FGFR1 Emerges as a Potential Therapeutic Target for Lobular Breast Carcinomas

Purpose: Classic lobular carcinomas (CLC) account for 10% to 15% of all breast cancers. At the genetic level, CLCs show recurrent physical loss of chromosome16q coupled with the lack of E-cadherin ( CDH1 gene) expression. However, little is known about the putative therapeutic targets for these tumo...

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Published in:	Clinical cancer research Vol. 12; no. 22; pp. 6652 - 6662
Main Authors:	REIS-FILHO, Jorge Sergio, SIMPSON, Pete T, IRAVANI, Marjan, FENWICK, Kerry, WEBER, Barbara, HARDISSON, David, SCHMITT, Fernando Carlos, PALACIOS, Jose, LAKHANI, Sunil R, ASHWORTH, Alan, TURNER, Nicholas C, LAMBROS, Maryou Ballo, JONES, Chris, MACKAY, Alan, GRIGORIADIS, Anita, SARRIO, David, SAVAGE, Kay, DEXTER, Tim
Format:	Journal Article
Language:	English
Published:	Philadelphia, PA American Association for Cancer Research 15-11-2006
Subjects:	Antineoplastic agents Biological and medical sciences Biomarkers, Tumor - analysis Breast Neoplasms - genetics Breast Neoplasms - metabolism Cadherins - metabolism Carcinoma, Lobular - genetics Carcinoma, Lobular - metabolism Cell Line, Tumor Cell Survival chromogenic Chromosome Aberrations Chromosomes, Human, Pair 11 Chromosomes, Human, Pair 8 comparative genomic hybridization Down-Regulation Epigenesis, Genetic fluorescent Gene Amplification Gene Expression Regulation, Neoplastic Genetic Heterogeneity Gynecology. Andrology. Obstetrics Humans hybridization immunohistochemistry Immunohistochemistry - methods in situ invasive lobular carcinomas Mammary gland diseases Medical sciences microarrays Nucleic Acid Hybridization - methods Oligonucleotide Array Sequence Analysis - methods Pharmacology. Drug treatments Receptor, Fibroblast Growth Factor, Type 1 - genetics Receptor, Fibroblast Growth Factor, Type 1 - physiology Tumor Cells, Cultured Tumors Breast lobular carcinoma Mammary gland diseases Malignant tumor Targeting Fibroblast growth factor receptor 1
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Summary:	Purpose: Classic lobular carcinomas (CLC) account for 10% to 15% of all breast cancers. At the genetic level, CLCs show recurrent physical loss of chromosome16q coupled with the lack of E-cadherin ( CDH1 gene) expression. However, little is known about the putative therapeutic targets for these tumors. The aim of this study was to characterize CLCs at the molecular genetic level and identify putative therapeutic targets. Experimental Design: We subjected 13 cases of CLC to a comprehensive molecular analysis including immunohistochemistry for E-cadherin, estrogen and progesterone receptors, HER2/neu and p53; high-resolution comparative genomic hybridization (HR-CGH); microarray-based CGH (aCGH); and fluorescent and chromogenic in situ hybridization for CCND1 and FGFR1 . Results: All cases lacked the expression of E-cadherin, p53, and HER2, and all but one case was positive for estrogen receptors. HR-CGH revealed recurrent gains on 1q and losses on 16q (both, 85%). aCGH showed a good agreement with but higher resolution and sensitivity than HR-CGH. Recurrent, high level gains at 11q13 ( CCND1 ) and 8p12-p11.2 were identified in seven and six cases, respectively, and were validated with in situ hybridization. Examination of aCGH and the gene expression profile data of the cell lines, MDA-MB-134 and ZR-75-1, which harbor distinct gains of 8p12-p11.2, identified FGFR1 as a putative amplicon driver of 8p12-p11.2 amplification in MDA-MB-134. Inhibition of FGFR1 expression using small interfering RNA or a small-molecule chemical inhibitor showed that FGFR1 signaling contributes to the survival of MDA-MB-134 cells. Conclusions: Our findings suggest that receptor FGFR1 inhibitors may be useful as therapeutics in a subset of CLCs.
ISSN:	1078-0432 1557-3265
DOI:	10.1158/1078-0432.CCR-06-1164