Motion analysis of optically trapped particles and cells using 2D Fourier analysis

Motion analysis of optically trapped particles and cells using 2D Fourier analysis

Motion analysis of optically trapped objects is demonstrated using a simple 2D Fourier transform technique. The displacements of trapped objects are determined directly from the phase shift between the Fourier transform of subsequent images. Using end- and side-view imaging, the stiffness of the tra...

Full description

Saved in:
Bibliographic Details
Published in:Optics express Vol. 20; no. 3; pp. 1953 - 1962
Main Authors: Kristensen, Martin Verner, Ahrendt, Peter, Lindballe, Thue Bjerring, Nielsen, Otto Højager Attermann, Kylling, Anton P, Karstoft, Henrik, Imparato, Alberto, Hosta-Rigau, Leticia, Stadler, Brigitte, Stapelfeldt, Henrik, Keiding, Søren Rud
Format: Journal Article
Language:English
Published: United States 30-01-2012
Subjects:
Animals
Cell Movement - physiology
Cell Movement - radiation effects
Cells, Cultured
Computer Simulation
Fourier Analysis
Mice
Models, Biological
Models, Chemical
Myoblasts - physiology
Myoblasts - radiation effects
Nanoparticles - chemistry
Nanoparticles - radiation effects
Optical Tweezers
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Motion analysis of optically trapped objects is demonstrated using a simple 2D Fourier transform technique. The displacements of trapped objects are determined directly from the phase shift between the Fourier transform of subsequent images. Using end- and side-view imaging, the stiffness of the trap is determined in three dimensions. The Fourier transform method is simple to implement and applicable in cases where the trapped object changes shape or where the lighting conditions change. This is illustrated by tracking a fluorescent particle and a myoblast cell, with subsequent determination of diffusion coefficients and the trapping forces.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.20.001953