Search Results - "L'Abbé, Denis"

Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells by Stuible, Matthew, Gervais, Christian, Lord-Dufour, Simon, Perret, Sylvie, L’Abbé, Denis, Schrag, Joseph, St-Laurent, Gilles, Durocher, Yves

“…•CHO cells are more efficient than 293 cells for expressing trimeric SARS-CoV-2 spike protein.•A trimerization domain and elimination of the S1/S2 furin site…”
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Transient Gene Expression in Suspension HEK293-EBNA1 Cells by L'Abbé, Denis, Bisson, Louis, Gervais, Christian, Grazzini, Eric, Durocher, Yves

“…Transient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant…”
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Impact of the temperature on the interactions between common variants of the SARS-CoV-2 receptor binding domain and the human ACE2 by Forest-Nault, Catherine, Koyuturk, Izel, Gaudreault, Jimmy, Pelletier, Alex, L’Abbé, Denis, Cass, Brian, Bisson, Louis, Burlacu, Alina, Delafosse, Laurence, Stuible, Matthew, Henry, Olivier, De Crescenzo, Gregory, Durocher, Yves

“…Several key mutations in the Spike protein receptor binding domain (RBD) have been identified to influence its affinity for the human Angiotensin-Converting…”
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Characterization of biotinylated human ACE2 and SARS-CoV-2 Omicron BA.4/5 spike protein reference materials by Stocks, Bradley B., Thibeault, Marie-Pier, L’Abbé, Denis, Umer, Muhammad, Liu, Yali, Stuible, Matthew, Durocher, Yves, Melanson, Jeremy E.

“…Accurate diagnostic and serology assays are required for the continued management of the COVID-19 pandemic yet spike protein mutations and intellectual…”
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Influence of variant-specific mutations, temperature and pH on conformations of a large set of SARS-CoV-2 spike trimer vaccine antigen candidates by Stuible, Matthew, Schrag, Joseph D., Sheff, Joey, Zoubchenok, Daria, Lord-Dufour, Simon, Cass, Brian, L’Abbé, Denis, Pelletier, Alex, Rossotti, Martin A., Tanha, Jamshid, Gervais, Christian, Maurice, Roger, El Bakkouri, Majida, Acchione, Mauro, Durocher, Yves

“…SARS-CoV-2 subunit vaccines continue to be the focus of intense clinical development worldwide. Protein antigens in these vaccines most commonly consist of the…”
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Unique epitope–antibody interactions in the intrinsically disordered proteoglycan-like domain of human carbonic anhydrase IX defined by high-resolution NMR combined with yeast surface display by Ni, Feng, Wu, Cunle, Xu, Ping, Wang, Ping, Fortin, Yves, Arbour, Melanie, Masson, Luke, L’Abbé, Denis, Acel, Andrea, Gosselin, Mylene, Lenferink, Anne E.G.

“…Carbonic anhydrase (CA)-IX is an extracellular enzyme that is essential in the adaptation of tumor cells to their increasingly more hypoxic and acidic…”
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A simplified polyethylenimine-mediated transfection process for large-scale and high-throughput applications by Raymond, Céline, Tom, Roseanne, Perret, Sylvie, Moussouami, Pascal, L’Abbé, Denis, St-Laurent, Gilles, Durocher, Yves

“…Transient gene expression in mammalian cells is a valuable alternative to stable cell lines for the rapid production of large amounts of recombinant proteins…”
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A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain by Forest-Nault, Catherine, Koyuturk, Izel, Gaudreault, Jimmy, Pelletier, Alex, L'Abbé, Denis, Cass, Brian, Bisson, Louis, Burlacu, Alina, Delafosse, Laurence, Stuible, Matthew, Henry, Olivier, De Crescenzo, Gregory, Durocher, Yves

“…Surface plasmon resonance (SPR)-based biosensing enables the characterization of protein-protein interactions. Several SPR-based approaches have been designed…”
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Hydrogen-deuterium exchange mass spectrometry reveals three unique binding responses of mAbs directed to the catalytic domain of hCAIX by Sheff, Joey G, Kelly, John F, Robotham, Anna, Sulea, Traian, Malenfant, Félix, L'Abbé, Denis, Duchesne, Mélanie, Pelletier, Alex, Lefebvre, Jean, Acel, Andrea, Parat, Marie, Gosselin, Mylene, Wu, Cunle, Fortin, Yves, Baardsnes, Jason, Van Faassen, Henk, Awrey, Shannon, Chafe, Shawn C, McDonald, Paul C, Dedhar, Shoukat, Lenferink, Anne E G

“…Human carbonic anhydrase (hCAIX), an extracellular enzyme that catalyzes the reversible hydration of CO , is often overexpressed in solid tumors. This enzyme…”
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SERS-based assay for multiplexed detection of cross-reactivity and persistence of antibodies against the spike of the native, P.1 and B.1.617.2 SARS-CoV-2 in non-hospitalised adults by Chisanga, Malama, Stuible, Matthew, Gervais, Christian, L'Abbé, Denis, Cass, Brian, Bisson, Louis, Pelletier, Alex, Lord-Dufour, Simon, Durocher, Yves, Boudreau, Denis, Trottier, Sylvie, Pelletier, Joelle N., Masson, Jean-Francois

“…Monitoring antibody response to SARS-CoV-2 is critical for assessing the humoral response, especially important considering the emergence of multiple…”
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Foot-and-Mouth Disease: Optimization, Reproducibility, and Scalability of High-Yield Production of Virus-Like Particles for a Next-Generation Vaccine by Mignaqui, Ana Clara, Ferella, Alejandra, Cass, Brian, Mukankurayija, Larissa, L'Abbé, Denis, Bisson, Louis, Sánchez, Cintia, Scian, Romina, Cardillo, Sabrina Beatriz, Durocher, Yves, Wigdorovitz, Andrés

“…Inactivated Foot-and-Mouth Disease (FMD) vaccine has proven to be effective in the control of the disease. However, its production has some disadvantages,…”
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Isolation and characterization of monoclonal antibodies against human carbonic anhydrase-IX by Lenferink, Anne E G, McDonald, Paul C, Cantin, Christiane, Grothé, Suzanne, Gosselin, Mylene, Baardsnes, Jason, Banville, Myriam, Lachance, Paul, Robert, Alma, Cepero-Donates, Yuneivy, Radinovic, Stevo, Salois, Patrick, Parat, Marie, Oamari, Hafida, Dulude, Annie, Patel, Mehul, Lafrance, Martin, Acel, Andrea, Bousquet-Gagnon, Nathalie, L'Abbé, Denis, Pelletier, Alex, Malenfant, Félix, Jaramillo, Maria, O'Connor-Mccourt, Maureen, Wu, Cunle, Durocher, Yves, Duchesne, Mélanie, Gadoury, Christine, Marcil, Anne, Fortin, Yves, Paul-Roc, Beatrice, Acchione, Maurizio, Chafe, Shawn C, Nemirovsky, Oksana, Lau, Joseph, Bénard, Francois, Dedhar, Shoukat

“…The architectural complexity and heterogeneity of the tumor microenvironment (TME) remains a substantial obstacle in the successful treatment of cancer…”
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Engineering and therapeutic application of single-chain bivalent TGF-β family traps by Zwaagstra, John C, Sulea, Traian, Baardsnes, Jason, Lenferink, Anne E G, Collins, Cathy, Cantin, Christiane, Paul-Roc, Béatrice, Grothe, Suzanne, Hossain, Sazzad, Richer, Louis-Philippe, L'Abbé, Denis, Tom, Roseanne, Cass, Brian, Durocher, Yves, O'Connor-McCourt, Maureen D

“…Deregulation of TGF-β superfamily signaling is a causative factor in many diseases. Here we describe a protein engineering strategy for the generation of…”
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High-throughput screening of effective siRNAs from RNAi libraries delivered via bacterial invasion by Zhao, Hui-Fen, Shen, Shi-Hsiang, L'Abbé, Denis, Jolicoeur, Normand, Wu, Meiqun, Li, Zhen, Yu, Zhenbao

“…Use of RNA interference (RNAi) as a reverse genetics tool for silencing genes in mammalian cells is achieved by in vitro transfection of small interfering RNAs…”
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Production and Characterization of a SARS-CoV-2 Nucleocapsid Protein Reference Material by Stocks, Bradley B, Thibeault, Marie-Pier, L'Abbé, Denis, Stuible, Matthew, Durocher, Yves, Melanson, Jeremy E

“…Rapid antigen tests have become a widely used COVID-19 diagnostic tool with demand accelerating in response to the highly contagious SARS-CoV-2 Omicron…”
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IGFBP-4 Anti-Angiogenic and Anti-Tumorigenic Effects Are Associated with Anti-Cathepsin B Activity by Moreno, María J, Ball, Marguerite, Rukhlova, Marina, Slinn, Jacqueline, L'Abbe, Denis, Iqbal, Umar, Monette, Robert, Hagedorn, Martin, O'Connor-McCourt, Maureen D, Durocher, Yves, Stanimirovic, Danica B

“…Abstract Insulin-like growth factor-binding protein 4 (IGFBP-4/IBP-4) has potent IGF-independent anti-angiogenic and antitumorigenic effects. In this study, we…”
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PILRα, a Novel Immunoreceptor Tyrosine-based Inhibitory Motif-bearing Protein, Recruits SHP-1 upon Tyrosine Phosphorylation and Is Paired with the Truncated Counterpart PILRβ by Mousseau, Darrell D., Banville, Denis, L'Abbé, Denis, Bouchard, Patrice, Shen, Shi-Hsiang

“…SHP-1-mediated dephosphorylation of protein tyrosine residues is central to the regulation of several cell signaling pathways, the specificity of which is…”
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Genetic variations of microRNAs in human cancer and their effects on the expression of miRNAs by Wu, Meiqun, Jolicoeur, Normand, Li, Zhen, Zhang, Linhua, Fortin, Yves, L'Abbe, Denis, Yu, Zhenbao, Shen, Shi-Hsiang

“…MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the posttranscriptional level to lead to mRNA degradation or repressed protein…”
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Unique epitope-antibody interactions in the intrinsically disordered proteoglycan-like domain of human carbonic anhydrase IX defined by high-resolution NMR combined with yeast surface display by Ni, Feng, Wu, Cunle, Xu, Ping, Wang, Ping, Fortin, Yves, Arbour, Melanie, Masson, Luke, L'Abbé, Denis, Acel, Andrea, Gosselin, Mylene, Lenferink, Anne E.G.

“…Carbonic anhydrase (CA)-IX is an extracellular enzyme that is essential in the adaptation of tumor cells to their increasingly more hypoxic and acidic…”
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IGFBP-4 Anti-Angiogenic and Anti-Tumorigenic Effects Are Associated with Anti-Cathepsin B Activity1 by Moreno, María J, Ball, Marguerite, Rukhlova, Marina, Slinn, Jacqueline, L'Abbe, Denis, Iqbal, Umar, Monette, Robert, Hagedorn, Martin, O'Connor-McCourt, Maureen D, Durocher, Yves, Stanimirovic, Danica B

“…Insulin-like growth factor-binding protein 4 (IGFBP-4/IBP-4) has potent IGF-independent anti-angiogenic and antitumorigenic effects. In this study, we…”
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