yeast FLP/FRT site-specific recombination system. The FLP protein…”
Get full text

Stable transformation of maize: the impact of feeder cells on protoplast growth and transformation efficiency by LYZNIK, L. A, KAMO, K. K, GRIMES, H. D, RYAN, R, KA-LAI CHANG, HODGES, T. K

“…The importance of cell culture conditions, including the use of feeder cells, on protoplast growth and transformation in maize (Zea mays L.) was investigated…”
Get full text