Search Results - "LLOSA, Matxalen"

Recruitment of heterologous substrates by bacterial secretion systems for transkingdom translocation by Guzmán-Herrador, Dolores L, Fernández-Gómez, Andrea, Llosa, Matxalen

“…Bacterial secretion systems mediate the selective exchange of macromolecules between bacteria and their environment, playing a pivotal role in processes such…”
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Chloramphenicol Selection of IS10 Transposition in the cat Promoter Region of Widely Used Cloning Vectors by González-Prieto, Coral, Agúndez, Leticia, Llosa, Matxalen

“…The widely used pSU8 family of cloning vectors is based on a p15A replicon and a chloramphenicol acetyltransferase (cat) gene conferring chloramphenicol…”
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Conjugative DNA Transfer From E. coli to Transformation-Resistant Lactobacilli by Samperio, Sara, Guzmán-Herrador, Dolores L, May-Cuz, Rigoberto, Martín, Maria Cruz, Álvarez, Miguel A, Llosa, Matxalen

“…Lactic acid bacteria (LAB) belonging to the genus classically known as , recently split into 25 different genera, include many relevant species for the food…”
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Translocation Motif in Relaxase TrwC Specifically Affects Recruitment by Its Conjugative Type IV Secretion System by Alperi, Anabel, Larrea, Delfina, Fernández-González, Esther, Dehio, Christoph, Zechner, Ellen L, Llosa, Matxalen

“…Type IV secretion system (T4SS) substrates are recruited through a translocation signal that is poorly defined for conjugative relaxases. The relaxase TrwC of…”
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Exchange of functional domains between a bacterial conjugative relaxase and the integrase of the human adeno-associated virus by Agúndez, Leticia, Zárate-Pérez, Francisco, Meier, Anita F, Bardelli, Martino, Llosa, Matxalen, Escalante, Carlos R, Linden, R Michael, Henckaerts, Els

“…Endonucleases of the HUH family are specialized in processing single-stranded DNA in a variety of evolutionarily highly conserved biological processes related…”
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DNA Delivery and Genomic Integration into Mammalian Target Cells through Type IV A and B Secretion Systems of Human Pathogens by Guzmán-Herrador, Dolores L, Steiner, Samuel, Alperi, Anabel, González-Prieto, Coral, Roy, Craig R, Llosa, Matxalen

“…We explore the potential of bacterial secretion systems as tools for genomic modification of human cells. We previously showed that foreign DNA can be…”
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Bacterial conjugation: a two‐step mechanism for DNA transport by Llosa, Matxalen, Gomis‐Rüth, F. Xavier, Coll, Miquel, Cruz, Fernando de la

“…Summary Bacterial conjugation is a promiscuous DNA transport mechanism. Conjugative plasmids transfer themselves between most bacteria, thus being one of the…”
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Conjugative Coupling Proteins Interact with Cognate and Heterologous VirB10-like Proteins while Exhibiting Specificity for Cognate Relaxosomes by Llosa, Matxalen, Zunzunegui, Sandra, de la Cruz, Fernando

“…Conjugative coupling proteins (CPs) are proposed to play a role in connecting the relaxosome to a type IV secretion system (T4SS) during bacterial conjugation…”
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Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase by Agúndez, Leticia, González-Prieto, Coral, Machón, Cristina, Llosa, Matxalen

“…Bacterial conjugation is a mechanism for horizontal DNA transfer between bacteria which requires cell to cell contact, usually mediated by self-transmissible…”
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Transfer of R388 Derivatives by a Pathogenesis-Associated Type IV Secretion System into both Bacteria and Human Cells by Fernández-González, Esther, de Paz, Héctor D, Alperi, Anabel, Agúndez, Leticia, Faustmann, Marco, Sangari, Félix J, Dehio, Christoph, Llosa, Matxalen

“…Bacterial type IV secretion systems (T4SSs) are involved in processes such as bacterial conjugation and protein translocation to animal cells. In this work, we…”
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Functional Dissection of the Conjugative Coupling Protein TrwB by DE PAZ, Héctor D, LARREA, Delfina, ZUNZUNEGUI, Sandra, DEHIO, Christoph, DE LA CRUZ, Fernando, LLOSA, Matxalen

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Site-Specific Recombinase and Integrase Activities of a Conjugative Relaxase in Recipient Cells by Draper, Olga, Carolina Elvira César, Cristina Machón, de la Cruz, Fernando, Llosa, Matxalen

“…Conjugative relaxases are the proteins that initiate bacterial conjugation by a site-specific cleavage of the transferred DNA strand. In vitro, they show…”
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TrwC-Mediated Site-Specific Recombination Is Controlled by Host Factors Altering Local DNA Topology by CESAR, Carolina Elvira, LLOSA, Matxalen

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Nuclear Targeting of a Bacterial Integrase That Mediates Site-Specific Recombination between Bacterial and Human Target Sequences by Agúndez, Leticia, Machón, Cristina, César, Carolina Elvira, Rosa-Garrido, Manuel, Delgado, M. Dolores, Llosa, Matxalen

“…TrwC is a bacterial protein involved in conjugative transfer of plasmid R388. It is transferred together with the DNA strand into the recipient bacterial cell,…”
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A new domain of conjugative relaxase TrwC responsible for efficient oriT‐specific recombination on minimal target sequences by César, Carolina Elvira, Machón, Cristina, De La Cruz, Fernando, Llosa, Matxalen

“…Summary We show that relaxase TrwC promotes recombination between two directly repeated oriTs while related relaxases TraI of F and pKM101 do not. Efficient…”
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Coupling Proteins in Type IV Secretion by Llosa, Matxalen, Alkorta, Itziar

“…Type IV coupling proteins (T4CPs) are essential constituents of most type IV secretion systems (T4SSs), and probably the most intriguing component in terms of…”
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The secret life of conjugative relaxases by Guzmán-Herrador, Dolores Lucía, Llosa, Matxalen

“…Conjugative relaxases are well-characterized proteins responsible for the site- and strand-specific endonucleolytic cleavage and strand transfer reactions…”
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Delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria through a type IV secretion system by Guzmán-Herrador, Dolores L, Fernández-Gómez, Andrea, Depardieu, Florence, Bikard, David, Llosa, Matxalen

“…CRISPR-associated (Cas) endonucleases and their derivatives are widespread tools for the targeted genetic modification of both prokaryotic and eukaryotic…”
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Editorial by Brom, Susana, Grohman, Elisabeth, Llosa, Matxalen

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Bacterial type IV secretion systems in human disease by Llosa, Matxalen, Roy, Craig, Dehio, Christoph

“…Summary Type IV secretion (T4S) systems are versatile machines involved in many processes relevant to bacterial virulence, such as horizontal DNA transfer and…”
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