Search Results - "LUKAVSKY, P."

Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides by Lukavsky, Peter J, Puglisi, Joseph D

“…We present a fast and simple protocol for large-scale preparation and purification of RNA oligonucleotides. RNA oligonucleotides are prepared by in vitro…”
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Structures of two RNA domains essential for hepatitis C virus internal ribosome entry site function by Puglisi, Joseph D, Lukavsky, Peter J, Otto, Geoff A, Lancaster, Alissa M, Sarnow, Peter

“…Translation of the hepatitis C virus (HCV) polyprotein is initiated at an internal ribosome entry site (IRES) element in the 5′ untranslated region of HCV RNA…”
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NMR Study of 100 kDa HCV IRES RNA Using Segmental Isotope Labeling by Kim, Insil, Lukavsky, Peter J, Puglisi, Joseph D

“…RNA NMR is hindered by the large size of most biological RNAs. We present here a simple method for segmental isotopic labeling of an RNA fragment within the…”
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Ribosomal proteins mediate the hepatitis C virus IRES–HeLa 40S interaction by OTTO, GEOFF A., LUKAVSKY, PETER J., LANCASTER, ALISSA M., SARNOW, PETER, PUGLISI, JOSEPH D.

“…Translation of the hepatitis C virus genomic RNA is mediated by an internal ribosome entry site (IRES). The 330-nt IRES RNA forms a binary complex with the…”
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Structure and function of HCV IRES domains by Lukavsky, Peter J.

“…The HCV IRES is a highly structured RNA which mediates cap-independent translation initiation in higher eukaryotes. This function is encoded in conserved…”
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RNAPack: An Integrated NMR Approach to RNA Structure Determination by Lukavsky, Peter J., Puglisi, Joseph D.

“…Over the last decade, a vast number of useful nuclear magnetic resonance (NMR) experiments have been developed and successfully employed to determine the…”
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Bicaudal-D uses a parallel, homodimeric coiled coil with heterotypic registry to coordinate recruitment of cargos to dynein by Liu, Yang, Salter, Hannah K, Holding, Andrew N, Johnson, Christopher M, Stephens, Elaine, Lukavsky, Peter J, Walshaw, John, Bullock, Simon L

“…Cytoplasmic dynein is the major minus end-directed microtubule motor in eukaryotes. However, there is little structural insight into how different cargos are…”
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Thermodynamic Stability and Structural Features of the J4/5 Loop in a Pneumocystis carinii Group I Intron by Schroeder, Susan J, Fountain, Matthew A, Kennedy, Scott D, Lukavsky, Peter J, Puglisi, Joseph D, Krugh, Thomas R, Turner, Douglas H

“…The J4/5 loop of the group I intron in the mouse-derived fungal pathogen Pneumocystis carinii is the docking site for the first step of the RNA-catalyzed…”
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Structure of the most conserved internal loop in SRP RNA by Schmitz, Uli, James, Thomas L, Lukavsky, Peter, Walter, Peter

“…The signal recognition particle (SRP) directs translating ribosomes to the protein translocation apparatus of endoplasmic reticulum (ER) membrane or the…”
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Structure of HCV IRES domain II determined by NMR by Puglisi, Joseph D, Lukavsky, Peter J, Kim, Insil, Otto, Geoff A

“…Complex RNA structures regulate many biological processes, but are often too large for structure determination by NMR methods. The 5' untranslated region (5'…”
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Complementary Segmental Labeling of Large RNAs:  Economic Preparation and Simplified NMR Spectra for Measurement of More RDCs by Tzakos, Andreas G, Easton, Laura E, Lukavsky, Peter J

“…NMR structure determination of large RNAs is often restricted by limited RDC information caused by chemical shift degeneracy. We established a general, time-…”
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Structure of the phylogenetically most conserved domain of SRP RNA by SCHMITZ, ULI, BEHRENS, STEFAN, FREYMANN, DOUG M., KEENAN, ROBERT J., LUKAVSKY, PETER, WALTER, PETER, JAMES, THOMAS L.

“…The signal recognition particle (SRP) is a phylogenetically conserved ribonucleoprotein required for cotranslational targeting of proteins to the membrane of…”
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Structure determination of large biological RNAs by Lukavsky, Peter J, Puglisi, Joseph D

“…Complex RNA structures regulate many biological processes but are often too large for structure determination by nuclear magnetic resonance (NMR) methods. We…”
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Complete Assignment of the13C NMR Spectra of Aryl Diazinyl Ketones and Aryl Diazinyl Ketoxime Ethers by Heinisch, G., Langer, T., Lukavsky, P.

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On the Bioisosteric Potential of Diazines:  Diazine Analogues of the Combined Thromboxane A2 Receptor Antagonist and Synthetase Inhibitor Ridogrel by Heinisch, Gottfried, Holzer, Wolfgang, Kunz, Friedbert, Langer, Thierry, Lukavsky, Peter, Pechlaner, Christoph, Weissenberger, Hans

“…In this SAR study the bioisosteric potential of diazines in the field of combined antithrombotic thromboxane A2 synthetase inhibitors and receptor antagonists…”
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Complete Assignment of the 13C NMR Spectra of Diazinyl-Substituted Ureas and Thioureas by HEINISCH, G., LUKAVSKY, P., MATUSZCZAK, B., RAKOWITZ, D.

“…The total assignment of the 13C NMR spectra of novel diazine derived ureas and thioureas is reported. © 1997 by John Wiley & Sons, Ltd…”
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Complete Assignment of the13C NMR Spectra of Diazinyl-Substituted Ureas and Thioureas by HEINISCH, G., LUKAVSKY, P., MATUSZCZAK, B., RAKOWITZ, D.

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A series of diarylsubstituted oximes as potential substrate for new aldose reductase inhibitors by Rakowitz, Dietmar, Heinisch, Gottfried, Lukavsky, Peter, Kiendler, Sigrid, Trenkwalder, Cornelia, Barlocco, Daniela, Rastelli, Giulio, Costantino, Luca

“…In the course of our research aimed at the discovery of new compounds acting as aldose reductase inhibitors, we tested a series of some (E)‐ and…”
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Determinants of Hepatitis C Translational Initiation in Vitro, in Cultured Cells and Mice by McCaffrey, Anton P., Ohashi, Kazuo, Meuse, Leonard, Shen, Shiliang, Lancaster, Alissa M., Lukavsky, Peter J., Sarnow, Peter, Kay, Mark A.

“…Hepatitis C virus (HCV) is an RNA virus infecting 1 in every 40 people worldwide. Development of new therapeutics for treating HCV has been hampered by the…”
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Affinity purification of eukaryotic 48S initiation complexes by Locker, Nicolas, Easton, Laura E, Lukavsky, Peter J

“…In vitro assembly of translation initiation complexes from higher eukaryotes requires purification of ribosomal subunits, eukaryotic initiation factors, and…”
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