Abdominal Aortic Aneurysm Is a Specific Antigen-Driven T Cell Disease

: To determine whether monoclonal/oligoclonal T cells are present in abdominal aortic aneurysm (AAA) lesions, we amplified β‐chain T cell receptor (TCR) transcripts from these lesions by the nonpalindromic adaptor (NPA)‐polymerase chain reaction (PCR)/V‐β‐specific PCR followed by cloning and sequen...

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Published in:	Annals of the New York Academy of Sciences Vol. 1085; no. 1; pp. 224 - 235
Main Authors:	PLATSOUCAS, CHRIS D., LU, SONG, NWANESHIUDU, IFEYINWA, SOLOMIDES, CHARALAMBOS, AGELAN, ALEXIS, NTAOULA, NEKTARIA, PUREV, ENKHTUYA, LI, LI PING, KRATSIOS, PASCHALIS, MYLONAS, EFSTRATIOS, JUNG, WEON-JU, EVANS, KYLE, ROBERTS, SEAN, LU, YANDI, LAYVI, RICARDO, LIN, WAN LU, ZHANG, XIAOYING, GAUGHAN, JOHN, MONOS, DIMITRIOS S., OLESZAK, EMILIA L., WHITE, JOHN V.
Format:	Journal Article
Language:	English
Published:	Malden, USA Blackwell Publishing Inc 01-11-2006
Subjects:	abdominal aortic aneurysm alpha/beta T cell receptor and late-activation antigens Antigens - immunology Aortic Aneurysm, Abdominal - complications Aortic Aneurysm, Abdominal - genetics Aortic Aneurysm, Abdominal - immunology clonal expansions of T cells expression of early expression of early‐, intermediate‐, and late‐activation antigens gamma/delta T cell receptor Humans intermediate Lymphatic Diseases - complications Lymphatic Diseases - genetics Lymphatic Diseases - immunology oligoclonal T cells Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - immunology T cell mononuclear infiltrates Transcription, Genetic - genetics
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Summary:	: To determine whether monoclonal/oligoclonal T cells are present in abdominal aortic aneurysm (AAA) lesions, we amplified β‐chain T cell receptor (TCR) transcripts from these lesions by the nonpalindromic adaptor (NPA)‐polymerase chain reaction (PCR)/V‐β‐specific PCR followed by cloning and sequencing. Sequence analysis revealed the presence of substantial proportions of identical β‐chain TCR transcripts in AAA lesions in 9 of 10 patients examined, strongly suggesting the presence of oligoclonal populations of αβ TCR+ T cells. We have also shown the presence of oligoclonal populations of γδ TCR+ T cells in AAA lesions. Sequence analysis after appropriate PCR amplification and cloning revealed the presence of substantial proportions of identical VγI and VγII TCR transcripts in 15 of 15 patients examined, and of Vδ1 and Vδ2 TCR transcripts in 12 of 12 patients. These clonal expansions were very strong. All these clonal expansions were statistically significant by the binomial distribution. In other studies, we determined that mononuclear cells infiltrating AAA lesions express early‐ (CD69), intermediate‐ (CD25, CD38), and late‐ (CD45RO, HLA class II) activation antigens. These findings suggest that active ongoing inflammation is present in the aortic wall of patients with AAA. These results demonstrate that oligoclonal αβ TCR+ and γδ TCR+T cells are present in AAA lesions. These oligoclonal T cells have been clonally expanded in vivo in response to yet unidentified antigens. Although the antigenic specificity of these T cells remains to be determined, these T cells may play a significant role in the initiation and/or the propagation of the AAA. It appears that AAA is a specific antigen‐driven T cell disease.
Bibliography:	ArticleID:NYAS19 istex:EA49E248DF01657AC1620AB2FD3F872D708B11A3 ark:/67375/WNG-H9X2JNK0-V ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0077-8923 1749-6632 1930-6547
DOI:	10.1196/annals.1383.019