Search Results - "LYND, Lee R"
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Ethanol production by engineered thermophiles
Published in Current opinion in biotechnology (01-06-2015)“…Graphical abstract…”
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Transformation of Clostridium thermocellum by electroporation
Published in Methods in enzymology (2012)“…In this work, we provide detailed instructions for transformation of Clostridium thermocellum by electroporation. In addition, we describe two schemes for…”
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Recent progress in consolidated bioprocessing
Published in Current opinion in biotechnology (01-06-2012)“…Highlights ► Substantial progress has been made in the development of CBP. ► Production of ethanol at high yield and titer using ‘native strategy’. ►…”
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Development of a thermophilic coculture for corn fiber conversion to ethanol
Published in Nature communications (22-04-2020)“…The fiber in corn kernels, currently unutilized in the corn to ethanol process, represents an opportunity for introduction of cellulose conversion technology…”
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Growth-uncoupled propanediol production in a Thermoanaerobacterium thermosaccharolyticum strain engineered for high ethanol yield
Published in Scientific reports (10-02-2023)“…Cocultures of engineered thermophilic bacteria can ferment lignocellulose without costly pretreatment or added enzymes, an ability that can be exploited for…”
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Metaproteomics reveals enzymatic strategies deployed by anaerobic microbiomes to maintain lignocellulose deconstruction at high solids
Published in Nature communications (05-07-2022)“…Economically viable production of cellulosic biofuels requires operation at high solids loadings—on the order of 15 wt%. To this end we characterize Nature’s…”
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Ethanol production from paper sludge by simultaneous saccharification and co-fermentation using recombinant xylose-fermenting microorganisms
Published in Biotechnology and bioengineering (01-10-2010)“…Simultaneous saccharification and co-fermentation (SSCF) of waste paper sludge to ethanol was investigated using two recombinant xylose-fermenting microbes:…”
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High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
Published in Applied and Environmental Microbiology (01-12-2011)“…OA Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley…”
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Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of Clostridium thermocellum
Published in PloS one (07-02-2014)“…Large-scale production of lignocellulosic biofuel is a potential solution to sustainably meet global energy needs. One-step consolidated bioprocessing (CBP) is…”
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Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield
Published in Proceedings of the National Academy of Sciences - PNAS (16-09-2008)“…We report engineering Thermoanaerobacterium saccharolyticum, a thermophilic anaerobic bacterium that ferments xylan and biomass-derived sugars, to produce…”
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The grand challenge of cellulosic biofuels
Published in Nature biotechnology (01-10-2017)“…Why cellulosic biofuels have fallen short of expectations and what we can do about it…”
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Enzyme-Microbe Synergy During Cellulose Hydrolysis by Clostridium thermocellum
Published in Proceedings of the National Academy of Sciences - PNAS (31-10-2006)“…Specific cellulose hydrolysis rates (g of cellulose/g of cellulase per h) were shown to be substantially higher (2.7- to 4.7-fold) for growing cultures of…”
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Fractionating recalcitrant lignocellulose at modest reaction conditions
Published in Biotechnology and bioengineering (01-06-2007)“…Effectively releasing the locked polysaccharides from recalcitrant lignocellulose to fermentable sugars is among the greatest technical and economic barriers…”
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Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum
Published in Proceedings of the National Academy of Sciences - PNAS (16-08-2011)“…Clostridium thermocellum is a thermophilic, obligately anaerobic, Gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass…”
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Development of both type I–B and type II CRISPR/Cas genome editing systems in the cellulolytic bacterium Clostridium thermocellum
Published in Metabolic engineering communications (01-06-2020)“…The robust lignocellulose-solubilizing activity of C. thermocellum makes it a top candidate for consolidated bioprocessing for biofuel production. Genetic…”
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The redox-sensing protein Rex modulates ethanol production in Thermoanaerobacterium saccharolyticum
Published in PloS one (05-04-2018)“…Thermoanaerobacterium saccharolyticum is a thermophilic anaerobe that has been engineered to produce high amounts of ethanol, reaching ~90% theoretical yield…”
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defined growth medium with very low background carbon for culturing Clostridium thermocellum
Published in Journal of industrial microbiology & biotechnology (01-06-2012)“…A growth medium was developed for cultivation of Clostridium thermocellum ATCC 27405 in which “background” carbon present in buffers, reducing agents,…”
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Microbial cellulose utilization: fundamentals and biotechnology
Published in Microbiology and Molecular Biology Reviews (01-09-2002)“…Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of…”
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Comparative efficiency and driving range of light- and heavy-duty vehicles powered with biomass energy stored in liquid fuels or batteries
Published in Proceedings of the National Academy of Sciences - PNAS (04-03-2014)“…This study addresses the question, “When using cellulosic biomass for vehicular transportation, which field-to-wheels pathway is more efficient: that using…”
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