Improvement of the cellular quality of cryopreserved bovine blastocysts accompanied by enhancement of the ATP-binding cassette sub-family B member 1 expression

► ABCB1 is expressed in the bovine blastocysts. ► The expression of ABCB1 in bovine blastocysts is highly induced by forskolin and rifampicin. ► The blastocyst survivability is improved by enhancement of ABCB1 expression. ► The high viability is accompanied by an increase in cell proliferation. The...

Full description

Saved in:

Bibliographic Details
Published in:	Reproductive toxicology (Elmsford, N.Y.) Vol. 35; pp. 17 - 24
Main Authors:	Mori, Miyuki, Kasa, Shojiro, Isozaki, Yoshihiro, Kamori, Tsugumitsu, Yamaguchi, Shoichiro, Ueda, Shuji, Kuwano, Toshio, Eguchi, Minako, Isayama, Keishiro, Nishimura, Shotaro, Tabata, Shoji, Yamauchi, Nobuhiko, Hattori, Masa-aki
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-01-2013
Subjects:	ABCB1 Animals Anti-Bacterial Agents - pharmacology ATP-Binding Cassette, Sub-Family B, Member 1 - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Blastocyst - metabolism Bovine blastocyst Cattle Cell proliferation Colforsin - pharmacology Cryopreservation Early development In vitro fertilization Oocytes - metabolism Rifampin - pharmacology RNA, Messenger - metabolism In vitro fertilization Cell proliferation Bovine blastocyst ABCB1 Early development
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	► ABCB1 is expressed in the bovine blastocysts. ► The expression of ABCB1 in bovine blastocysts is highly induced by forskolin and rifampicin. ► The blastocyst survivability is improved by enhancement of ABCB1 expression. ► The high viability is accompanied by an increase in cell proliferation. The ATP-binding cassette sub-family B member 1 (ABCB1) plays a critical role in maintaining the metabolic capability of cells as an efflux transporter that pumps xenobiotics out of cells. We investigated the effects of highly expressed ABCB1 on the development and viability of cryopreserved bovine embryos. The ABCB1 level in cultured bovine embryos was decreased during development to blastocyst-stage compared to germinal vesicle- and second metaphase-stage oocytes. When bovine embryos were cultured with forskolin and/or rifampicin, the ABCB1 level was significantly increased in blastocysts but embryo development was not significantly improved. After embryo cryopreservation, highly ABCB1-expressed blastocysts exhibited significant increases in viability and hatching rates. The high viability of the cryopreserved blastocysts was accompanied by a significant increase in cell proliferation during culture for 48h. Thus, ABCB1 is expressed in bovine oocytes and embryos, and the cellular quality of bovine blastocysts is improved by the enhancement of ABCB1 expression.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0890-6238 1873-1708
DOI:	10.1016/j.reprotox.2012.11.002