The difference between cellulolytic 'culturomes' and microbiomes inhabiting two contrasting soil types

High-throughput 16S rRNA sequencing was performed to compare the microbiomes inhabiting two contrasting soil types-sod-podzolic soil and chernozem-and the corresponding culturome communities of potentially cellulolytic bacteria cultured on standard Hutchinson media. For each soil type, soil-specific...

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Bibliographic Details
Published in:	PloS one Vol. 15; no. 11; p. e0242060
Main Authors:	Evdokimova, Elizaveta V, Gladkov, Grigory V, Kuzina, Natalya I, Ivanova, Ekaterina A, Kimeklis, Anastasiia K, Zverev, Aleksei O, Kichko, Arina A, Aksenova, Tatyana S, Pinaev, Alexander G, Andronov, Evgeny E
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 20-11-2020 Public Library of Science (PLoS)
Subjects:	Acidic soils Agriculture Bacteria Bacteria - classification Bacteria - genetics Bacteria - growth & development Biodiversity Biology and Life Sciences Cellulolytic bacteria Cellulose Comparative analysis Composition Computer and Information Sciences Decomposition Deoxyribonucleic acid DNA DNA, Bacterial - genetics DNA, Ribosomal - genetics Earth Sciences Ecology and Environmental Sciences Evolution, Molecular High-Throughput Nucleotide Sequencing Laboratories Microbiological research Microbiology Microbiomes Microbiota (Symbiotic organisms) Microorganisms Phylogeny Podzolic soils Research and analysis methods RNA, Ribosomal, 16S - genetics rRNA 16S Sequence Analysis, DNA - methods Sod Soil dynamics Soil Microbiology Soil microorganisms Soil types Soils State budgets Taxonomy Russia United States > US
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Summary:	High-throughput 16S rRNA sequencing was performed to compare the microbiomes inhabiting two contrasting soil types-sod-podzolic soil and chernozem-and the corresponding culturome communities of potentially cellulolytic bacteria cultured on standard Hutchinson media. For each soil type, soil-specific microorganisms have been identified: for sod-podzolic soil-Acidothermus, Devosia, Phenylobacterium and Tumebacillus, and for chernozem soil-Sphingomonas, Bacillus and Blastococcus. The dynamics of differences between soil types for bulk soil samples and culturomes varied depending on the taxonomic level of the corresponding phylotypes. At high taxonomic levels, the number of common taxa between soil types increased more slowly for bulk soil than for culturome. Differences between soil-specific phylotypes were detected in bulk soil at a low taxonomic level (genus, species). A total of 13 phylotypes were represented both in soil and in culturome. No relationship was shown between the abundance of these phylotypes in soil and culturome.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 Competing Interests: The authors have declared that no competing interests exist.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0242060