Search Results - "LEONHARDT, Heinrich"

guide to super-resolution fluorescence microscopy by Schermelleh, Lothar, Heintzmann, Rainer, Leonhardt, Heinrich

“…For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies…”
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Heterochromatin drives compartmentalization of inverted and conventional nuclei by Falk, Martin, Feodorova, Yana, Naumova, Natalia, Imakaev, Maxim, Lajoie, Bryan R., Leonhardt, Heinrich, Joffe, Boris, Dekker, Job, Fudenberg, Geoffrey, Solovei, Irina, Mirny, Leonid A.

“…The nucleus of mammalian cells displays a distinct spatial segregation of active euchromatic and inactive heterochromatic regions of the genome 1 , 2 . In…”
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Comparative Analysis of Single-Cell RNA Sequencing Methods by Ziegenhain, Christoph, Vieth, Beate, Parekh, Swati, Reinius, Björn, Guillaumet-Adkins, Amy, Smets, Martha, Leonhardt, Heinrich, Heyn, Holger, Hellmann, Ines, Enard, Wolfgang

“…Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and medical questions. However, systematic comparisons of the performance…”
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Nanobodies and recombinant binders in cell biology by Helma, Jonas, Cardoso, M Cristina, Muyldermans, Serge, Leonhardt, Heinrich

“…Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has…”
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BigStitcher: reconstructing high-resolution image datasets of cleared and expanded samples by Hörl, David, Rojas Rusak, Fabio, Preusser, Friedrich, Tillberg, Paul, Randel, Nadine, Chhetri, Raghav K., Cardona, Albert, Keller, Philipp J., Harz, Hartmann, Leonhardt, Heinrich, Treier, Mathias, Preibisch, Stephan

“…Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that consist of many unaligned three-dimensional image tiles, which must be…”
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Nanobodies: Chemical Functionalization Strategies and Intracellular Applications by Schumacher, Dominik, Helma, Jonas, Schneider, Anselm F. L., Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…Nanobodies can be seen as next‐generation tools for the recognition and modulation of antigens that are inaccessible to conventional antibodies. Due to their…”
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CDK9-dependent RNA polymerase II pausing controls transcription initiation by Gressel, Saskia, Schwalb, Björn, Decker, Tim Michael, Qin, Weihua, Leonhardt, Heinrich, Eick, Dirk, Cramer, Patrick

“…Gene transcription can be activated by decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is achieved remains…”
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Usp7 and Uhrf1 control ubiquitination and stability of the maintenance DNA methyltransferase Dnmt1 by Qin, Weihua, Leonhardt, Heinrich, Spada, Fabio

“…In mammals Dnmt1 is the DNA methyltransferase chiefly responsible for maintaining genomic methylation patterns through DNA replication cycles, but how its…”
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A protein assembly mediates Xist localization and gene silencing by Pandya-Jones, Amy, Markaki, Yolanda, Serizay, Jacques, Chitiashvili, Tsotne, Mancia Leon, Walter R., Damianov, Andrey, Chronis, Constantinos, Papp, Bernadett, Chen, Chun-Kan, McKee, Robin, Wang, Xiao-Jun, Chau, Anthony, Sabri, Shan, Leonhardt, Heinrich, Zheng, Sika, Guttman, Mitchell, Black, Douglas. L., Plath, Kathrin

“…Nuclear compartments have diverse roles in regulating gene expression, yet the molecular forces and components that drive compartment formation remain largely…”
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Liquid-crystalline phase transitions in lipid droplets are related to cellular states and specific organelle association by Mahamid, Julia, Tegunov, Dimitry, Maiser, Andreas, Arnold, Jan, Leonhardt, Heinrich, Plitzko, Jürgen M., Baumeister, Wolfgang

“…Lipid droplets (LDs) are ubiquitous organelles comprising a central hub for cellular lipid metabolism and trafficking. This role is tightly associated with…”
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Identification of the elementary structural units of the DNA damage response by Natale, Francesco, Rapp, Alexander, Yu, Wei, Maiser, Andreas, Harz, Hartmann, Scholl, Annina, Grulich, Stephan, Anton, Tobias, Hörl, David, Chen, Wei, Durante, Marco, Taucher-Scholz, Gisela, Leonhardt, Heinrich, Cardoso, M. Cristina

“…Histone H2AX phosphorylation is an early signalling event triggered by DNA double-strand breaks (DSBs). To elucidate the elementary units of…”
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FUS-dependent liquid-liquid phase separation is important for DNA repair initiation by Levone, Brunno R, Lenzken, Silvia C, Antonaci, Marco, Maiser, Andreas, Rapp, Alexander, Conte, Francesca, Reber, Stefan, Mechtersheimer, Jonas, Ronchi, Antonella E, Mühlemann, Oliver, Leonhardt, Heinrich, Cardoso, M Cristina, Ruepp, Marc-David, Barabino, Silvia M L

“…RNA-binding proteins (RBPs) are emerging as important effectors of the cellular DNA damage response (DDR). The RBP FUS is implicated in RNA metabolism and DNA…”
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LBR and Lamin A/C Sequentially Tether Peripheral Heterochromatin and Inversely Regulate Differentiation by Solovei, Irina, Wang, Audrey S., Thanisch, Katharina, Schmidt, Christine S., Krebs, Stefan, Zwerger, Monika, Cohen, Tatiana V., Devys, Didier, Foisner, Roland, Peichl, Leo, Herrmann, Harald, Blum, Helmut, Engelkamp, Dieter, Stewart, Colin L., Leonhardt, Heinrich, Joffe, Boris

“…Eukaryotic cells have a layer of heterochromatin at the nuclear periphery. To investigate mechanisms regulating chromatin distribution, we analyzed…”
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Super-resolution in situ analysis of active ribosomal DNA chromatin organization in the nucleolus by Maiser, Andreas, Dillinger, Stefan, Längst, Gernot, Schermelleh, Lothar, Leonhardt, Heinrich, Németh, Attila

“…Ribosomal RNA (rRNA) transcription by RNA polymerase I (Pol I) is the first key step of ribosome biogenesis. While the molecular mechanisms of rRNA…”
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Role of Cdc48/p97 as a SUMO-targeted segregase curbing Rad51–Rad52 interaction by Bergink, Steven, Ammon, Tim, Kern, Maximilian, Schermelleh, Lothar, Leonhardt, Heinrich, Jentsch, Stefan

“…The segregase Cdc48 (also called p97 or VCP) extracts ubiquitylated proteins from their environment. Jentsch and colleagues demonstrate that Cdc48 binds…”
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In vivo control of CpG and non-CpG DNA methylation by DNA methyltransferases by Arand, Julia, Spieler, David, Karius, Tommy, Branco, Miguel R, Meilinger, Daniela, Meissner, Alexander, Jenuwein, Thomas, Xu, Guoliang, Leonhardt, Heinrich, Wolf, Verena, Walter, Jörn

“…The enzymatic control of the setting and maintenance of symmetric and non-symmetric DNA methylation patterns in a particular genome context is not well…”
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Cysteine‐Selective Phosphonamidate Electrophiles for Modular Protein Bioconjugations by Kasper, Marc‐André, Glanz, Maria, Stengl, Andreas, Penkert, Martin, Klenk, Simon, Sauer, Tom, Schumacher, Dominik, Helma, Jonas, Krause, Eberhard, Cardoso, M. Cristina, Leonhardt, Heinrich, Hackenberger, Christian P. R.

“…We describe a new technique in protein synthesis that extends the existing repertoire of methods for protein modification: A chemoselective reaction that…”
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Methylation of DNA Ligase 1 by G9a/GLP Recruits UHRF1 to Replicating DNA and Regulates DNA Methylation by Ferry, Laure, Fournier, Alexandra, Tsusaka, Takeshi, Adelmant, Guillaume, Shimazu, Tadahiro, Matano, Shohei, Kirsh, Olivier, Amouroux, Rachel, Dohmae, Naoshi, Suzuki, Takehiro, Filion, Guillaume J., Deng, Wen, de Dieuleveult, Maud, Fritsch, Lauriane, Kudithipudi, Srikanth, Jeltsch, Albert, Leonhardt, Heinrich, Hajkova, Petra, Marto, Jarrod A., Arita, Kyohei, Shinkai, Yoichi, Defossez, Pierre-Antoine

“…DNA methylation is an essential epigenetic mark in mammals that has to be re-established after each round of DNA replication. The protein UHRF1 is essential…”
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Cortical Constriction During Abscission Involves Helices of ESCRT-III—Dependent Filaments by Guizetti, Julien, Schermelleh, Lothar, Mäntler, Jana, Maar, Sandra, Poser, Ina, Leonhardt, Heinrich, Müller-Reichert, Thomas, Gerlich, Daniel W.

“…After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits…”
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Phosphorylation of TET Proteins Is Regulated via O-GlcNAcylation by the O-Linked N-Acetylglucosamine Transferase (OGT) by Bauer, Christina, Göbel, Klaus, Nagaraj, Nagarjuna, Colantuoni, Christian, Wang, Mengxi, Müller, Udo, Kremmer, Elisabeth, Rottach, Andrea, Leonhardt, Heinrich

“…TET proteins oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine and thus provide a possible means for active DNA…”
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