Search Results - "Kuwana, Takashi"

Concentration and total number of circulating primordial germ cells in Green-legged Partridgelike chicken embryos by Szczerba, Agata, Kuwana, Takashi, Bednarczyk, Marek

“…The Green-legged Partridgelike fowl is an old Polish indigenous breed of chicken. Primordial germ cells (PGCs) are one of the best sources of precursor cells…”
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Long-term culture of chicken primordial germ cells isolated from embryonic blood and production of germline chimaeric chickens by Naito, Mitsuru, Harumi, Takashi, Kuwana, Takashi

“…•A novel long-term culture for chicken PGCs especially for female PGCs was developed.•Feeder cells for culturing PGCs were not derived from xeno-animal…”
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In Vitro Culture of Chicken Circulating and Gonadal Primordial Germ Cells on a Somatic Feeder Layer of Avian Origin by Szczerba, Agata, Kuwana, Takashi, Paradowska, Michelle, Bednarczyk, Marek

“…The present study had two aims: (1) To develop a culture system that imitates a normal physiological environment of primordial germ cells (PGCs). There are two…”
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Serotonin receptors are selectively expressed in the avian germ cells and early embryos by Stępińska, Urszula, Kuwana, Takashi, Olszańska, Bożenna

“…The expression of nine serotonin (5-HT) receptor transcripts was studied using reverse transcription polymerase chain reaction (RT-PCR) in germ cells, cleavage…”
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Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System by Mitsuru Naito, Takashi Harumi, Takashi Kuwana

“…In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs…”
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Testicular and ovarian gonocytes from 20-day incubated chicken embryos contribute to germline lineage after transfer into bloodstream of recipient embryos by Naito, Mitsuru, Minematsu, Takeo, Harumi, Takashi, Kuwana, Takashi

“…The present study was conducted to elucidate whether testicular and ovarian gonocytes obtained from 20-day incubated chicken embryos (stage 45) have the…”
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Expression of GFP Gene in Cultured PGCs Isolated from Embryonic Blood and Incorporation into Gonads of Recipient Embryos by Mitsuru Naito, Takashi Harumi, Takashi Kuwana

“…The present study was conducted to develop a technique for generating primordial germ cells expressing GFP gene and introducing them into gonads of recipient…”
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Methylmercury analyses in biological materials by heating vaporization atomic absorption spectrometry by Miyamoto, Ken-ichiro, Kuwana, Takashi, Ando, Tetsuo, Yamamoto, Megumi, Nakano, Atsuhiro

“…To develop an analytical method for methylmercury (MeHg) compounds in biological samples (hair, blood, fish) with reproducibly high recovery of MeHg using…”
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In vitro Culture of Testicular and Ovarian Gonocytes Obtained from 19-day Incubated Chicken Embryos and Subsequent Colonization into Gonads of Recipient Embryos by Mitsuru Naito, Takashi Harumi, Takashi Kuwana

“…Testicular gonocytes differentiate into spermatogonia and spermatogonial stem cells could be established by culturing spermatogonia in vitro. Spermatogonial…”
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Preliminary Research on Mycoplasma synoviae Vertical Transmission Rate into Primordial Germ Cells by Manabu Onuma, Takashi Kuwana

“…The vertical transmission rate of Mycoplasma synoviae into primordial germ cells (PGCs) was evaluated. PGCs were collected from eggs (n=54) of M. synoviae…”
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Preliminary studies on developing a nested PCR assay for molecular diagnosis and identification of nematode (Heterakis isolonche) and trematode (Glaphyrostomum sp.) in Okinawa rail (Gallirallus okinawae) by Zhao, Chen, Onuma, Manabu, Asakawa, Mitsuhiko, Nagamine, Takashi, Kuwana, Takashi

“…To investigate and detect internal parasites in Okinawa rail (Gallirallus okinawae), a novel nested polymerase chain reaction with high level of sensitivity…”
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T-Cell Lymphoma in a Wild Okinawa Rail (Gallirallus okinawae) by NEAGARI, Yasuko, NAGAMINE, Takashi, Nakaya, Yumiko, ONUMA, Manabu, MURATA, Koichi, KUWANA, Takashi

“…The Okinawa rail (Gallirallus okinawae) is an endangered species that inhabits the northern part of Okinawa Main Island in southern Japan. A wild Okinawa rail…”
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Long Term in vitro Culture of Chicken Primordial Germ Cells Isolated from Embryonic Blood and Incorporation into Germline of Recipient Embryo by Mitsuru Naito, Takashi Harumi, Takashi Kuwana

“…The present study was carried out to develop a long-term in vitro culture system for chicken primordial germ cells (PGCs). PGCs were obtained from the blood of…”
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Preferential Migration of Transferred Primordial Germ Cells to Left Germinal Ridge of Recipient Embryos in Chickens by Mitsuru Naito, Takeo Minematsu, Takashi Harumi, Takashi Kuwana

“…Chicken primordial germ cells (PGCs) circulate in the bloodstream and migrate to germinal ridges. In order to assess the migration frequencies of PGCs to left…”
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Morphological Characteristics of Symmetrically Developed Ovaries in a 17-day Incubated Chicken Embryo by Mitsuru Naito, Takashi Harumi, Takashi Kuwana

“…Morphological characteristics of symmetrically developed ovaries observed in a 17-day incubated chicken embryo were analysed. The size and shape of the left…”
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BMP signaling during bone pattern determination in the developing limb by Kawakami, Y, Ishikawa, T, Shimabara, M, Tanda, N, Enomoto-Iwamoto, M, Iwamoto, M, Kuwana, T, Ueki, A, Noji, S, Nohno, T

“…To examine the role of BMP signaling during limb pattern formation, we isolated chicken cDNAs encoding type I (BRK-1 and BRK-2) and type II (BRK-3) receptors…”
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Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo by Mitsuru Naito, Takeo Minematsu, Takashi Harumi, Takashi Kuwana

“…The present study was carried out to develop techniques for introducing exogenous DNA into primordial germ cells (PGCs) and expressing the introduced DNA…”
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Migration of Avian Primordial Germ Cells toward the Gonadal Anlage by Kuwana, Takashi

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Conservation of a Threatened Indigenous Fowl (Kureko Dori) Using the Germline Chimeras Transplanted from Primordial Germ Cells by Takashi Kuwana, Takaharu Kawashima, Mitsuru Naito, Hiroaki Yamashita, Masaharu Matsuzaki, Toshinori Takano

“…This is the first successful report on producing the endangered domestic fowl, Kureko Dori (KD), which is a Kumamoto prefectural natural treasure, using…”
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developmental origin of primordial germ cells and the transmission of the donor-derived gametes in mixed-sex germline chimeras to the offspring in the chicken by Kagami, H, Tagami, T, Matsubara, Y, Harumi, T, Hanada, H, Maruyama, K, Sakurai, M, Kuwana, T, Naito, M

“…A novel system has been developed to determine the origin and development of primordial germ cells (PGCs) in avian embryos directly. Approximately 700 cells…”
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