The histological validation of post mortem magnetic resonance imaging-determined hippocampal volume in Alzheimer's disease

The histological validation of post mortem magnetic resonance imaging-determined hippocampal volume in Alzheimer's disease

For 11 AD cases and four normal elderly controls, post mortem volumes of the hippocampal subdivisions were calculated by using magnetic resonance imaging and histological sections. After at least six weeks of fixation in formalin, brains were examined on a 1.5-T Philips Gyroscan imager producing T1-...

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Bibliographic Details
Published in:Neuroscience Vol. 95; no. 3; pp. 721 - 725
Main Authors: BOBINSKI, M, DE LEON, M. J, WEGIEL, J, DESANTI, S, CONVIT, A, LOUIS, L. A. S, RUSINEK, H, WISNIEWSKI, H. M
Format: Journal Article
Language:English
Published: Oxford Elsevier 2000
Subjects:
Aged
Alzheimer Disease - pathology
Biological and medical sciences
Cadaver
Cell Count
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Hippocampus - pathology
Humans
Magnetic Resonance Imaging
Medical sciences
Neurology
Neurons - pathology
Parahippocampal Gyrus - pathology
Reference Values
Human
Nervous system diseases
Alzheimer disease
Central nervous system
Postmortem
Histology
Nuclear magnetic resonance imaging
Cerebral disorder
Volume
Imaging
Central nervous system disease
Degenerative disease
Hippocampus
Brain (vertebrata)
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Summary:For 11 AD cases and four normal elderly controls, post mortem volumes of the hippocampal subdivisions were calculated by using magnetic resonance imaging and histological sections. After at least six weeks of fixation in formalin, brains were examined on a 1.5-T Philips Gyroscan imager producing T1-weighted coronal images with a 3-mm slice thickness. Brains were then processed and embedded in paraffin. Serial coronal sections, 3 mm apart and stained with Cresyl Violet, were used for the planimetry and unbiased estimation of the total numbers of neurons in the hippocampal subdivisions. For all 15 cases, magnetic resonance imaging- and histology-based measurements were performed along the whole rostrocaudal extent of the hippocampal formation and included three subvolumes: (i) the hippocampus (CA1-CA4 and the dentate gyrus); (ii) hippocampus/subiculum; and (iii) hippocampus/parahippocampal gyrus. After controlling for shrinkage, strong correlations were found between magnetic resonance imaging and histological measurements for the hippocampus (r = 0.97, P < 0.001), hippocampus/subiculum (r = 0.95, P < 0.001) and hippocampus/parahippocampal gyrus (r = 0.89, P < 0.001). We also calculated the total number of neurons in the hippocampus and hippocampus/subiculum subvolumes. Strong correlations between the magnetic resonance imaging subvolumes and neuronal counts were found for the hippocampus (r = 0.90, P < 0.001) and the hippocampus/subiculum subvolume (r = 0.84, P < 0.001). We conclude that very accurate volumetric measurements of the whole hippocampal formation can be obtained by using a magnetic resonance imaging protocol. Moreover, the strong correlations between magnetic resonance imaging-based hippocampal volumes and neuronal numbers suggest the anatomical validity of magnetic resonance imaging volume measurements.
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ISSN:0306-4522
1873-7544