Stage-specific expression of Gadd45 induced by X-irradiation in rat spermatogenesis

Purpose : Gadd45 is involved in the response to DNA damage in somatic cells. The effect of X-irradiation and chemical treatments on expression of Gadd45 and two other 53-regulated genes, p21 and cyclin-G, was studied in rat testis. Materials and methods : The reverse-transcriptase polymerase chain r...

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Published in:	International journal of radiation biology Vol. 78; no. 1; pp. 29 - 39
Main Authors:	West, A., Priante, G., Lährdetie, J.
Format:	Journal Article
Language:	English
Published:	London Informa UK Ltd 2002 Taylor & Francis
Subjects:	Animals Apoptosis Biological and medical sciences Biological effects of radiation Cyclin G Cyclin G1 Cyclins - biosynthesis Doxorubicin - pharmacology Etoposide - pharmacology Fundamental and applied biological sciences. Psychology GADD45 Proteins In Situ Hybridization Intracellular Signaling Peptides and Proteins Ionizing radiations Male Polymerase Chain Reaction Protein Biosynthesis Proteins - metabolism Proto-Oncogene Proteins p21(ras) - biosynthesis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA - metabolism RNA, Messenger - metabolism Seminiferous Tubules - drug effects Seminiferous Tubules - radiation effects Spermatogenesis - radiation effects Testis - metabolism Time Factors Tissues, organs and organisms biophysics X-Rays Animal model Rat Spermatogenesis Rodentia Experimental study Gene expression X ray irradiation Vertebrata Ionizing irradiation Mammalia Radiobiology TP53 Gene Animal Seminiferous tubule Biological effect Postreplicative repair
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Summary:	Purpose : Gadd45 is involved in the response to DNA damage in somatic cells. The effect of X-irradiation and chemical treatments on expression of Gadd45 and two other 53-regulated genes, p21 and cyclin-G, was studied in rat testis. Materials and methods : The reverse-transcriptase polymerase chain reaction (RT-PCR) was performed on testis extracts of control, X-irradiated (6Gy), etoposide (10 mg kg -1) and adriamycin (5mg kg -1) -treated rats. For stage-specific analysis, seminiferous tubules were isolated and segments representing the 14 epithelial stages were obtained. Results : In whole testis extracts, increases in Gadd45, p21 and cyclin-G expression were detectable after irradiation, but not after etoposide or adriamycin treatments. Analysis of fractions consisting of defined epithelial stages showed a high expression of Gadd45 in stages VII-XII and of p21 in stages VII-VIII. Irradiation significantly increased the level of Gadd45 mRNA in stages VI-VIII and of p21 mRNA in stages VI-I. Although no overall increase could be observed in whole testis samples of the etoposide-treated rat, stage-specific analysis revealed an induction of p21 expression in stages XIII-I. Gadd45 and cyclin-G mRNA were localized to spermatocytes and round spermatids known to express p21. Conclusions : Although X-irradiation, etoposide and adriamycinare known spermatogenic mutagens and activators of apoptosis, only X-rays induce slightly Gadd45 expression in testis. This small induction was very stage specific.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0955-3002 1362-3095
DOI:	10.1080/09553000110089982