Association Between Antiarrhythmic, Electrophysiological, and Antioxidative Effects of Melatonin in Ischemia/Reperfusion

Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion u...

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Published in:	International journal of molecular sciences Vol. 20; no. 24; p. 6331
Main Authors:	Sedova, Ksenia A, Bernikova, Olesya G, Cuprova, Julia I, Ivanova, Alexandra D, Kutaeva, Galina A, Pliss, Michael G, Lopatina, Ekaterina V, Vaykshnorayte, Marina A, Diez, Emiliano R, Azarov, Jan E
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 15-12-2019 MDPI
Subjects:	Action potential Adenosine triphosphate Animals Anti-Arrhythmia Agents - pharmacology Antiarrhythmics Antioxidants - pharmacology Arrhythmias, Cardiac - drug therapy Cardiac arrhythmia Cardiac Electrophysiology - methods Electrocardiography - drug effects Electrophysiological Phenomena - drug effects Enzymes Epicardium Fibrillation Heart Ventricles - drug effects Ischemia Male Melatonin Melatonin - pharmacology Metabolism Myocardial Reperfusion Injury - drug therapy Occlusion Oxidative stress Placebos Potassium Rats Rats, Wistar Regression analysis Reperfusion Reperfusion Injury - drug therapy Superoxide dismutase Tachycardia Ventricle Ventricular fibrillation Ventricular Fibrillation - drug therapy arrhythmia depolarization repolarization melatonin reperfusion oxidative stress ischemia
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Summary:	Melatonin is assumed to confer cardioprotective action via antioxidative properties. We evaluated the association between ventricular tachycardia and/or ventricular fibrillation (VT/VF) incidence, oxidative stress, and myocardial electrophysiological parameters in experimental ischemia/reperfusion under melatonin treatment. Melatonin was given to 28 rats (10 mg/kg/day, orally, for 7 days) and 13 animals received placebo. In the anesthetized animals, coronary occlusion was induced for 5 min followed by reperfusion with recording of unipolar electrograms from ventricular epicardium with a 64-lead array. Effects of melatonin on transmembrane potentials were studied in ventricular preparations of 7 rats in normal and "ischemic" conditions. Melatonin treatment was associated with lower VT/VF incidence at reperfusion, shorter baseline activation times (ATs), and activation-repolarization intervals and more complete recovery of repolarization times (RTs) at reperfusion (less baseline-reperfusion difference, ΔRT) ( < 0.05). Superoxide dismutase (SOD) activity was higher in the treated animals and associated with ΔRT ( = 0.001), whereas VT/VF incidence was associated with baseline ATs ( = 0.020). In vitro, melatonin led to a more complete restoration of action potential durations and resting membrane potentials at reoxygenation ( < 0.05). Thus, the antioxidative properties of melatonin were associated with its influence on repolarization duration, whereas the melatonin-related antiarrhythmic effect was associated with its oxidative stress-independent action on ventricular activation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1422-0067 1661-6596 1422-0067
DOI:	10.3390/ijms20246331