Search Results - "Kushnir, Susanna"

Enzyme-Directed Mutasynthesis: A Combined Experimental and Theoretical Approach to Substrate Recognition of a Polyketide Synthase by Sundermann, Uschi, Bravo-Rodriguez, Kenny, Klopries, Stephan, Kushnir, Susanna, Gomez, Hansel, Sanchez-Garcia, Elsa, Schulz, Frank

“…Acyltransferase domains control the extender unit recognition in Polyketide Synthases (PKS) and thereby the side-chain diversity of the resulting natural…”
Get full text

Identification of crucial bottlenecks in engineered polyketide biosynthesis by Grote, Marius, Kushnir, Susanna, Pryk, Niclas, Möller, David, Erver, Julian, Ismail-Ali, Ahmed, Schulz, Frank

“…The concept of combinatorial biosynthesis promises access to compound libraries based on privileged natural scaffolds. Ever since the elucidation of the…”
Get more information

Structure of doubly prenylated Ypt1:GDI complex and the mechanism of GDI-mediated Rab recycling by Pylypenko, Olena, Rak, Alexey, Durek, Thomas, Kushnir, Susanna, Dursina, Beatrice E, Thomae, Nicolas H, Constantinescu, Alexandru T, Brunsveld, Luc, Watzke, Anja, Waldmann, Herbert, Goody, Roger S, Alexandrov, Kirill

“…In eukaryotic cells Rab/Ypt GTPases represent a family of key membrane traffic controllers that associate with their targeted membranes via C‐terminally…”
Get full text

Data in support of substrate flexibility of a mutated acyltransferase domain and implications for polyketide biosynthesis by Klopries, Stephan, Bravo-Rodriguez, Kenny, Koopmans, Kyra R.M., Sundermann, Uschi, Yahiaoui, Samir, Arens, Julia, Kushnir, Susanna, Sanchez-Garcia, Elsa, Schulz, Frank

“…Enzyme-directed mutasynthesis is an emerging strategy for the targeted derivatization of natural products. Here, data on the synthesis of malonic acid…”
Get full text

Polyether Cyclization Cascade Alterations in Response to Monensin Polyketide Synthase Mutations by Heinrich, Sascha, Grote, Marius, Sievers, Sonja, Kushnir, Susanna, Schulz, Frank

“…The targeted manipulation of polyketide synthases has in recent years led to numerous new‐to‐nature polyketides. For type I polyketide synthases the response…”
Get full text

Substrate Flexibility of a Mutated Acyltransferase Domain and Implications for Polyketide Biosynthesis by Bravo-Rodriguez, Kenny, Klopries, Stephan, Koopmans, Kyra R.M., Sundermann, Uschi, Yahiaoui, Samir, Arens, Julia, Kushnir, Susanna, Schulz, Frank, Sanchez-Garcia, Elsa

“…Polyketides are natural products frequently used for the treatment of various diseases, but their structural complexity hinders efficient derivatization. In…”
Get full text

Minimally Invasive Mutagenesis Gives Rise to a Biosynthetic Polyketide Library by Kushnir, Susanna, Sundermann, Uschi, Yahiaoui, Samir, Brockmeyer, Andreas, Janning, Petra, Schulz, Frank

“…Not in the public domain: Site‐directed mutagenesis of megasynthases was the key to the generation of a library of polyketides in bacteria. Redox…”
Get full text

Predicted Incorporation of Non-native Substrates by a Polyketide Synthase Yields Bioactive Natural Product Derivatives by Bravo-Rodriguez, Kenny, Ismail-Ali, Ahmed F., Klopries, Stephan, Kushnir, Susanna, Ismail, Shehab, Fansa, Eyad K., Wittinghofer, Alfred, Schulz, Frank, Sanchez-Garcia, Elsa

“…The polyether ionophore monensin is biosynthesized by a polyketide synthase that delivers a mixture of monensins A and B by the incorporation of ethyl‐ or…”
Get full text

Development of DNA Sequencing: From the Genome of a Bacteriophage to That of a Neanderthal by Sundermann, Uschi, Kushnir, Susanna, Schulz, Frank

“…Them bones gonna rise again: Only a few weeks ago the Neandertal genome, deciphered from small amounts of ancient DNA, was published. This mileston was…”
Get full text

Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae by Kushnir, Susanna, Cirstea, Ion Cristian, Basiliya, Lyudmyla, Lupilova, Nataliya, Breitling, Reinhard, Alexandrov, Kirill

“…[Display omitted] ▶ The protozoon Leishmania tarentolae was developed as a host for recombinant protein production. ▶ We present a new type of expression…”
Get full text

Structure of Rab GDP-Dissociation Inhibitor in Complex with Prenylated YPT1 GTPase by Rak, Alexey, Pylypenko, Olena, Durek, Thomas, Watzke, Anja, Kushnir, Susanna, Brunsveld, Lucas, Waldmann, Herbert, Goody, Roger S., Alexandrov, Kirill

“…Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key membrane traffic regulators in eukaryotic cells whose function is governed by the…”
Get full text

Development of an inducible protein expression system based on the protozoan host Leishmania tarentolae by Kushnir, Susanna, Gase, Klaus, Breitling, Reinhard, Alexandrov, Kirill

“…Production of functional eukaryotic proteins in recombinant form is a bottle-neck in various post-genomic applications and in life science in general. At least…”
Get full text

Construction and analysis of Leishmania tarentolae transgenic strains free of selection markers by Mureev, Sergei, Kushnir, Susanna, Kolesnikov, Alexander A., Breitling, Reinhard, Alexandrov, Kirill

“…The trypanosomatid protozoan Leishmania tarentolae has been extensively used as a model system for studying causative agents of several tropical diseases and…”
Get full text

Aufbau einer biosynthetischen Polyketid‐Bibliothek durch minimalinvasive Mutagenese by Kushnir, Susanna, Sundermann, Uschi, Yahiaoui, Samir, Brockmeyer, Andreas, Janning, Petra, Schulz, Frank

“…Auf den Punkt mutiert: Eine Strategie der ortsgerichteten Mutagenese von Megasynthasen wurde zum Aufbau einer Polyketid‐Substanzbibliothek genutzt. Dabei…”
Get full text

Translational initiation in Leishmania tarentolae and Phytomonas serpens (Kinetoplastida) is strongly influenced by pre-ATG triplet and its 5′ sequence context by Lukeš, Julius, Paris, Zdeněk, Regmi, Sandesh, Breitling, Reinhard, Mureev, Sergey, Kushnir, Susanna, Pyatkov, Konstantin, Jirků, Milan, Alexandrov, Kirill A.

“…To investigate the influence of sequence context of translation initiation codon on translation efficiency in Kinetoplastida, we constructed a library of…”
Get full text

Die Entwicklung der DNA-Sequenzierung - vom Genom eines Bakteriophagen zum Neandertaler by Sundermann, Uschi, Kushnir, Susanna, Schulz, Frank

“…Knochenarbeit: Seit wenigen Wochen ist das Genom des Neandertalers bekannt, es wurde aus sehr geringen Mengen Jahrtausende alter DNA entziffert. Dieses…”
Get full text

Rapid Production of Functionalized Recombinant Proteins:  Marrying Ligation Independent Cloning and in Vitro Protein Ligation by Kushnir, Susanna, Marsac, Yoann, Breitling, Reinhard, Granovsky, Igor, Brok-Volchanskaya, Vera, Goody, Roger S, Becker, Christian F. W, Alexandrov, Kirill

“…Functional genomics and proteomics have been very active fields since the sequencing of several genomes was completed. To assign a physiological role to the…”
Get full text

Aufbau einer biosynthetischen Polyketid-Bibliothek durch minimalinvasive Mutagenese by Kushnir, Susanna, Sundermann, Uschi, Yahiaoui, Samir, Brockmeyer, Andreas, Janning, Petra, Schulz, Frank

Get full text